The Esr1cre knockin allele has Cre recombinase expression directed to estrogen receptor 1 alpha (Esr1 or ERα)-expressing cells by the endogenous Esr1 promoter/enhancer elements; specifically in the ventrolateral subdivision of the ventromedial hypothalamus that, like wildtype mice, is greater in females than males. Expression is also observed in arcuate nucleus (ARH). These Esr1-Cre knockin mice may be useful for studying the function of this ligand-inducible transcription factor in social interactions, neuronal differentiation, sympathetic nervous system development, hypothalamus, female reproductive organs (endometrium and ovarian stroma cells), and male efferent duct epithelial cells. They may also be useful for studying ERα-positive breast cancer and steroid hormone-induced malignancies. Esr1-Cre knockin mice are available on a C57BL/6N background (Stock No. 017911) and a C57BL/6J background (Stock No. 017913).
David J Anderson, California Institute of Technology
Genetic Background | Generation |
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N3+pN2F8
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing, Inducible) | Esr1 | estrogen receptor 1 (alpha) |
Starting at:
$278.00 Domestic price for female |
356.51 Domestic price for breeder pair |
The Esr1cre (Esr1-Cre) knockin allele harbors an attP-flanked 2A oligopeptide and Cre recombinase gene in the 3' UTR of the estrogen receptor 1 alpha gene (Esr1 or ERα). Under direction of the endogenous Esr1 promoter/enhancer elements, Cre recombinase expression is observed in a pattern similar to that of Esr1. The donating investigator specifically reports Cre recombinase expression in the ventrolateral subdivision of the ventromedial hypothalamus (VMHv1) that, like wildtype mice, is greater in females than males. Expression is also observed in arcuate nucleus (ARH). The donating investigator reports that heterozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In 2014, it is the experience of The Jackson Laboratory Repository colony managers that C57BL/6NJ-congenic Esr1-Cre knockin homozygotes of both sexes do not breed well.
ERα, a nuclear hormone receptor (NHR), binds to steroid hormones in the cytoplasm, causing their translocation into the nucleus and leading to increased transcription of target genes. These Esr1-Cre knockin mice may be useful for studying the function of this ligand-inducible transcription factor in neuronal differentiation, sympathetic nervous system development, hypothalamus, female reproductive organs (endometrium and ovarian stroma cells), and male efferent duct epithelial cells. They may also be useful for studying ERα-positive breast cancer and steroid hormone-induced malignancies.
The Esr1cre (Esr1-Cre) knockin allele was designed by Dr. David J. Anderson (California Institute of Technology) to insert an attP site, a 2A oligopeptide (mediates ribosomal skipping), the Cre recombinase gene, a frt-flanked PGKneo-polyA cassette, and an attP site within the 3' UTR of the estrogen receptor 1 alpha gene (Esr1). The targeting construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with 129S6/SvEvTac females to establish the colony. Heterozygous mice were backcrossed to C57BL/6N for two generations. Next, a heterozygous male was bred to ACT-FLPe mice (backcrossed onto C57BL/6 background) to remove the frt-flanked PGKneo-polyA cassette. The resulting Esr1-Cre knockin mice were bred to C57BL/6N mice for one more generation (and the ACT-FLPe transgene removed) prior to sending black mutant mice to The Jackson Laboratory Repository in 2012. Upon arrival at The Jackson Laboratory Repository, mutant mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for several generations using a marker-assisted speed congenic approach. As of April 2014, the C57BL/6NJ-congenic Esr1cre knockin colony has all markers that determine C57BL/6 substrains confirmed to be C57BL/6N allele-type. Overall, at least 148/149 markers (99%) are C57BL/6 allele-type. The single segregating marker is 129 allele-type on chromosome 10 near the targeted locus (consistent with contributions from the ES cell used in creating the targeted mutation).
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase is expressed in the ventrolateral subdivision of the ventromedial hypothalamus, and also in the arcuate nucleus. |
Allele Name | targeted mutation 1.1, David J Anderson |
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Allele Type | Targeted (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Esr1cre; Esr1cre |
Gene Symbol and Name | Esr1, estrogen receptor 1 (alpha) |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase is expressed in the ventrolateral subdivision of the ventromedial hypothalamus, and also in the arcuate nucleus. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 10 |
Molecular Note | The Esr1-cre targeting vector was designed to insert an attP site, a 2A oligopeptide (mediates ribosomal skipping), the Cre recombinase gene, a frt-flanked PGKneo-polyA cassette, and an attP site within the 3' UTR of the estrogen receptor 1 alpha gene (Esr1). The targeting construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Chimeric males were bred with 129S6/SvEvTac females to establish the colony. Heterozygotes were bred to ACT-FLPe mice to remove the frt-flanked PGKneo-polyA cassette. Mice were bred to remove the ACT-FLPe transgene from the background. |
In 2014, it is the experience of The Jackson Laboratory Repository colony managers that C57BL/6NJ-congenic Esr1-Cre knockin homozygotes of both sexes do not breed well.
Therefore when maintaining our live colony, heterozygous mice may be bred with wildtype mice from the colony or with C57BL/6NJ inbred mice (Stock No. 005304).
When using the Esr1-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #017911 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Esr1<tm1(cre)And> |
Frozen Mouse Embryo | B6N.129S6(Cg)-Esr1<tm1.1(cre)And>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S6(Cg)-Esr1<tm1.1(cre)And>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S6(Cg)-Esr1<tm1.1(cre)And>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.129S6(Cg)-Esr1<tm1.1(cre)And>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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