Overview

Also Known As:Esr1-Cre

The Esr1^cre knockin allele has Cre recombinase expression directed to estrogen receptor 1 alpha (Esr1 or ERα)-expressing cells by the endogenous Esr1 promoter/enhancer elements; specifically in the ventrolateral subdivision of the ventromedial hypothalamus that, like wildtype mice, is greater in females than males. Expression is also observed in arcuate nucleus (ARH). These Esr1-Cre knockin mice may be useful for studying the function of this ligand-inducible transcription factor in social interactions, neuronal differentiation, sympathetic nervous system development, hypothalamus, female reproductive organs (endometrium and ovarian stroma cells), and male efferent duct epithelial cells. They may also be useful for studying ERα-positive breast cancer and steroid hormone-induced malignancies. Esr1-Cre knockin mice are available on a C57BL/6N background (Stock No. 017911) and a C57BL/6J background (Stock No. 017913).

Donating Investigator

David J Anderson, California Institute of Technology, HHMI

Genetic overview

Genetic Background	Generation
	N3+pN2F8 (2019-11-01 00:00:00)

Esr1^{tm1.1(cre)And}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing, Inducible)	Esr1	estrogen receptor 1 (alpha)

View Genetics

Research Applications

Reproductive Biology Research
Research Tools
Cancer Research
Endocrine Deficiency Research
Cell Biology Research
Neurobiology Research

View All Research Applications

Base Price

Starting at:

$278.00 Domestic price for female

356.51 Domestic price for breeder pair

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Details

Detailed Description

The Esr1^cre (Esr1-Cre) knockin allele harbors an attP-flanked 2A oligopeptide and Cre recombinase gene in the 3' UTR of the estrogen receptor 1 alpha gene (Esr1 or ERα). Under direction of the endogenous Esr1 promoter/enhancer elements, Cre recombinase expression is observed in a pattern similar to that of Esr1. The donating investigator specifically reports Cre recombinase expression in the ventrolateral subdivision of the ventromedial hypothalamus (VMHv1) that, like wildtype mice, is greater in females than males. Expression is also observed in arcuate nucleus (ARH). The donating investigator reports that heterozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In 2014, it is the experience of The Jackson Laboratory Repository colony managers that C57BL/6NJ-congenic Esr1-Cre knockin homozygotes of both sexes do not breed well.

ERα, a nuclear hormone receptor (NHR), binds to steroid hormones in the cytoplasm, causing their translocation into the nucleus and leading to increased transcription of target genes. These Esr1-Cre knockin mice may be useful for studying the function of this ligand-inducible transcription factor in neuronal differentiation, sympathetic nervous system development, hypothalamus, female reproductive organs (endometrium and ovarian stroma cells), and male efferent duct epithelial cells. They may also be useful for studying ERα-positive breast cancer and steroid hormone-induced malignancies.

Development

The Esr1^cre (Esr1-Cre) knockin allele was designed by Dr. David J. Anderson (California Institute of Technology) to insert an attP site, a 2A oligopeptide (mediates ribosomal skipping), the Cre recombinase gene, a frt-flanked PGKneo-polyA cassette, and an attP site within the 3' UTR of the estrogen receptor 1 alpha gene (Esr1). The targeting construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with 129S6/SvEvTac females to establish the colony. Heterozygous mice were backcrossed to C57BL/6N for two generations. Next, a heterozygous male was bred to ACT-FLPe mice (backcrossed onto C57BL/6 background) to remove the frt-flanked PGKneo-polyA cassette. The resulting Esr1-Cre knockin mice were bred to C57BL/6N mice for one more generation (and the ACT-FLPe transgene removed) prior to sending black mutant mice to The Jackson Laboratory Repository in 2012. Upon arrival at The Jackson Laboratory Repository, mutant mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for several generations using a marker-assisted speed congenic approach. As of April 2014, the C57BL/6NJ-congenic Esr1^cre knockin colony has all markers that determine C57BL/6 substrains confirmed to be C57BL/6N allele-type. Overall, at least 148/149 markers (99%) are C57BL/6 allele-type. The single segregating marker is 129 allele-type on chromosome 10 near the targeted locus (consistent with contributions from the ES cell used in creating the targeted mutation).

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase is expressed in the ventrolateral subdivision of the ventromedial hypothalamus, and also in the arcuate nucleus.

Control Suggestions

Wild-type from the colony
005304 C57BL/6NJ

Additional Information

Considerations for Choosing Controls

Selected References

Lee H; Kim DW; Remedios R; Anthony TE; Chang A; Madisen L; Zeng H; Anderson DJ. 2014. Scalable control of mounting and attack by Esr1+ neurons in the ventromedial hypothalamus. Nature 509(7502):627-32PubMed: 24739975 MGI: J:207677

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Genetics

Esr1^{tm1.1(cre)And}

Allele Symbol: Esr1^{tm1.1(cre)And}

Allele Name	targeted mutation 1.1, David J Anderson
Allele Type	Targeted (Recombinase-expressing, Inducible)
Allele Synonym(s)	Esr1^cre; Esr1cre
Gene Symbol and Name	Esr1, estrogen receptor 1 (alpha)
Gene Synonym(s)
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase is expressed in the ventrolateral subdivision of the ventromedial hypothalamus, and also in the arcuate nucleus.
Strain of Origin	129S6/SvEvTac
Chromosome	10
Molecular Note	The Esr1-cre targeting vector was designed to insert an attP site, a 2A oligopeptide (mediates ribosomal skipping), the Cre recombinase gene, a frt-flanked PGKneo-polyA cassette, and an attP site within the 3' UTR of the estrogen receptor 1 alpha gene (Esr1). The targeting construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Chimeric males were bred with 129S6/SvEvTac females to establish the colony. Heterozygotes were bred to ACT-FLPe mice to remove the frt-flanked PGKneo-polyA cassette. Mice were bred to remove the ACT-FLPe transgene from the background.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Reproductive Biology Research
- Endocrine Deficiencies Affecting Gonads
Research Tools
- Endocrine Deficiency Research
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: Cre-lox System
  - Mutagenesis and Transgenesis: transcriptional activation
- Reproductive Biology Research
  - Cre-lox System
- Cre-lox System
  - Cre Recombinase Expression
- Cardiovascular Research
  - Cre-lox System
- Developmental Biology Research
  - Cre-lox System
Cancer Research
- Increased Tumor Incidence
  - Other Tissues/Organs: multiple endocrine tissues (induced)
  - Mammary Gland Tumors
Endocrine Deficiency Research
- Gonad Defects
- Mammary Gland Defects
Cell Biology Research
- Transcriptional Regulation
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Esr1^{tm1.1(cre)And}/Esr1⁺
B6.129S6(Cg)-Esr1

behavior/neurological phenotype

abnormal social investigation
- males injected with an adenoassociated virus (AAV) encoding channelrhodopsin2 show increased numbers and duration of close investigation episodes with weak ChR2 expression or low intensity photostimulation; such conditions also promote mounting behavior towards castrated males or female mice with longer latency than for attack behavior
- mice injected with an AAV encoding NpHR3.0 (inhibitor of Esr1-expressing neuron activity) and photostimulated showed interruption of ongoing close investigation
- owards castrated males or female mice with longer latency than for attack behavior

decreased aggression towards mice
- mice injected with an AAV encoding mCherry only do not show interruption of attack upon photostimulation
- mice injected with an AAV encoding NpHR3.0 (inhibitor of Esr1-expressing neuron activity) and photostimulated at the moment of approach can show either prevention of initiation of attack or interruption of an ongoing attack (sometimes even retreat) while mice injected with an AAV encoding mCherry only do not show interruption of attack upon photostimulation
- photostimulation does not elicit aggression in males injected with an AAV (adenoassociated virus) in which cre activity results in deletion of the channelrhodopsin2 sequence; AAV-injected control males show attack behavior upon photostimulation

increased aggression towards mice
- photostimulation of Esr1 expressing neurons in the ventrolateral subdivision of the ventromedial hypothalamus (VMHvl) in males injected with a cre-activated adenoassociated virus (AAV) with a floxed channelrhodopsin 2/mCherry (ChR2/mCherry) cassette in reverse orientation results in intense attack towards female and castrated male intruders (cre expression results in inversion of the ChR2 cassette followed by expression in Esr1 positive neurons)
- verse orientation results in intense attack towards female and castrated male intruders (cre expression results in inversion of the ChR2 cassette followed by expression in Esr1 positive neurons)

References

Lee H; Kim DW; Remedios R; Anthony TE; Chang A; Madisen L; Zeng H; Anderson DJ. 2014. Scalable control of mounting and attack by Esr1+ neurons in the ventromedial hypothalamus. Nature 509(7502):627-32PubMed: 24739975 MGI: J:207677

Additional - Esr1^{tm1.1(cre)And} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Generic Cre Probe
- Separated PCR:Esr1
- Standard PCR:Esr1
- Genotyping resources and troubleshooting
Breeding Considerations

In 2014, it is the experience of The Jackson Laboratory Repository colony managers that C57BL/6NJ-congenic Esr1-Cre knockin homozygotes of both sexes do not breed well.

Therefore when maintaining our live colony, heterozygous mice may be bred with wildtype mice from the colony or with C57BL/6NJ inbred mice (Stock No. 005304).
- Additional Breeding and Husbandry Support
Mating System
- Wild-type x Heterozygote
- Heterozygote x Wild-type
Citation
When using the Esr1-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #017911 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX9 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or wildtype for Esr1<tm1(cre)And>

Related Products and Services

Frozen Mouse Embryo	B6N.129S6(Cg)-Esr1<tm1.1(cre)And>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6N.129S6(Cg)-Esr1<tm1.1(cre)And>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6N.129S6(Cg)-Esr1<tm1.1(cre)And>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6N.129S6(Cg)-Esr1<tm1.1(cre)And>/J Frozen Embryo	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:Esr1-Cre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Esr1tm1.1(cre)And

Allele Symbol: Esr1tm1.1(cre)And

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Esr1tm1.1(cre)And/Esr1+B6.129S6(Cg)-Esr1

behavior/neurological phenotype

References

Additional - Esr1tm1.1(cre)And related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Esr1^{tm1.1(cre)And}

Allele Symbol: Esr1^{tm1.1(cre)And}

Genotype: Esr1^{tm1.1(cre)And}/Esr1⁺
B6.129S6(Cg)-Esr1

Additional - Esr1^{tm1.1(cre)And} related