BTBR6 transgenic mice have a bidirectional tetracycline-responsive promoter (TRE; tetO) driving expression of two individual fusion genes; the halorhodopsin/EGFP fusion gene (HaloR-EGFP) and channelrhodopsin-2/mCherry fusion gene (ChR2-mCherry). When bred to other mice expressing tTA or reverse tetracycline-controlled transactivator protein (rtTA), simultaneous HaloR-EGFP and ChR2-mCherry expression in the resulting double mutant offspring can be regulated by tetracycline or its analog doxycycline (dox). The specificity of the tTA strain chosen to generate the bi-transgenic line confers inducible conditional expression of the transgene, and via photostimulation expression of Channelrhodopsin-2, in defined cell populations.
When crossed with a Camk2a-tTA strain (see for example, Stock No. 003010), double transgenic mice show specific expression of EGFP and mCherry in the dorsal striatum and projections into the globus pallidus and substantia nigra, allowing for visualization of neuronal excitability and neural circuits.

Development

A transgenic construct containing a SV40 polyadenylation site sequence (reverse orientation) followed by the halorhodopsin-enhanced green fluorescent protein fusion gene cDNA, and the Channelrhodopsin-2 (Chlamydomonas reinhardtii)/mCherry, Red Fluorescent Protein fusion gene cDNA followed by a SV40 polyadenylation site sequence, under the control of the a bidirectional tetracycline-responsive promoter (TRE; tetO) was injected into fertilized CBA;B6 mouse eggs. Founder line 6 was subsequently established. The mice were then crossed to mice on the B6;129 background for several generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Expression Data

Expressed Gene	COP4, Channelrhodopsin, Chlamydomonas
Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	HOP, Halorhodopsin, Natronomonas
Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of HOP/EGFP,COP4/mCherry may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. The specificity of the tTA strain chosen to generate the bi-transgenic line confers inducible conditional expression of the transgene, and via photostimulation expression of Channelrhodopsin-2, in defined cell populations. For example, when crossed with a Camk2a-tTA strain, double transgenic mice show specific expression of EGFP and mCherry in the dorsal striatum and projections into the globus pallidus and substantia nigra.

Control Suggestions

Noncarrier

Additional Information

Considerations for Choosing Controls

Selected References

Chuhma N; Tanaka KF; Hen R; Rayport S. 2011. Functional connectome of the striatal medium spiny neuron. J Neurosci 31(4):1183-92. PubMed: 21273403 MGI: J:188102

View All References

Genetics

Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk

Allele Symbol: Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk

Allele Name	transgene insertion 6, Kenji Tanaka
Allele Type	Transgenic (Inducible, Reporter)
Allele Synonym(s)	BTR6; Tg(tetO-HOP/EGFP,-COP4/mCherry)6Sray
Gene Symbol and Name	Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk, transgene insertion 6, Kenji Tanaka
Gene Synonym(s)	Tg(tetO-HOP/EGFP,-COP4/mCherry)6Sray
Promoter	tetO, tet operator,
Expressed Gene	COP4, Channelrhodopsin, Chlamydomonas
Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	HOP, Halorhodopsin, Natronomonas
Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of HOP/EGFP,COP4/mCherry may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. The specificity of the tTA strain chosen to generate the bi-transgenic line confers inducible conditional expression of the transgene, and via photostimulation expression of Channelrhodopsin-2, in defined cell populations. For example, when crossed with a Camk2a-tTA strain, double transgenic mice show specific expression of EGFP and mCherry in the dorsal striatum and projections into the globus pallidus and substantia nigra.
Strain of Origin	CBA x C57BL/6
Chromosome	UN
Molecular Note	A bidirectional tetracycline responsive element drives inducible expression of a halorhodopsin/EGFP fusion and a channelrhodopsin-2/m-Cherry fusion. Line 6 was the only line to exhibit expression.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Fluorescent protein expression in neural tissue
- Optogenetic and Chemogenetic tools
  - Channelrhodopsin expressing strains
- Tet Expression System
  - tTA/rtTA Responsive Strains
Research Tools
- Fluorescent Proteins
- Genetics Research
  - Tissue/Cell Markers
- Neurobiology Research
  - cell marker
- Tet Expression Systems
  - tTA/rtTA Responsive Strains

Genotype: GFP related

Research Tools
- Fluorescent Proteins

References

Chuhma N; Tanaka KF; Hen R; Rayport S. 2011. Functional connectome of the striatal medium spiny neuron. J Neurosci 31(4):1183-92. PubMed: 21273403 MGI: J:188102

Additional - Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk related

Tanaka KF; Matsui K; Sasaki T; Sano H; Sugio S; Fan K; Hen R; Nakai J; Yanagawa Y; Hasuwa H; Okabe M; Deisseroth K; Ikenaka K; Yamanaka A. 2012. Expanding the repertoire of optogenetically targeted cells with an enhanced gene expression system. Cell Rep 2(2):397-406. PubMed: 22854021 MGI: J:186771

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Hemizygous or Non Carrier for Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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$2,595.00 per straw or vial

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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