| Allele Name | transgene insertion 6, Kenji Tanaka |
| Allele Type | Transgenic (Reporter, Inducible) |
| Allele Synonym(s) | BTR6; Tg(tetO-HOP/EGFP,-COP4/mCherry)6Sray |
| Gene Symbol and Name | Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk, transgene insertion 6, Kenji Tanaka |
| Gene Synonym(s) | |
| Promoter | tetO, tet operator, |
|---|
| Expressed Gene | GFP, Green Fluorescent Protein, |
|---|
| Expressed Gene | RFP, Red Fluorescent Protein, coral |
|---|
| Expressed Gene | COP4, Channelrhodopsin, Chlamydomonas |
|---|
| Expressed Gene | HOP, Halorhodopsin, Natronomonas |
|---|
| Site of Expression | When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of HOP/EGFP,COP4/mCherry may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. The specificity of the tTA strain chosen to generate the bi-transgenic line confers inducible conditional expression of the transgene, and via photostimulation expression of Channelrhodopsin-2, in defined cell populations.
For example, when crossed with a Camk2a-tTA strain, double transgenic mice show specific expression of EGFP and mCherry in the dorsal striatum and projections into the globus pallidus and substantia nigra. |
|---|
| Strain of Origin | CBA x C57BL/6 |
| Chromosome | UN |
| Molecular Note | A bidirectional tetracycline responsive element drives inducible expression of a halorhodopsin/EGFP fusion and a channelrhodopsin-2/m-Cherry fusion. Line 6 was the only line to exhibit expression. |
