These FAT3 mutant mice possess loxP sites flanking exon 23 (transmembrane domain) of the FAT tumor suppressor homolog 3 gene, and may be useful for studying dendrite development.
Micheal Deans, Johns Hopkins Univ. School of Medicine
FAT3, an atypical cadherin protein, is expressed on amacrine cell (AC) processes in the developing and mature inner plexiform layer (IPL) of the retina and is associated with dendrite morphogenesis. These mutant mice possess loxP sites flanking exon 23 of the Fat3 gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 23 deleted in the cre-expressing tissues. This strain may be useful for studying dendrite development.
A targeting vector was designed to insert a loxP site upstream of exon 23, which contains the transmembrane domain, followed by a frt-flanked neomycin resistance (neo) cassette, and a second loxP site upstream of exon 24. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred. Offspring were bred with Tg(ACTFLPe)9205Dym transgenic mice on a C57BL/6 congenic background to delete the neo cassette. The resulting progeny were crossed to remove the Flp-expressing transgene. Mice were maintained as a backcross to B6129PF1/J. Upon arrival at The Jackson Laboratory, mice were bred to B6129PF1/J mice (100492) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1.1, Lisa Goodrich|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Fat3, FAT atypical cadherin 3|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A loxP site was inserted upstream of exon 23. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 23. Flp-mediated recombination removed the neo cassette and left exon 23 floxed.|
|Mutations Made By|| |
Lisa Goodrich, Harvard Medical School
While maintaining a live colony, these mice are bred as homozygotes.
When using the B6;129P-Fat3tm1.1Good/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017839 in your Materials and Methods section.