A neomycin resistance (neo) cassette replaces the entire coding region of the RAS-related C3 botulinium substrate 3 (Rac3) gene, abolishing gene function. RAC3 is a GTP-binding protein which plays a role in the regulation of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Homozygotes are viable and fertile with no overt phenotype. Mice lacking Rac3 have increased motor coordination and learning on the rotarod test but reduced behavioral flexibility. When these RAC3 null mice are bred to BCR/ABL Tg mice (Stock No. 017833) the resulting female offspring show a delay in the development of Philadelphia chromosome positive (Ph+) acute lymphoblastic leukemia.
A targeting vector was designed to replace the entire coding region of the RAS-related C3 botulinium substrate 3 (Rac3) gene with a neomycin resistance (neo) cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6 females. These mice were backcrossed 12 generations to C57BL/6J background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Nora C Hesterkamp|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Rac3, Rac family small GTPase 3|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||The entire coding region was replaced with a PGK-NEO cassette. QRT-PCR confirmed the absence of gene products.|
|Mutations Made By|| |
Nora Heisterkamp, Childrens Hospital Los Angeles
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129-Rac3tm1Hkp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017832 in your Materials and Methods section.