Overview

Also Known As:CRAMP KO

These Camp (formerly Cnlp) knock-out mice are more susceptible to bacterial infections and may be useful in studies of innate immune response, regulation of immune response, wound healing/angiogenesis and tumor development.

Donating Investigator

Richard L. Gallo, University of California at San Diego

Genetic overview

Genetic Background	Generation
	N7pN2F7 (2019-11-01 00:00:00)

Camp^tm1Rlg

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Camp	cathelicidin antimicrobial peptide

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Research Applications

Research Tools
Internal/Organ Research
Cancer Research
Immunology, Inflammation and Autoimmunity Research

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Base Price

Starting at:

$255.00 Domestic price for female

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Details

Detailed Description

The mouse Camp gene is an ortholog of the human gene CAMP, which encodes the precursor of cathelicidin antimicrobial peptide LL-37 (or CRAMP in mouse). Expressed mucosal epithelial cells, circulating neutrophils, and myeloid bone marrow cells, Camp is an essential part of the first line of defense against infection. In addition to antimicrobial activity, cathelicidin antimicrobial peptide plays a role in NK cell-mediated tumor growth suppression, and when secreted by neutrophils acts, as an attractant for monocytes, promoting wound healing or angiogenesis. Mouse CRAMP is implicated in adaptive immune response regulation and can interfere with TLR function via interactions with hyaluronan. Mice deficient in CRAMP are more susceptible to experimentally induced necrotic skin infection with Group A Streptococcus, urinary tract infection with uropathogenic E. coli, Pseudomonas aeruginosa infection, and meningococcal Neisseria meningitidis infection. Skin lesions, due to Group A Streptococcus infection, are larger and persist longer in homozygotes when compared to controls. Heterozygotes exhibit an intermediate Group A Streptococcus bacterial susceptibility phenotype. Within 1 hour of infection, more uropathogenic E. coli bacteria are attached to urinary bladder mucosa of mutant mice. 48 hours after infection, the number of bacteria in the urinary tract of homozygous mice is higher than wildtype controls. Mutant mice challenged with uropathogenic E. coli developed more severe urinary tract infections and exhibit higher mortality from septicemia when compared to controls. After intranasal Pseudomonas
aeruginosa inoculation, CRAMP deficient mice exhibit significantly higher levels of bacterial CFUs compared to controls. When pretreated with flagellin, only one third of infected CRAMP deficient mice survived, compared to complete survival of all infected wildtype mice. Homozygotes also develop more severe infection of Pseudomonas aeruginosa in induced corneal keratitis. An increased invasion rate is observed after oral administration of Listeria monocytogenes to homozygotes. In an experimentally induced skin inflammation model for atopic dermatitis, homozygotes exhibit enhanced swelling and more severe epidermal hypertrophy. The epidermal permeability barrier is functionally impaired in homozygotes. Induced irritation of the skin of homozygous mice by stratum corneum tryptic enzyme or physical abrastion results in reduced inflammatory infiltrate, when compared to controls. In vaccinia virus skin infection (eczema vaccinatum), homozygotes on the BALB/c background have higher levels of virus replication in skin biopsies compared to controls. Knock out mice are more susceptible to meningococcal Neisseria meningitidis infection with higher CFU load and mortality rate. When B and T cells from homozygotes are cultured in Th2-inducing conditions, the mutant B cells produce less IgG1 and IgE, and a higher ratio of mutant IL-4+ T cells is observed, when compared to cultures isolated from wildtype mice. Xenograft tumors growth is accelerated in homozygotes: B16.F10 melanoma tumors grow faster and obtain a larger size, and RMA-S lymphoma cell tumor development is earlier when compared to controls. NK cells isolated from homozygotes display defective cytotoxic activity. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern blot analysis of bone marrow or by Western blot analysis of epidermis from homozygotes.

Development

A targeting vector containing a PGK-neo cassette was used to disrupt exons 3 and 4. The construct was electroporated into unspecified 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to 129/SvJ female mice, and then backcrossed to C57BL/6J for seven generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Nizet V; Ohtake T; Lauth X; Trowbridge J; Rudisill J; Dorschner RA; Pestonjamasp V; Piraino J; Huttner K; Gallo RL. 2001. Innate antimicrobial peptide protects the skin from invasive bacterial infection. Nature 414(6862):454-7PubMed: 11719807 MGI: J:72924

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Genetics

Camp^tm1Rlg

Allele Symbol: Camp^tm1Rlg

Allele Name	targeted mutation 1, Richard L Gallo
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Camp^-; Cnlp-; Cnlp-null; Cramp-ko
Gene Symbol and Name	Camp, cathelicidin antimicrobial peptide
Gene Synonym(s)
Strain of Origin	129X1/SvJ
Chromosome	9
Molecular Note	Exons 3 and 4, which encode the entire mature domain of the protein, were replaced with a PGK-neo cassette via homologous recombination. Northern blot analysis using a probe to exon 4 confirmed the absence of gene expression in bone marrow of homozygous mutant animals.
Mutations Made By	Carlos Aguilera, University of California at San Diego

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - genes regulating susceptibility to infectious disease and endotoxin
- Cancer Research
  - B, T, and NK cell deficiency, xenograft/transplant host
  - tumor immunology
Internal/Organ Research
- Wound Healing
Cancer Research
- Genes Regulating Growth and Proliferation
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Camp^tm1Rlg/Camp^tm1Rlg
involves: 129X1/SvJ * C57BL/6

hematopoietic system phenotype

increased neutrophil cell number
- in the cornea of mice infected with F. solani

immune system phenotype

increased incidence of corneal inflammation
- in mice infected with F. solani

increased neutrophil cell number
- in the cornea of mice infected with F. solani

increased susceptibility to bacterial infection
- homozygotes are more susceptible to Group A Streptococcus infection, developing larger areas of infection and showing a more rapid increase in lesion areas and longer persistence of lesions

increased susceptibility to fungal infection
- mice infected with F. solani exhibit more rapid development of keratitis (cornea cloudiness and edema), increased neutrophil infiltration (determined by MPO activity and plate count) and enhanced fungal load without recovery compared with wild-type mice

vision/eye phenotype

increased incidence of corneal inflammation
- in mice infected with F. solani

Genotype: Camp^tm1Rlg/Camp⁺
involves: 129X1/SvJ * C57BL/6

immune system phenotype

increased susceptibility to bacterial infection
- heterozygotes are more susceptible to Group A Streptococcus infection, developing intermediate sized lesions

Genotype: Camp^tm1Rlg/Camp^tm1Rlg
129X1(B6)-Camp/Kini

immune system phenotype

increased susceptibility to bacterial infection
- homozygotes with urinary tract infection exhibit higher number of bacteria in kidneys and larger size of kidneys and have more severe systemic signs of infection such as weight loss and increased mortality
- increased susceptibility to uropathogenic E. coli infection; exhibit a higher infection rate (increased presence of bacteria in urinary tract) and higher numbers of bacteria attached to bladders and surviving on epithelial cells after 30 min

increased susceptibility to bacterial infection induced morbidity/mortality
- homozygotes with urinary tract infection (caused by E.coli infection) show increased mortality, with 3 of 18 dying from septicemia compared to none of wild-type

mortality/aging

increased susceptibility to bacterial infection induced morbidity/mortality
- homozygotes with urinary tract infection (caused by E.coli infection) show increased mortality, with 3 of 18 dying from septicemia compared to none of wild-type

References

Nizet V; Ohtake T; Lauth X; Trowbridge J; Rudisill J; Dorschner RA; Pestonjamasp V; Piraino J; Huttner K; Gallo RL. 2001. Innate antimicrobial peptide protects the skin from invasive bacterial infection. Nature 414(6862):454-7PubMed: 11719807 MGI: J:72924

Additional - Camp^tm1Rlg related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Camp Alternate2
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the CRAMP KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #017799 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX9 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Not-For-Profit & Academic Pricing

Service/Product	Description	Price
	Heterozygous for Camp<tm1Rlg>

Related Products and Services

Frozen Mouse Embryo	B6.129X1-Camp<tm1Rlg>/J	$2595.00
Frozen Mouse Embryo	B6.129X1-Camp<tm1Rlg>/J	$2595.00
Frozen Mouse Embryo	B6.129X1-Camp<tm1Rlg>/J	$3373.50
Frozen Mouse Embryo	B6.129X1-Camp<tm1Rlg>/J	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

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Licensing Information

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Also Known As:CRAMP KO

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Camptm1Rlg

Allele Symbol: Camptm1Rlg

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Camptm1Rlg/Camptm1Rlginvolves: 129X1/SvJ * C57BL/6

hematopoietic system phenotype

immune system phenotype

vision/eye phenotype

Genotype: Camptm1Rlg/Camp+involves: 129X1/SvJ * C57BL/6

immune system phenotype

Genotype: Camptm1Rlg/Camptm1Rlg129X1(B6)-Camp/Kini

immune system phenotype

mortality/aging

References

Additional - Camptm1Rlg related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Not-For-Profit & Academic Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Camp^tm1Rlg

Allele Symbol: Camp^tm1Rlg

Genotype: Camp^tm1Rlg/Camp^tm1Rlg
involves: 129X1/SvJ * C57BL/6

Genotype: Camp^tm1Rlg/Camp⁺
involves: 129X1/SvJ * C57BL/6

Genotype: Camp^tm1Rlg/Camp^tm1Rlg
129X1(B6)-Camp/Kini

Additional - Camp^tm1Rlg related