In this knockout strain, exon 2 of the Fcer1g gene is disrupted. These mice develop mild or no symptoms in response to a collagen-induced arthritis challenge. These mice may have applications in studies related to autoimmunity and inflammation.
Sandra Kleinau, Biomedical Center, Uppsala University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Fcer1g | Fc receptor, IgE, high affinity I, gamma polypeptide |
Fc IgE receptors are found on immune effector cells and mediate macrophage phagocytosis, antibody-dependent cell-mediated cytotoxicity, mast cell degranulation, IgE-mediated systemic anaphylaxis and neutrophil recruitment.
These mice carry a targeted mutation of the Fcer1g (Fc receptor, IgE, high affinity I, gamma polypeptide ) gene and have been backcrossed onto the autoimmune-susceptible DBA/1 background. In homozygous mice challenged with collagen-induced arthritis, fewer mice develop arthritic symptoms, and the symptoms are mild compared to wildtype controls. Mutant mice that develop collagen-induced arthritis do not exhibit inflammatory cell infiltration in the synovium or cartilage and bone erosion. However, homozygotes produce total IgG anti-collagen antibodies at levels similar to those seen in controls. During backcrossing, the Y chromosome may not have been fixed to the DBA/1 genetic background. Mice that are homozygous for the targeted mutation are viable, fertile and normal in size.
A targeting vector containing a NEO cassette was used to disrupt exon 2, which created a new stop codon 239 bp downstream of the integration site. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to DBA/1 for 14 generations.
Upon arrival at The Jackson Laboratory, the mice were crossed to DBA/1J (Stock No. 000670) at least once to establish the colony.
Allele Name | targeted mutation 1, Jeffrey V Ravetch |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Fcepsilonrgamma-; Fcer1gtm1; Fcer1gtm1Ra; Fcerg1tm1; FcgammaR-; FcR-; FcR gamma-; FcRg-; FcRgamma-; FcRgammanl; FcRgammac-; FcRgamma-chain-; FcRKO; gamma- |
Gene Symbol and Name | Fcer1g, Fc receptor, IgE, high affinity I, gamma polypeptide |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 1 |
Molecular Note | A neomycin selection cassette was inserted into exon 2, creating a new stop codon 239 bp downstream of the integration site. RT-PCR analysis on RNA isolated from activated macrophages, mast cells and NK cells of homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on lysates derived from activated macrophages, mast cells and NK cells of homozygous mice confirmed that no protein is encoded by this allele. |
Mutations Made By | Dr. Jeffrey Ravetch, Memorial Sloan Kettering Cancer Center |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the FcRg- mouse strain in a publication, please cite the originating article(s) and include JAX stock #017793 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Fcer1g<tm1Rav> |
Frozen Mouse Embryo | D1.129P2(B6)-Fcer1g<tm1Rav>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | D1.129P2(B6)-Fcer1g<tm1Rav>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | D1.129P2(B6)-Fcer1g<tm1Rav>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | D1.129P2(B6)-Fcer1g<tm1Rav>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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