Overview

Also Known As: FcRg-

In this knockout strain, exon 2 of the Fcer1g gene is disrupted. These mice develop mild or no symptoms in response to a collagen-induced arthritis challenge. These mice may have applications in studies related to autoimmunity and inflammation.

Donating Investigator

Sandra Kleinau, Biomedical Center, Uppsala University

Genetic overview

Genetic Background	Generation

Fcer1g^tm1Rav

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Fcer1g	Fc receptor, IgE, high affinity I, gamma polypeptide

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Research Applications

Immunology, Inflammation and Autoimmunity Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Fc IgE receptors are found on immune effector cells and mediate macrophage phagocytosis, antibody-dependent cell-mediated cytotoxicity, mast cell degranulation, IgE-mediated systemic anaphylaxis and neutrophil recruitment.
These mice carry a targeted mutation of the Fcer1g (Fc receptor, IgE, high affinity I, gamma polypeptide ) gene and have been backcrossed onto the autoimmune-susceptible DBA/1 background. In homozygous mice challenged with collagen-induced arthritis, fewer mice develop arthritic symptoms, and the symptoms are mild compared to wildtype controls. Mutant mice that develop collagen-induced arthritis do not exhibit inflammatory cell infiltration in the synovium or cartilage and bone erosion. However, homozygotes produce total IgG anti-collagen antibodies at levels similar to those seen in controls. During backcrossing, the Y chromosome may not have been fixed to the DBA/1 genetic background. Mice that are homozygous for the targeted mutation are viable, fertile and normal in size.

Development

A targeting vector containing a NEO cassette was used to disrupt exon 2, which created a new stop codon 239 bp downstream of the integration site. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to DBA/1 for 14 generations.
Upon arrival at The Jackson Laboratory, the mice were crossed to DBA/1J (Stock No. 000670) at least once to establish the colony.

Control Suggestions

000670 DBA/1J

Additional Information

Considerations for Choosing Controls

Selected References

Takai T; Li M; Sylvestre D; Clynes R; Ravetch JV. 1994. FcR gamma chain deletion results in pleiotrophic effector cell defects. Cell 76(3):519-29PubMed: 8313472 MGI: J:39248

View All References

Genetics

Fcer1g^tm1Rav

Allele Symbol: Fcer1g^tm1Rav

Allele Name	targeted mutation 1, Jeffrey V Ravetch
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	targeted mutation 1, Jeffrey V Ravetch; Fcer1g^tm1Rav
Gene Symbol and Name	Fcer1g, Fc receptor, IgE, high affinity I, gamma polypeptide
Gene Synonym(s)	Fce1g; FcepsilonRI; FcR-gamma; FcR[g]; FcRgamma; Ly-50; FCRG; AI573376; Fce1g; Ly-50; lymphocyte antigen 50; expressed sequence AI573376; Fc epsilon high affinity receptor gamma
Strain of Origin	129P2/OlaHsd
Chromosome	1
Molecular Note	A neomycin selection cassette was inserted into exon 2, creating a new stop codon 239 bp downstream of the integration site. RT-PCR analysis on RNA isolated from activated macrophages, mast cells and NK cells of homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on lysates derived from activated macrophages, mast cells and NK cells of homozygous mice confirmed that no protein is encoded by this allele.
Mutations Made By	Dr. Jeffrey Ravetch, Memorial Sloan Kettering Cancer Center

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- Autoimmunity
  - collagen induced arthritis
  - experimentally induced rheumatoid arthritis
- Inflammation
  - Neutrophil defects
  - collagen induced arthritis

Genotype: Fcer1g^tm1Rav related

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
- Inflammation

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Fcer1g^tm1Rav/Fcer1g^tm1Rav
involves: 129P2/OlaHsd * C57BL/6

cardiovascular system phenotype

decreased myocardial infarction size
- fewer platelet microthrombi and neutrophils in the capillaries of the myocardium
- infarct size after coronary occlusion/reperfusion is significantly reduced
- occluded microthrombi in 12% of capillaries as compared to 38% in controls

homeostasis/metabolism phenotype

abnormal platelet activation
- platelet aggregates display reduced annexinV positivity after perfusion unlike wild-type platelets
- platelet calcium response fluctuates and is lower than in wild-type platelets
- while platelets exhibit activation at high shear stress, they form looser aggregates and disintegrate more frequently than wild-type platelets

decreased myocardial infarction size
- fewer platelet microthrombi and neutrophils in the capillaries of the myocardium
- infarct size after coronary occlusion/reperfusion is significantly reduced
- occluded microthrombi in 12% of capillaries as compared to 38% in controls

decreased platelet aggregation
- reduced aggregation in response to collagen

decreased platelet calcium level

immune system phenotype

abnormal dendritic cell antigen presentation
- deficient antigen presentation by bone marrow dendritic cells

abnormal immune cell physiology

abnormal immunoglobulin level
- serum IgG response to OVA challenge of sensitized mice more moderate than in controls (37% increase as opposed to 260%)

abnormal mast cell physiology
- degranulation and serotonin release largely suppressed
- IgG1 binding normal
- loss of IgE and IgG2a binding
- significantly reduced prostaglandin D2 release

abnormal NK cell physiology
- antibody dependent, cell-mediated cytotoxicity almost totally lost

decreased susceptibility to experimental autoimmune encephalomyelitis
- completely protected against anaphylaxis from a second dose of myelin oligodendrocyte glycoprotein
- considerably reduced when induced with myelin oligodendrocyte glycoprotein
- fewer inflammatory foci in the central nervous system
- more frequent disease remission

decreased susceptibility to type I hypersensitivity reaction
- IgG1 dependent passive systemic anaphylaxis does not occur
- sensitized mice survive OVA challenge whereas controls die within 20 minutes
- unable to mount a passive cutaneous anaphylactic response

impaired macrophage phagocytosis
- phagocytosis blocked regardless of Ig binding

increased IgE level
- serum IgE response to OVA challenge of sensitized mice greater than that of controls

cellular phenotype

impaired macrophage phagocytosis
- phagocytosis blocked regardless of Ig binding

mammalian phenotype

immune system phenotype
- the development of NK T cells is normal in these mice

skeleton phenotype
- Normal - mice exhibit normal bone volume and osteoclast function

hematopoietic system phenotype

abnormal immunoglobulin level
- serum IgG response to OVA challenge of sensitized mice more moderate than in controls (37% increase as opposed to 260%)

abnormal mast cell physiology
- degranulation and serotonin release largely suppressed
- IgG1 binding normal
- loss of IgE and IgG2a binding
- significantly reduced prostaglandin D2 release

abnormal NK cell physiology
- antibody dependent, cell-mediated cytotoxicity almost totally lost

abnormal platelet activation
- platelet aggregates display reduced annexinV positivity after perfusion unlike wild-type platelets
- platelet calcium response fluctuates and is lower than in wild-type platelets
- while platelets exhibit activation at high shear stress, they form looser aggregates and disintegrate more frequently than wild-type platelets

decreased platelet aggregation
- reduced aggregation in response to collagen

decreased platelet calcium level

impaired macrophage phagocytosis
- phagocytosis blocked regardless of Ig binding

increased IgE level
- serum IgE response to OVA challenge of sensitized mice greater than that of controls

Genotype: Fcer1g^tm1Rav/Fcer1g^tm1Rav
B6.129P2-Fcer1g

immune system phenotype

decreased interleukin-1 beta secretion
- by BMDCs in response to water-soluble mannans from Serotype A C. albicans
- by BMDCs stimulated by yeast or hyphae forms of C.albicans

decreased interleukin-10 secretion
- by BMDCs in response to Mannans and water-soluble mannans from Serotype A C. albicans
- by BMDCs stimulated by yeast or hyphae forms of C.albicans

decreased interleukin-12b secretion
- by BMDCs in response to Mannans and water-soluble mannans from Serotype A C. albicans
- by BMDCs stimulated by yeast or hyphae forms of C.albicans

decreased interleukin-23 secretion
- by BMDCs stimulated by yeast form of C.albicans

decreased interleukin-6 secretion
- by BMDCs in response to Mannans and water-soluble mannans from Serotype A C. albicans
- by BMDCs stimulated by yeast or hyphae forms of C.albicans

decreased tumor necrosis factor secretion
- by BMDCs in response to Mannans and water-soluble mannans from Serotype A C. albicans
- by BMDCs stimulated by yeast or hyphae forms of C.albicans

Genotype: Fcer1g^tm1Rav/Fcer1g^tm1Rav
involves: 129P2/OlaHsd

cardiovascular system phenotype

hematoma
- greatly enlarged area of experimentally induced hematomas

hematopoietic system phenotype

abnormal hemoglobin
- increased hemisphere hemoglobin content in mouse brains injected with phosphate buffered saline

decreased platelet aggregation
- mutants exhibit a lack of collagen-induced aggregation of platelets

homeostasis/metabolism phenotype

decreased platelet aggregation
- mutants exhibit a lack of collagen-induced aggregation of platelets

immune system phenotype

increased susceptibility to parasitic infection
- after second infestation to ticks, mice fail to exhibit resistance (repletion) unlike similarly treated wild-type mice
- Normal - however, transfer of bone marrow from Kit^W-sh homozygotes restores repletion

mammalian phenotype

skeleton phenotype
- Normal - no effect on osteoclast size and number in tibias

References

Takai T; Li M; Sylvestre D; Clynes R; Ravetch JV. 1994. FcR gamma chain deletion results in pleiotrophic effector cell defects. Cell 76(3):519-29PubMed: 8313472 MGI: J:39248

Additional - Fcer1g^tm1Rav related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Fcer1g^tm1Rav
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the FcRg- mouse strain in a publication, please cite the originating article(s) and include JAX stock #017793 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Fcer1g<tm1Rav>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	D1.129P2(B6)-Fcer1g<tm1Rav>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	D1.129P2(B6)-Fcer1g<tm1Rav>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	D1.129P2(B6)-Fcer1g<tm1Rav>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	D1.129P2(B6)-Fcer1g<tm1Rav>/J Frozen Embryo	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Also Known As: FcRg-

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Fcer1gtm1Rav

Allele Symbol: Fcer1gtm1Rav

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: Fcer1gtm1Rav related

Mammalian Phenotype Terms by Genotype

Genotype: Fcer1gtm1Rav/Fcer1gtm1Ravinvolves: 129P2/OlaHsd * C57BL/6

cardiovascular system phenotype

homeostasis/metabolism phenotype

immune system phenotype

cellular phenotype

mammalian phenotype

hematopoietic system phenotype

Genotype: Fcer1gtm1Rav/Fcer1gtm1RavB6.129P2-Fcer1g

immune system phenotype

Genotype: Fcer1gtm1Rav/Fcer1gtm1Ravinvolves: 129P2/OlaHsd

cardiovascular system phenotype

hematopoietic system phenotype

homeostasis/metabolism phenotype

immune system phenotype

mammalian phenotype

References

Additional - Fcer1gtm1Rav related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Fcer1g^tm1Rav

Allele Symbol: Fcer1g^tm1Rav

Genotype: Fcer1g^tm1Rav related

Genotype: Fcer1g^tm1Rav/Fcer1g^tm1Rav
involves: 129P2/OlaHsd * C57BL/6

Genotype: Fcer1g^tm1Rav/Fcer1g^tm1Rav
B6.129P2-Fcer1g

Genotype: Fcer1g^tm1Rav/Fcer1g^tm1Rav
involves: 129P2/OlaHsd

Additional - Fcer1g^tm1Rav related