D1.129P2(B6)-Fcer1g^tm1Rav/J

Stock number: 017793
Product name: FcRg-
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

In this knockout strain, exon 2 of the Fcer1g gene is disrupted. These mice develop mild or no symptoms in response to a collagen-induced arthritis challenge. These mice may have applications in studies related to autoimmunity and inflammation.

Detailed description

Fc IgE receptors are found on immune effector cells and mediate macrophage phagocytosis, antibody-dependent cell-mediated cytotoxicity, mast cell degranulation, IgE-mediated systemic anaphylaxis and neutrophil recruitment. These mice carry a targeted mutation of the Fcer1g (Fc receptor, IgE, high affinity I, gamma polypeptide ) gene and have been backcrossed onto the autoimmune-susceptible DBA/1 background. In homozygous mice challenged with collagen-induced arthritis, fewer mice develop arthritic symptoms, and the symptoms are mild compared to wildtype controls. Mutant mice that develop collagen-induced arthritis do not exhibit inflammatory cell infiltration in the synovium or cartilage and bone erosion. However, homozygotes produce total IgG anti-collagen antibodies at levels similar to those seen in controls. During backcrossing, the Y chromosome may not have been fixed to the DBA/1 genetic background. Mice that are homozygous for the targeted mutation are viable, fertile and normal in size.

Development

A targeting vector containing a NEO cassette was used to disrupt exon 2, which created a new stop codon 239 bp downstream of the integration site. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to DBA/1 for 14 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to DBA/1J (Stock No. 000670) at least once to establish the colony.

Allele

Symbol: Fcer1g^tm1Rav
Name: targeted mutation 1, Jeffrey V Ravetch
MGI accession ID: MGI:1857165
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Fcer1g
Marker name: Fc receptor, IgE, high affinity I, gamma polypeptide
Chromosome: 1
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin selection cassette was inserted into exon 2, creating a new stop codon 239 bp downstream of the integration site. RT-PCR analysis on RNA isolated from activated macrophages, mast cells and NK cells of homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on lysates derived from activated macrophages, mast cells and NK cells of homozygous mice confirmed that no protein is encoded by this allele.