129S-Slc40a1^tm2Nca/J

Stock number: 017790
Product name: Ferroportin^flox
Research areas: Hematological Research, Metabolism Research, Research Tools

Description

These ferroportin^fl mice possess loxP sites flanking exons 6 and 7 of the solute carrier family 40 (iron-regulated transporter), member 1 (Slc40a1) gene and have applications in studies related to type 4 hemochromatosis, anemia, and iron homeostasis.

Detailed description

Ferroportin is the only known iron exporter and is essential for iron homeostasis and iron absorption in the intestine. Mutations in the human ferroportin gene, SLC40A1 (solute carrier family 40 (iron-regulated transporter), member 1), can cause type 4 hemochromatosis. These mice possess loxP sites on either side of exons 6 and 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 and 7 deleted in the cre-expressing tissues.

When bred to a strain with inducible Cre recombinase expression in the intestine, this mutant mouse strain may be useful in studies of intestinal iron absorption and anemia.

When bred to a strain expressing Cre recombinase in epiblast derived tissues (Meox2^tm1(cre)Sor; see Stock No. 003755 for example), this mutant mouse strain may be useful in studies of iron homeostasis.

Development

A targeting vector containing a floxed NEO-CD(cytidine deaminase) cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 6 of the targeted gene, and another loxP site was inserted downstream of exon 7. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected in C57BL/6 blastocysts. The resulting chimeric animals were crossed to 129S6/SvEvTac mice, and then crossed to mice carrying the Tg(EIIa-cre)C5379Lmgd transgene on the 129S6/SvEvTac to remove the floxed selection cassette. Mice that still retained the loxP-flanked exons 6 and 7 were then backcrossed to 129S6/SvEvTac for more than ten generations.

Allele

Symbol: Slc40a1^tm2Nca
Name: targeted mutation 2, Nancy C Andrews
MGI accession ID: MGI:3771548
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Slc40a1
Marker name: solute carrier family 40 (iron-regulated transporter), member 1
Chromosome: 1
Strain of origin: 129S4/SvJae
Molecular note: A floxed neo-cassette was inserted in intron 5 and a third loxP site was inserted in intron 7 via homologous recombination. The floxed neo cassette was removed by in vivo cre-mediated recombination leaving exons 6 and 7 flanked by loxP sites.