These Dmt1fl mice possess loxP sites flanking exons 6-8 of the solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (Slc11a2) gene and have applications in studies related to anemia, iron homeostasis and micronutrient and drug absorption in the intestine.
Nancy C. Andrews, Duke University School of Medicine
The divalent metal transporter DMT1, encoded by Slc11a2, is essential for iron and micronutrient uptake in the gut and other tissues. These mice possess loxP sites on either side of exons 6 through 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 through 8 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in hippocampal neurons, this mutant mouse strain may be useful in studies of the role of iron in neurodevelopment, neurobiology and neurodegenerative diseases.
A targeting vector containing a floxed NEO-CD(cytidine deaminase) cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 8 of the targeted gene, and another loxP site was inserted upstream of exon 6. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. ES cells that had successfully undergone Cre-mediated recombination and no longer retained the cassette but did retain the loxP-flanked exons 6-8 were injected in C57BL/6 blastocysts. The resulting chimeric animals were crossed to 129S6/SvEvTac mice, and then backcrossed to 129S6/SvEvTac for more than 10 generations.
|Allele Name||targeted mutation 2, Nancy C Andrews|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Slc11a2, solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A single loxP site was inserted into intron 5 and a loxP flanked cytidine deaminase (CD)- neomycin cassette was inserted into intron 8. Transient cre expression removed the CD-neo cassette.|
|Mutations Made By|| |
Nancy Andrews, Duke University School of Medicine
When maintaining a live colony, these mice can be bred as homozygotes.
When using the 129S-Slc11a2tm2Nca/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017789 in your Materials and Methods section.