These p53FRT mice have exons 2-6 of the pro-apoptotic transformation related protein 53 gene (Trp53) flanked by Frt-sites, allowing for disruption of gene expression.
David Kirsch, Duke University Medical Center
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Trp53 | transformation related protein 53 |
In these mice, frt sites flank exons 2-6 of the transformation related protein 53 (Trp53) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. P53, encoded by the Trp53 gene, is a pro-apoptotic tumor suppressor protein. Exons 2-6 encode the DNA binding domain which is required for p53-dependent tumor suppression. Flp-mediated recombination in these mice results in the deletion of exons 2-6 in the FLP-expressing tissues of offspring. For example, when bred to B6;129-Krastm5Tyj/J (Stock No. 008653) mice, administration of a Flp-expressing adenovirus results in the development of lung tumors after inhalation of Adeno-FlpO and sarcomas after injection of Adeno-FlpO.
A targeting vector was designed to insert a Frt site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette followed by a second frt site downstream of exon 6 of the transformation related protein 53 (Trp53) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice. Offspring were bred with B6.129S4-Meox2tm1(cre)Sor/J transgenic mice to delete the neo cassette. These mice were maintained on a mixed B6;129S background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1.1, David G Kirsch |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | p53FRT |
Gene Symbol and Name | Trp53, transformation related protein 53 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 11 |
Molecular Note | A targeting vector was designed to insert a FRT site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette followed by a second FRT site downstream of exon 6. Cre-mediated recombination removed the neo cassette. |
Mutations Made By | David Kirsch, Duke University Medical Center |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129-Trp53tm1.1Dgk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017767 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Trp53<tm1.1Dgk> |
Frozen Mouse Embryo | B6;129-Trp53<tm1.1Dgk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Trp53<tm1.1Dgk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Trp53<tm1.1Dgk>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Trp53<tm1.1Dgk>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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