B6;129-Trp53^tm1.1Dgk/J

Stock number: 017767
Research areas: Cancer Research, Cell Biology Research, Research Tools

Description

These p53^FRT mice have exons 2-6 of the pro-apoptotic transformation related protein 53 gene (Trp53) flanked by Frt-sites, allowing for disruption of gene expression.

Detailed description

In these mice, frt sites flank exons 2-6 of the transformation related protein 53 (Trp53) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. P53, encoded by the Trp53 gene, is a pro-apoptotic tumor suppressor protein. Exons 2-6 encode the DNA binding domain which is required for p53-dependent tumor suppression. Flp-mediated recombination in these mice results in the deletion of exons 2-6 in the FLP-expressing tissues of offspring. For example, when bred to B6;129-Kras^tm5Tyj/J (Stock No. 008653) mice, administration of a Flp-expressing adenovirus results in the development of lung tumors after inhalation of Adeno-FlpO and sarcomas after injection of Adeno-FlpO.

Development

A targeting vector was designed to insert a Frt site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette followed by a second frt site downstream of exon 6 of the transformation related protein 53 (Trp53) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice. Offspring were bred with B6.129S4-Meox2^tm1(cre)Sor/J transgenic mice to delete the neo cassette. These mice were maintained on a mixed B6;129S background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Trp53^tm1.1Dgk
Name: targeted mutation 1.1, David G Kirsch
MGI accession ID: MGI:5306612
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Trp53
Marker name: transformation related protein 53
Chromosome: 11
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was designed to insert a FRT site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette followed by a second FRT site downstream of exon 6. Cre-mediated recombination removed the neo cassette.