B6Ei.LT-Y(IsXPAR;Y)Ei Tyrp1^B-lt/EiJ

Stock number: 017764
Product name: Y* brown

Description

Male mice of this strain carry an abnormal Y chromosome that can be used as a cytological marker and may be useful in studies of spermatogenesis.

Detailed description

This strain contains an abnormal LT/Sv-derived Y Chromosome comprising a spontaneous rearrangement (Eicher et al. 1991), formally designated Y(IsXPAR;Y)Ei and informally called Y*. Burgoyne et. al, (1998), updated the original description of the cytogenetic changes in this abnormal Y Chromosome, as an end-to-end fusion of two pseudoautosomal regions (PAR). Males with this abnormal Y Chromosome (XY*) are fully fertile and can be used to transmit this chromosomal aberration. Duplication of the PAR in Y* males permits generation of X and Y reciprocal translocation products during meiosis (see figure 1 in Eicher et.al 1991). Sperm produced by XY* males contain either a normal X Chromosome, the intact, complete Y^*, a large marker sex chromosome comprising an X Chromosome and most of the Y (Y^*), or a tiny cytogenetic marker sex chromosome (Y^X). Among offspring are the following genotypes and corresponding phenotypes:
XY^*, which are fully fertile males
XX, normal females
XX^Y*, which are sterile males in which gonad development is normal but spermatogenesis fails resulting in smaller testes after puberty. These males can be classified by manually palpating for testes size after 6 weeks of age. The testes are significantly smaller compared with any normal control male of the same age. Also these mice have a very large marker Chromosome (Fig 1, Eicher et al. 1991) easily seen after simple Giemsa staining (G-banding is not necessary. Alternatively these mice have nearly 2 complete X Chromosomes so these mice can be differentiated from normal XY<*> males by an Xist expression assay (Werler et al. 2011).
XY^*X females, which are fertile but litter size and number of litters produced will be less than expected for C57BL/6. These mice have a tiny cytogenetic marker (Fig 1, Eicher et al. 1991) easily seen after Giemsa staining of mitotic metaphase preparations.

Development

Y(IsXPAR;Y)Ei, also called Y*, arose during the backcrossing of Pgk1^a from wild-derived mice of Danish origin onto the LT/Sv inbred background (Eicher et al., 1991). Sterile males with small testes and a very large X chromosome by cytology were identified in the ninth backcross generation. Later this mutation was backcrossed along with Tyrp1^B-lt from the strain LT/SvEi-Y* onto C57BL/6JEi by consecutive crosses of a male carrying an abnormal LT Y chromosome, XY^*, to a C57BL/6JEi female. In 1994 embryos for this strain were cryopreserved from C57BL/6JEi females bred to carrier males at backcross generation N21, still segregating for Tyrp1^B-lt. For a cryopreserved sperm bankstock with Tyrp1^B-lt bred out please see stock No. 002021.

Allele

Symbol: Tyrp1^B-lt
Name: light
MGI accession ID: MGI:1855962
Generation method: Spontaneous
Marker symbol: Tyrp1
Marker name: tyrosinase-related protein 1
Chromosome: 4
Strain of origin: C58
Molecular note: A C-to-T transition at coding nucleotide 112 is predicted to result in an arginine to cysteine substitition at codon 38 (p.R38C).

Allele

Symbol: Y(IsXPAR;Y)Ei
Name: Chr Y, insertion of X pseudoautosomal region to Y pseudoautosomal region, Eicher
MGI accession ID: MGI:5300493
Generation method: Spontaneous
Marker symbol: Y(IsXPAR;Y)Ei
Marker name: Chr Y, insertion of X PAR region to Y PAR region, Eva Eicher
Chromosome: Y
Strain of origin: LT/Sv
Molecular note: The Y* PAR chromosome has resulted from an end to end fusion of an X and a Y PAR. Furthermore, it was shown that in conjuction with this PAR-PAR fusion, there has been a deletion of both copies of the distally located pseudoautosomal gene Steroid sulfatase (Sts). The arrangement of the two PAR regions permits reciprocal translocation of X and Y chromatin at every meiotic event in the male resulting in half of the sperm produced bearing one of two abnormal sex chromosome.