A NEO cassette replaces exon 8 of the Serpinb3a gene in these mice resulting in a reduced sensitivity to certain allergens.
Gary A. Silverman, University of Pittsburgh
The mouse Serpinb3a gene is similar to the human SERPINB4 and SERPINB3 genes, which have been associated with allergic reactions and asthma. Mouse Serpinb3a inhibits both serine and cysteine proteinases. These mice carry a null targeted mutation of Serpinb3a; exon 8 is disrupted. No gene product (mRNA) is detected by RT-PCR analysis of homozygotes. Serpinb3a-deficient mice are less sensitive to house dust mite-induced airway hyper-responsiveness, with reduced goblet cell hyperplasia and mucus production when compared to wildtype controls. IL13-induced airway hyper-responsiveness is reduced in homozygotes, with a reduced number of inflammatory cells in the bronchoalveolar lavage fluid. Mice that are homozygous for the targeted mutation are viable and fertile.
A targeting vector containing a NEO cassette was used to disrupt exon 8. The construct was electroporated into unspecified 129 (129/SV) derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to BALB/cAnNCrl
mice, and then backcrossed to BALB/cAnNCrl
for at least 5 generations.
Upon arrival at The Jackson Laboratory, the mice were crossed to BALB/cByJ (Stock No. 001026) at least once to establish the colony.
|Allele Name||targeted mutation 1, Gary A Silverman|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Serpinb3a, serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 3A|
|Strain of Origin||129|
|Molecular Note||A targeting vector containing a NEO cassette replaced most of exon 8. RT-PCR confirmed the absence of transcript expression.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the C.129-Serpinb3atm1Gsil/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017740 in your Materials and Methods section.