C.129-Serpinb3a^tm1Gsil/J

Stock number: 017740
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

A NEO cassette replaces exon 8 of the Serpinb3a gene in these mice resulting in a reduced sensitivity to certain allergens.

Detailed description

The mouse Serpinb3a gene is similar to the human SERPINB4 and SERPINB3 genes, which have been associated with allergic reactions and asthma. Mouse Serpinb3a inhibits both serine and cysteine proteinases. These mice carry a null targeted mutation of Serpinb3a; exon 8 is disrupted. No gene product (mRNA) is detected by RT-PCR analysis of homozygotes. Serpinb3a-deficient mice are less sensitive to house dust mite-induced airway hyper-responsiveness, with reduced goblet cell hyperplasia and mucus production when compared to wildtype controls. IL13-induced airway hyper-responsiveness is reduced in homozygotes, with a reduced number of inflammatory cells in the bronchoalveolar lavage fluid. Mice that are homozygous for the targeted mutation are viable and fertile.

Development

A targeting vector containing a NEO cassette was used to disrupt exon 8. The construct was electroporated into unspecified 129 (129/SV) derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to BALB/cAnNCrl mice, and then backcrossed to BALB/cAnNCrl for at least 5 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to BALB/cByJ (Stock No. 001026) at least once to establish the colony.

Allele

Symbol: Serpinb3a^tm1Gsil
Name: targeted mutation 1, Gary A Silverman
MGI accession ID: MGI:5447741
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Serpinb3a
Marker name: serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 3A
Chromosome: 1
Strain of origin: 129
Molecular note: A targeting vector containing a NEO cassette replaced most of exon 8. RT-PCR confirmed the absence of transcript expression.