Hemizygotes express tetracycline-controlled transactivator protein (tTA) in bone marrow hematopoietic stem cells, in common myeloid progenitors, and in lung tissue. They can be used to generate bitrangenic animals to study inducible gene expression in blood stem and progenitor cells
Daniel G. Tenen, Beth Israel Deaconess Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Transactivator) |
Hemizygotes are viable, fertile, normal in size, and do not display any behavioral abnormalities. Hemizygotes express tetracycline-controlled transactivator protein (tTA) in bone marrow hematopoietic stem cells (HSC) and in common myeloid progenitors (CMP)). tTA activity also is detected in lung. Little to no tTA activity is detected in thymus, lymph node, intestine or spleen. When bred to other transgenic mice carrying a gene of interest coupled to a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring can be induced by withdrawal of tetracycline or doxycycline. This strain represents an effective tool for generating bitrangenic animals to study inducible gene expression in blood stem and progenitor cells.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct consisting of the SV40 minimal promoter, rabbit beta-globin intron 2, and the 3' enhancer sequence from the murine T-cell acute lymphocytic leukemia 1 gene (Tal1, formerly called homolog of human stem cell leukemia gene or SCL) was designed to direct expression of the tetracycline-regulated transactivator, tTA. The construct was injected into the pronucleus of "BDF" (C57BL/6 x DBA/2 F1 hybrid) eggs, which subsequently were implanted into pseudopregnant foster mothers. Transgenic mice were bred to generate founder line 19. In 2004, mice were bred to a "tetO-cre" transgenic mouse on an unidentified background. Subsequently, mice were selected for the Tal1-tTA transgene (and against tetO-cre) while backcrossing to FVB/N for approximately 7-9 generations. The mice were then backcrossed to C57BL/6 for 11 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
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Site of Expression | Expresses tTA in bone marrow-derived hematopoietic stem cells (HSC) and common myeloid progenitors (CMP). tTA activity also is detected in lung. |
Allele Name | transgene insertion 19, Daniel G Tenen |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | SCLtTA; Scl-tTA; SCLtTA stg |
Gene Symbol and Name | Tg(Tal1-tTA)19Dgt, transgene insertion 19, Daniel G Tenen |
Gene Synonym(s) | |
Promoter | SV40, E mu enhancer, SV40 |
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
Site of Expression | Expresses tTA in bone marrow-derived hematopoietic stem cells (HSC) and common myeloid progenitors (CMP). tTA activity also is detected in lung. |
Strain of Origin | (C57BL/6 x DBA/2)F1 |
Chromosome | UN |
Molecular Note | The transgene consists of the SV40 minimal promoter, rabbit beta-globin intron 2, and the 3' enhancer sequence from the mouse T-cell acute lymphocytic leukemia gene driving expression of the tetracycline-regulated transactivator. |
Mutations Made By | Daniel Tenen, Beth Israel Deaconess Medical Center |
When maintaining a live colony, transgenic mice can be bred to wildtype siblings.
When using the SCLtTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #017722 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Tal1-tTA)19Dgt |
Frozen Mouse Embryo | B6.Cg-Tg(Tal1-tTA)19Dgt/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Tal1-tTA)19Dgt/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Tal1-tTA)19Dgt/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Tal1-tTA)19Dgt/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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