These p110αflox (p110alphaflox) mice possess loxP sites flanking exon 1 of the phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) gene. This strain may be useful for studying insulin signaling, hepatic glucose and lipid metabolism, and oncogenic transformation.
Jean Zhao, Dana Farber Cancer Institute
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Pik3ca | phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha |
These p110αflox (p110alphaflox) mice possess loxP sites flanking exon 1 of the phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) gene. P110α is an isoform of the p110 catalytic subunit of PI3K, which is a critical enzyme in the insulin signaling pathway. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in cre-expressing tissues. For example, when crossed to a strain expressing Cre recombinase in the liver, this mutant mouse strain displays reduced insulin sensitivity, impaired glucose tolerance, and increased gluconeogenesis, hypolipidemia, and hyperleptinemia. They also exhibit a 34% reduction in serum free fatty acids, a 28% reduction in total serum cholesterol, and a 44% reduction in serum triglycerides, as well as decreased lipogenic gene expression and hepatic triglyceride content. Their level of serum leptin and leptin receptor expression are increased 8 fold compared to control mice. This strain may be useful for studying insulin signaling, hepatic glucose and lipid metabolism, and oncogenic transformation.
A targeting vector was designed to insert a loxP site upstream of exon 1, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site were placed downstream of exon 1 of the phosphatidylinositol 3-kinase, catalytic, alpha polypeptide (Pik3ca) gene. The construct was electroporated into 129 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pCAGGS-FLPe expression plasmid to delete the neo cassette.
Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to C57BL/6NTac females. These p110αflox mice were backcrossed to C57BL/6NTac for at least 10 generations. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Jean J Zhao |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | p110alpha flox; p110alphaflox |
Gene Symbol and Name | Pik3ca, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 3 |
Molecular Note | Exon 1 was flanked by lox P sites, with an FRT-flanked selection cassette between exon 1 and the downstream loxP site. The selection cassette was subsequently removed from the locus via transient transfection with a FLP recombinase plasmid. |
Mutations Made By | Jean Zhao, Dana Farber Cancer Institute |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6N.129-Pik3catm1Jjz/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017704 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Pik3ca<tm1Jjz> |
Frozen Mouse Embryo | B6N.129-Pik3ca<tm1Jjz>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129-Pik3ca<tm1Jjz>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129-Pik3ca<tm1Jjz>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.129-Pik3ca<tm1Jjz>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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