In this this knockout strain exons 3-5 of the CD83 antigen (Cd83) gene are disrupted, abolishing gene function. These mice have reduced numbers of CD4+ T cells, and may be useful for studying lymphocyte development and immune regulation.
Dr. Thomas F. Tedder, Duke University Medical Center
A neo cassette replaces part of exons 3-5 of the CD83 antigen (Cd83) gene abolishing gene function in this strain. CD83 is a cell surface protein expressed by dendritic cells (DCs) and thymic epithelial cells. It plays a role in the maturation of CD4+ T cells. Homozygotes are viable, fertile and normal in size. These mice exhibit a 68% reduction in CD4 single-positive thymocytes, a 75-90% reduction in peripheral CD4+ T cells, and an absence of naive CD4+ T cells. They also show a 25-50% reduction of cell-surface class II antigen expression on splenic B cells and DCs, thymic epithelial cells and peritoneal macrophages. Survival of B cells and CD4+ T cells in these Cd83-/- mice is decreased. These mice may be useful for studying lymphocyte development and immune regulation.
A targeting vector was designed to replace parts exons 3-5 of the CD83 antigen (Cd83) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S4/SvJaeSor-derived AK-7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to C57BL/6J mice. These mice were backcrossed at least 9 generations to C57BL/6J background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Thomas F Tedder|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Cd83, CD83 antigen|
|Strain of Origin||129S4/SvJaeSor|
|Molecular Note||A sequence fragment extending from within exon 3 through the coding region of exon 5 was replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded 25 residues of the immunoglobulin-like domain and the transmembrane and cytoplasmic domains. Transcript was undetected in homozygous mutant mice by RT-PCR analysis of LPS-stimulated bone marrow-derived dendritic cells.|
|Mutations Made By|| |
Dr. Thomas Tedder, Duke University Medical Center
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129S4-Cd83tm1Tft/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017703 in your Materials and Methods section.