For the Pink1^tm1Aub targeted mutation strain, a targeting construct containing a nucleotide substitution from a G to an A at nucleotide 8343 in exon 5, a loxP-flanked NEO cassette, was electroporated into a unspecified 129/SvEv-derived embryonic stem (ES) cell line. Correctly targeted ES cells were injected into blastocysts. The colony was maintained on a 129/SvEv background.

For the Tg(Prnp-SNCA*A53T)AAub transgenic strain, a transgenic construct containing the entire translated portion of the mutant A53T human alpha-synuclein (SNCA) from position 22 to 515, driven by an ~3.5 kb fragment of the mouse prion protein promoter (Prnp), and polyadenylation signal sequence, was injected into fertilized FVB/N mouse eggs. Founder line A was subsequently established.

The strains were then intercrossed to generate double mutant mice that were heterozygous for the Pink1^tm1Aub allele and Tg(Prnp-SNCA*A53T)AAub transgene. The double heterozygotes were then crossed to generate mice homozygous for both mutations. The mice were maintained on a mixed 129;FVB background. Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.

Approximate Controls

• 001800 FVB/NJ

Additional Information

• Considerations for Choosing Controls

• Cabin DE; Gispert-Sanchez S; Murphy D; Auburger G; Myers RR; Nussbaum RL. 2005. Exacerbated synucleinopathy in mice expressing A53T SNCA on a Snca null background. Neurobiol Aging 26(1):25-35PubMed: 15585343MGI: J:124976
• Gispert S; Brehm N; Weil J; Seidel K; Rub U; Kern B; Walter M; Roeper J; Auburger G. 2015. Potentiation of neurotoxicity in double-mutant mice with Pink1 ablation and A53T-SNCA overexpression. Hum Mol Genet 24(4):1061-76PubMed: 25296918MGI: J:214065
• Gispert S; Del Turco D; Garrett L; Chen A; Bernard DJ; Hamm-Clement J; Korf HW; Deller T; Braak H; Auburger G; Nussbaum RL. 2003. Transgenic mice expressing mutant A53T human alpha-synuclein show neuronal dysfunction in the absence of aggregate formation. Mol Cell Neurosci 24(2):419-29PubMed: 14572463MGI: J:86222
• Gispert S; Ricciardi F; Kurz A; Azizov M; Hoepken HH; Becker D; Voos W; Leuner K; Muller WE; Kudin AP; Kunz WS; Zimmermann A; Roeper J; Wenzel D; Jendrach M; Garcia-Arencibia M; Fernandez-Ruiz J; Huber L; Rohrer H; Barrera M; Reichert AS; Rub U; Chen A; Nussbaum RL; Auburger G. 2009. Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration. PLoS One 4(6):e5777PubMed: 19492057MGI: J:150206
• Kurz A; Double KL; Lastres-Becker I; Tozzi A; Tantucci M; Bockhart V; Bonin M; Garcia-Arencibia M; Nuber S; Schlaudraff F; Liss B; Fernandez-Ruiz J; Gerlach M; Wullner U; Luddens H; Calabresi P; Auburger G; Gispert S. 2010. A53T-alpha-synuclein overexpression impairs dopamine signaling and striatal synaptic plasticity in old mice. PLoS One 5(7):e11464PubMed: 20628651MGI: J:163115