This knockout strain lacks the entire coding region of the Ccl7 gene. These mice may be useful for studying the role of MCP-3 during monocyte recruitment and homeostasis.
Israel F. Charo, Gladstone Inst of Cardiovascular Disease, UCSF
In this strain a neo cassette replaces the entire coding region of the chemokine (C-C motif) ligand 7 (Ccl7) gene, abolishing gene function. CCL7, also known as MCP-3 (monocyte chemoattractant protein 3), directs the migration of monocytes to sites of inflammation through activation of the monocyte surface receptor CCR2 (chemokine (C-C motif) receptor 2). Homozygous CCL7 deficient mice are viable, fertile, and normal in size. These mice exhibit a 65% reduction of the 7/4 bri Ly-6G monocytes. These mice may be useful for studying the role of MCP-3 in monocyte recruitment and homeostasis.
A targeting vector was designed to replace the coding region of the chemokine (C-C motif) ligand 7 (Ccl7) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S4/SvJae-derived RF8 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6J mice. These mice were backcrossed to C57BL/6J background for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Israel F Charo|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Ccl7, chemokine (C-C motif) ligand 7|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A targeting vector containing a neomycin resistance cassette was inserted to replace the coding sequence.|
|Mutations Made By|| |
Israel Charo, Gladstone Inst of Cardiovascular Disease, UCSF
When maintaining a live colony, homozygous mice may be bred together.
When using the Mcp-3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #017638 in your Materials and Methods section.