In this strain a NEO cassette replaces the entire Nrarp, Notch-regulated ankyrin repeat protein, gene coding sequences. Homozygotes on the 129 background exhibit anterior-posterior somite patterning defects and abnormal axial skeleton morphology.
Thomas Gridley, Maine Medical Center Research Institute
Nrarp, Notch-regulated ankyrin repeat protein, is a Notch target gene and part of a negative feedback loop for Notch pathway signaling. Mice that are homozygous for the targeted mutation viable, fertile, and display axial skeleton defects and anterior-posterior somite patterning defects. Homozygotes exhibit thoracic, lumbar, sacral and caudal vertebral abnormalities, including proximal rib fusions, and vertebral body and pedicle fusions. PCR analysis confirms the allele is a null.
A targeting vector containing a loxP site flanked NEO cassette was used to disrupt all coding sequence. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S1/SvImJ mice. Upon transfer to The Jackson Laboratory, the mice were crossed to 129S1/SvImJ (Stock No. 002448) at least once to establish the colony.
|Allele Name||targeted mutation 1, Tom Gridley|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Nrarp, Notch-regulated ankyrin repeat protein|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A floxed neo cassette replaced the coding sequence. PCR confirmed the absence of the coding sequence.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Nrarp2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #017631 in your Materials and Methods section.