In this strain a NEO cassette replaces the entire Nrarp, Notch-regulated ankyrin repeat protein, gene coding sequences. Homozygotes on the 129 background exhibit anterior-posterior somite patterning defects and abnormal axial skeleton morphology.
Thomas Gridley, Maine Medical Center Research Institute
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Nrarp | Notch-regulated ankyrin repeat protein |
Nrarp, Notch-regulated ankyrin repeat protein, is a Notch target gene and part of a negative feedback loop for Notch pathway signaling. Mice that are homozygous for the targeted mutation viable, fertile, and display axial skeleton defects and anterior-posterior somite patterning defects. Homozygotes exhibit thoracic, lumbar, sacral and caudal vertebral abnormalities, including proximal rib fusions, and vertebral body and pedicle fusions. PCR analysis confirms the allele is a null.
A targeting vector containing a loxP site flanked NEO cassette was used to disrupt all coding sequence. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S1/SvImJ mice. Upon transfer to The Jackson Laboratory, the mice were crossed to 129S1/SvImJ (Stock No. 002448) at least once to establish the colony.
Allele Name | targeted mutation 1, Tom Gridley |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Nrarp, Notch-regulated ankyrin repeat protein |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 2 |
Molecular Note | A floxed neo cassette replaced the coding sequence. PCR confirmed the absence of the coding sequence. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Nrarp2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #017631 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Nrarp<tm1Grid> |
Frozen Mouse Embryo | 129-Nrarp<tm1Grid>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129-Nrarp<tm1Grid>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | 129-Nrarp<tm1Grid>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | 129-Nrarp<tm1Grid>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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