These Ccl11 knockout mice exhibit a reduced total eosinophil count in peripheral blood and may be useful for studying eosinophilia and inflammatory disorders.
Marc Rothenberg, Cincinnati Children's Hospital
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ccl11 | chemokine (C-C motif) ligand 11 |
Mice homozygous for the Ccl11tm1Mer allele contain a neomycin resistance cassette that replaces exon 2 and part of exon 3, abolishing gene expression. CCL11 (or eotaxin) is an eosinphil specific C-C chemokine involved in eosinophil recruitment. Mice deficient in eotaxin exhibit a reduced total eosinophil count in peripheral blood. Following antigen challenge, eosinophils are significantly reduced in the early, but not late, phase of the inflammatory response. Homozygotes are viable, fertile, normal in size, and do not display any gross physical abnormalities. These mice may be useful for studying eosinophilia in peripheral blood and inflammatory disorders.
A targeting vector containing a PGKneo cassette was used to disrupt all of exon 2, which encodes the mature protein, and most of exon 3. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to outbred mice and maintained on a mixed background.
Allele Name | targeted mutation 1, Marc E Rothenberg |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Eot-; eotaxin-; eotaxin-1 |
Gene Symbol and Name | Ccl11, chemokine (C-C motif) ligand 11 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 11 |
Molecular Note | All of exon 2 and the majority of exon 3 were replaced by a neomycin selection cassette. In addition to containing the 3' UTR, the deleted region encoded most of the mature protein. Transcript was undetected in homozygous mutant mice via Northern blot analysis of skin and lung RNA (the latter of which was isolated from mice treated with and subsequently challenged with OVA). |
Mutations Made By | Marc Rothenberg, Cincinnati Children's Hospital |
While maintaining a live colony, these mice are bred as homozygotes.
When using the C.129S6(Cg)-Ccl11tm1Mer/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017618 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ccl11<tm1Mer> |
Frozen Mouse Embryo | C.129S6(Cg)-Ccl11<tm1Mer>/J | $2595.00 |
Frozen Mouse Embryo | C.129S6(Cg)-Ccl11<tm1Mer>/J | $2595.00 |
Frozen Mouse Embryo | C.129S6(Cg)-Ccl11<tm1Mer>/J | $3373.50 |
Frozen Mouse Embryo | C.129S6(Cg)-Ccl11<tm1Mer>/J | $3373.50 |
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