B6(Cg)-Tyr^c-2J Tg(UBC-mCherry)1Phbs/J

Stock number: 017614
Strain synonyms: B6(Cg)-Tyrc-2J Ing3^{Tg(UBC-mCherry)1Phbs}/J
Research areas: Dermatology Research, Mouse/Human Gene Homologs, Research Tools, Sensorineural Research

Description

These B6;albino;mCherry transgenic mice express monomeric red fluorescent protein (mCherry) under the direction of the human ubiqutin C promoter. Expression is observed in almost all tissues examined and may be useful as a bright and photostable means for visualizing morphogenesis and tissue rearrangements alone or in combination with mice expressing eGFP.

Detailed description

These transgenic mice express monomeric red fluorescent protein (mCherry) under the direction of the human ubiqutin C promoter. Mice hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express mCherry in almost all tissues examined including pre- and post-implantation stage embryos, neonatal pups and adult mice. mCherry is strongly expressed in brain, testis, bladder, prostate and epipdymis and at lower levels in other tissues. No expression is detected in erythrocytes or thrombocytes. These mice may be useful as a bright and photostable means for visualizing morphogenesis and tissue rearrangements alone or in combination with mice expressing eGFP.

Development

Lentiviral particles containing the monomeric red fluorescent protein, mCherry under the control of the human ubiqutin C promoter were produced and co-cultured with denuded two cell C57BL/6 embryos. Founder mice were bred to B6(Cg)-Tyr^c-2J/J (B6-albino) mice for at least ten generations. Mice were screened for mCherry expression in multiple tissues and developmental stages. Upon arrival at The Jackson Laboratory Repository, mice were bred with B6(Cg)-Tyr^c-2J/J mice (Stock No. 000058) for at least one generation to establish the colony.

Allele

Symbol: Tyr^c-2J
Name: albino 2 Jackson
MGI accession ID: MGI:1855985
Generation method: Spontaneous
Attributes: Null/Knockout
Marker symbol: Tyr
Marker name: tyrosinase
Marker synonyms: ATN; CMM8; Oca1; OCA1A; OCAIA; SHEP3; skc35
Chromosome: 7
Strain of origin: B6.Cg-Tyrp1^b Hps1^ep
Site of expression: Expressed in melanosomes
Molecular note: This mutation has a G-to-T base change at coding nucleotide 230 resulting in an amino acid change from arginine to leucine at residue 77 (p.R77L) which lies in the highly conserved DDRE sequence. Nucleotide 230 is at the alternative 5' splice donor site for exon 1 and this allele does not produce the 1a or 1b subset of tyrosinase transcripts but does produce a significant increase in 1c and 1d transcripts. Western blots of homozygous mutant skin extracts demostrate the nearly complete absence of the broad 76-84 kDa band of glycosylated wild-type tyrosinase. No tyrosinase activity was found in hairbulb extracts from homozygous mice.

Allele

Symbol: Tg(UBC-mCherry)1Phbs
Name: transgene lentiviral insertion 1, Poul Sorensen
MGI accession ID: MGI:5296812
Generation method: Transgenic
Attributes: Reporter
Synonyms: mCherry-tg; Ing3^{Tg(UBC-mCherry)1Phbs}
Marker symbol: Tg(UBC-mCherry)1Phbs
Marker name: transgene lentiviral insertion 1, Poul Sorensen
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: RFP,Red Fluorescent Protein,coral
Site of expression: mCherry is expressed in almost all tissues examined including pre- and post-implantation stage embryos, neonatal pups, and adult mice.
Molecular note: The transgene contains a lentiviral vector containing the monomeric red fluorescent protein, mCherry, under the control of the human ubiqutin C promoter. Line 1 was generated. The integration site of the UbC-mCherry cassette was detected at 6qA3.1 within intron 5, disrupting the Ing3 locus by uncharacterized mechanisms.