These B6;albino;mCherry transgenic mice express monomeric red fluorescent protein (mCherry) under the direction of the human ubiqutin C promoter. Expression is observed in almost all tissues examined and may be useful as a bright and photostable means for visualizing morphogenesis and tissue rearrangements alone or in combination with mice expressing eGFP.
Poul HB Sorensen, The University of British Columbia
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Spontaneous (Null/Knockout) | Tyr | tyrosinase |
Allele Type |
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Transgenic (Reporter) |
These transgenic mice express monomeric red fluorescent protein (mCherry) under the direction of the human ubiqutin C promoter. Mice hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express mCherry in almost all tissues examined including pre- and post-implantation stage embryos, neonatal pups and adult mice. mCherry is strongly expressed in brain, testis, bladder, prostate and epipdymis and at lower levels in other tissues. No expression is detected in erythrocytes or thrombocytes. These mice may be useful as a bright and photostable means for visualizing morphogenesis and tissue rearrangements alone or in combination with mice expressing eGFP.
Lentiviral particles containing the monomeric red fluorescent protein, mCherry under the control of the human ubiqutin C promoter were produced and co-cultured with denuded two cell C57BL/6 embryos. Founder mice were bred to B6(Cg)-Tyrc-2J/J (B6-albino) mice for at least ten generations. Mice were screened for mCherry expression in multiple tissues and developmental stages. Upon arrival at The Jackson Laboratory Repository, mice were bred with B6(Cg)-Tyrc-2J/J mice (Stock No. 000058) for at least one generation to establish the colony.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression | mCherry is expressed in almost all tissues examined including pre- and post-implantation stage embryos, neonatal pups, and adult mice. |
Allele Name | albino 2 Jackson |
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Allele Type | Spontaneous (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Tyr, tyrosinase |
Gene Synonym(s) | |
Site of Expression | Expressed in melanosomes |
Strain of Origin | B6.Cg-Tyrp1b Hps1ep |
Chromosome | 7 |
Molecular Note | This mutation has a G-to-T base change at coding nucleotide 230 resulting in an amino acid change from arginine to leucine at residue 77 (p.R77L) which lies in the highly conserved DDRE sequence. Nucleotide 230 is at the alternative 5' splice donor site for exon 1 and this allele does not produce the 1a or 1b subset of tyrosinase transcripts but does produce a significant increase in 1c and 1d transcripts. Western blots of homozygous mutant skin extracts demostrate the nearly complete absence of the broad 76-84 kDa band of glycosylated wild-type tyrosinase. No tyrosinase activity was found in hairbulb extracts from homozygous mice. |
Allele Name | transgene lentiviral insertion 1, Poul Sorensen |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | mCherry-tg |
Gene Symbol and Name | Tg(UBC-mCherry)1Phbs, transgene lentiviral insertion 1, Poul Sorensen |
Gene Synonym(s) | |
Promoter | UBC, ubiquitin C, human |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | mCherry is expressed in almost all tissues examined including pre- and post-implantation stage embryos, neonatal pups, and adult mice. |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | The transgene contains a lentiviral vector containing the monomeric red fluorescent protein, mCherry, under the control of the human ubiqutin C promoter. Line 1 was generated. |
Mutations Made By | Poul Sorensen, The University of British Columbia |
While maintaining a live colony, these mice are bred as hemizygotes. The donating investigator indicates that homozygotes have not been assessed.
When using the B6(Cg)-Tyrc-2J Tg(UBC-mCherry)1Phbs/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017614 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for tyr, hemzygous or non carrier for Tg(UBC-mCherry)1Phbs |
Frozen Mouse Embryo | B6(Cg)-Tyr<c-2J> Tg(UBC-mCherry)1Phbs/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Tyr<c-2J> Tg(UBC-mCherry)1Phbs/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Tyr<c-2J> Tg(UBC-mCherry)1Phbs/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Tyr<c-2J> Tg(UBC-mCherry)1Phbs/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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