Overview

These Luci-TRE-SMN transgenic mice harbor the "new" pBI-L-SMN(B) transgene designed to express both luciferase and a full-length human SMN gene under the control of a bi-directional tet-responsive promoter. These "new" Luci-TRE-SMN(B) mice allow high levels of both luciferase and full-length human SMN expression to be regulated by the addition/removal of dox, and offer a means to generate mutant mice for Tet-Off/Tet-On and/or fluorescent protein studies of Spinal Muscular Atrophy (SMA).

Donating Investigator

Arthur H.M. Burghes, The Ohio State University

Genetic overview

Genetic Background	Generation

Tg(tetO-SMN2,-luc)#bAhmb

Allele Type
Transgenic (Reporter, Inducible, Inserted expressed sequence, Humanized sequence)

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Research Applications

Neurobiology Research
Research Tools
Developmental Biology Research

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Mice hemizygous for the "new" pBI-L-SMN(B) transgene ("new" Luci-TRE-SMN(B) transgene mice) are viable and fertile, with no reported phenotypic abnormalities. These Luci-TRE-SMN transgenic mice express luciferase and a full-length (exons 1-8) human SMN gene under the control of a bi-directional tet-responsive promoter (TRE or tetO). When bred with another mouse expressing tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), simultaneous luciferase and SMN expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring. These Luci-TRE-SMN transgenic mice are useful to generate mutant mice for Tet-Off/Tet-On and/or fluorescent protein studies of Spinal Muscular Atrophy.

Development

The Luci-TRE-SMN transgene was created by Dr. Arthur HM Burghes (The Ohio State University). The Luci-TRE-SMN transgenic construct used here ["new" pBI-L-SMN(B)] was designed with a luciferase sequence on one side, and a full-length human SMN2 cDNA sequence (encoding exons 1-8) on the other side of the P_bi-1 bi-directional tetracycline-responsive promoter. The 1.37 kb human SMN2 cDNA sequence was derived via RT-PCR performed on total RNA isolated from SMA type 1-derived patient fibroblast cells (Coriell Institute GM 3813; shown to express both full-length and truncated SMN2 transcripts). Compared to the "old" pBI-L-SMN construct, the "new" pBI-L-SMN(B) construct used here has a 31 bp sequence (including a hemagglutinin epitope tag sequence) deleted between the P_bi-1 promoter and 5' end of SMN2 cDNA, as well as additional SMN2 exon 8 UTR sequence downstream of the SMN2 cDNA. The P_bi-1 promoter contains the Tet-responsive element (TRE; seven copies of the 42-bp tet operator sequence [tetO]) placed between two minimal CMV enhancer-less promoters. The "new" Luci-TRE-SMN(B) transgene was microinjected into the male pronucleus of FVB/N embryos. Founder mice were bred with Camk2a-tTA mice (FVB background; see Stock No. 003010) to determine which lines had high dox-inducible levels of luciferase and SMN in brain. The high-expressing "new" Luci-TRE-SMN(B) founder line was identified and used to create the Tg(SMN2)89; Smn1^+/-; Tg(SMNΔ7)4299; ROSA26^rtTA; "new" Luci-TRE-SMN(B) mice (on a mixed FVB/N and C57BL/6 genetic background) that was sent to The Jackson Laboratory Repository as Stock No. 017597). Upon arrival, some mice were bred with FVB/NJ inbred mice (Stock No. 001800) for several generations to remove the other transgenes/mutations. The resulting mice harboring only the "new" Luci-TRE-SMN(B) transgene were assigned Stock No. 017600.

Expression Data

Expressed Gene	SMN2, survival of motor neuron 2, centromeric, human
Expressed Gene	luc, luciferase, firefly
Site of Expression	When bred to mice expressing a tetracycline transactivator (tTA) or reverse transactivator (rtTA), doxycycline induction results in the expression of luciferase and SMN expression in transactivator expressing tissues of the offspring.

Control Suggestions

Noncarrier
001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Selected References

Le TT; McGovern VL; Alwine IE; Wang X; Massoni-Laporte A; Rich MM; Burghes AH. 2011. Temporal requirement for high SMN expression in SMA mice. Hum Mol Genet 20(18):3578-91PubMed: 21672919 MGI: J:174960

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Genetics

Tg(tetO-SMN2,-luc)#bAhmb

Allele Symbol: Tg(tetO-SMN2,-luc)#bAhmb

Allele Name	transgene insertion, Arthur H M Burghes
Allele Type	Transgenic (Reporter, Inducible, Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	Tg(tetO-SMN2,-luc)#bAhmb; transgene insertion, Arthur H M Burghes
Gene Symbol and Name	Tg(tetO-SMN2,-luc)#bAhmb, transgene insertion, Arthur H M Burghes
Gene Synonym(s)
Promoter	tetO, tet operator,
Expressed Gene	SMN2, survival of motor neuron 2, centromeric, human
Expressed Gene	luc, luciferase, firefly
Site of Expression	When bred to mice expressing a tetracycline transactivator (tTA) or reverse transactivator (rtTA), doxycycline induction results in the expression of luciferase and SMN expression in transactivator expressing tissues of the offspring.
Strain of Origin	FVB/N
Chromosome	UN
Molecular Note	The tetracycline response element with two minimal CMV promoters drives inducible expression of human SMN (exons 1 through 8) and a luciferase gene.
Mutations Made By	Arthur Burghes, The Ohio State University

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Tet Expression System
  - tTA/rtTA Responsive Strains
- Neurodegeneration
- Fluorescent protein expression in neural tissue
- Spinal Muscular Atrophy (SMA)
- Ataxia (Movement) Defects
Research Tools
- Neurobiology Research
  - Tetop Tet System
  - cell marker
- Tet Expression Systems
  - tTA/rtTA Responsive Strains
- Genetics Research
  - Tissue/Cell Markers
  - Tissue/Cell Markers: neurons
  - Tissue/Cell Markers: multiple
Developmental Biology Research
- Neurodevelopmental Defects

Mammalian Phenotype Terms by Genotype

References

Le TT; McGovern VL; Alwine IE; Wang X; Massoni-Laporte A; Rich MM; Burghes AH. 2011. Temporal requirement for high SMN expression in SMA mice. Hum Mol Genet 20(18):3578-91PubMed: 21672919 MGI: J:174960

Additional - Tg(tetO-SMN2,-luc)#bAhmb related

Technical Support

Contact Technical Support

Genotyping Protocols
- Separated MCA: Tg(tetO-SMN,-luc)#Ahmb
- High Resolution Melting: Tg(tetO-SMN,-luc)#Ahmb
- Separated PCR: Tg(tetO-SMN,-luc)#Ahmb
- MELT: Grm7^{Tg(SMN2)89Ahmb} Alternate1
- Genotyping resources and troubleshooting
Breeding Considerations

Some Tg(SMN2)89; Smn1^+/-; Tg(SMNΔ7)4299; ROSA26^rtTA; "new" Luci-TRE-SMN(B) mice (Stock No. 017597) were bred with FVB/NJ inbred mice (Stock No. 001800) for several generations, selecting for the "new" Luci-TRE-SMN(B) transgene and to remove the other transgenes/mutations. The resulting mice harboring only the "new" Luci-TRE-SMN(B) transgene were assigned Stock No. 017600. When maintaining the live "new" Luci-TRE-SMN(B) transgenic colony, transgenic mice may be bred with wildtype (noncarrier) mice from the colony or with FVB/NJ inbred mice (Stock No. 001800).
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Tg(tetO-SMN2,-luc)#bAhmb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017600 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

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Live Mouse

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Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Hemizygous or Non Carrier for Tg(tetO-SMN2,-luc)#bAhmb

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(tetO-SMN2,-luc)#bAhmb

Allele Symbol: Tg(tetO-SMN2,-luc)#bAhmb

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Tg(tetO-SMN2,-luc)#bAhmb related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability