JAX Mouse Strain Datasheet - 017600

STOCK Tg(tetO-SMN2,-luc)#bAhmb/J

Stock No:: 017600

Transgenic

Cryo Recovery

Overview

These Luci-TRE-SMN transgenic mice harbor the "new" pBI-L-SMN(B) transgene designed to express both luciferase and a full-length human SMN gene under the control of a bi-directional tet-responsive promoter. These "new" Luci-TRE-SMN(B) mice allow high levels of both luciferase and full-length human SMN expression to be regulated by the addition/removal of dox, and offer a means to generate mutant mice for Tet-Off/Tet-On and/or fluorescent protein studies of Spinal Muscular Atrophy (SMA).

Donating Investigator

Arthur H.M. Burghes, The Ohio State University

Genetic overview

Genetic Background	Generation

Tg(tetO-SMN2,-luc)#bAhmb

Allele Type
Transgenic (Humanized sequence, Inducible, Inserted expressed sequence, Reporter)

View Genetics

Research Applications

Developmental Biology Research
Neurobiology Research
Research Tools

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Mice hemizygous for the "new" pBI-L-SMN(B) transgene ("new" Luci-TRE-SMN(B) transgene mice) are viable and fertile, with no reported phenotypic abnormalities. These Luci-TRE-SMN transgenic mice express luciferase and a full-length (exons 1-8) human SMN gene under the control of a bi-directional tet-responsive promoter (TRE or tetO). When bred with another mouse expressing tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), simultaneous luciferase and SMN expression can be regulated with the tetracycline analog doxycycline (dox) in the resulting double mutant offspring. These Luci-TRE-SMN transgenic mice are useful to generate mutant mice for Tet-Off/Tet-On and/or fluorescent protein studies of Spinal Muscular Atrophy.

Development

The Luci-TRE-SMN transgene was created by Dr. Arthur HM Burghes (The Ohio State University). The Luci-TRE-SMN transgenic construct used here ["new" pBI-L-SMN(B)] was designed with a luciferase sequence on one side, and a full-length human SMN2 cDNA sequence (encoding exons 1-8) on the other side of the P_bi-1 bi-directional tetracycline-responsive promoter. The 1.37 kb human SMN2 cDNA sequence was derived via RT-PCR performed on total RNA isolated from SMA type 1-derived patient fibroblast cells (Coriell Institute GM 3813; shown to express both full-length and truncated SMN2 transcripts). Compared to the "old" pBI-L-SMN construct, the "new" pBI-L-SMN(B) construct used here has a 31 bp sequence (including a hemagglutinin epitope tag sequence) deleted between the P_bi-1 promoter and 5' end of SMN2 cDNA, as well as additional SMN2 exon 8 UTR sequence downstream of the SMN2 cDNA. The P_bi-1 promoter contains the Tet-responsive element (TRE; seven copies of the 42-bp tet operator sequence [tetO]) placed between two minimal CMV enhancer-less promoters. The "new" Luci-TRE-SMN(B) transgene was microinjected into the male pronucleus of FVB/N embryos. Founder mice were bred with Camk2a-tTA mice (FVB background; see Stock No. 003010) to determine which lines had high dox-inducible levels of luciferase and SMN in brain. The high-expressing "new" Luci-TRE-SMN(B) founder line was identified and used to create the Tg(SMN2)89; Smn1^+/-; Tg(SMNΔ7)4299; ROSA26^rtTA; "new" Luci-TRE-SMN(B) mice (on a mixed FVB/N and C57BL/6 genetic background) that was sent to The Jackson Laboratory Repository as Stock No. 017597). Upon arrival, some mice were bred with FVB/NJ inbred mice (Stock No. 001800) for several generations to remove the other transgenes/mutations. The resulting mice harboring only the "new" Luci-TRE-SMN(B) transgene were assigned Stock No. 017600.

Expression Data

Expressed Gene	SMN2, survival of motor neuron 2, centromeric, human
Expressed Gene	luc, luciferase, firefly
Site of Expression	When bred to mice expressing a tetracycline transactivator (tTA) or reverse transactivator (rtTA), doxycycline induction results in the expression of luciferase and SMN expression in transactivator expressing tissues of the offspring.

Control Suggestions

Noncarrier
001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Selected References

Le TT; McGovern VL; Alwine IE; Wang X; Massoni-Laporte A; Rich MM; Burghes AH. 2011. Temporal requirement for high SMN expression in SMA mice. Hum Mol Genet 20(18):3578-91. PubMed: 21672919 MGI: J:174960

View All References

Genetics

Tg(tetO-SMN2,-luc)#bAhmb

Allele Symbol: Tg(tetO-SMN2,-luc)#bAhmb

Allele Name	transgene insertion, Arthur H M Burghes
Allele Type	Transgenic (Humanized sequence, Inducible, Inserted expressed sequence, Reporter)
Allele Synonym(s)
Gene Symbol and Name	Tg(tetO-SMN2,-luc)#bAhmb, transgene insertion, Arthur H M Burghes
Gene Synonym(s)
Promoter	tetO, tet operator,
Expressed Gene	SMN2, survival of motor neuron 2, centromeric, human
Expressed Gene	luc, luciferase, firefly
Site of Expression	When bred to mice expressing a tetracycline transactivator (tTA) or reverse transactivator (rtTA), doxycycline induction results in the expression of luciferase and SMN expression in transactivator expressing tissues of the offspring.
Strain of Origin	FVB/N
Chromosome	UN
Molecular Note	The tetracycline response element with two minimal CMV promoters drives inducible expression of human SMN (exons 1 through 8) and a luciferase gene.
Mutations Made By	Arthur Burghes, The Ohio State University

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Neurodevelopmental Defects
Neurobiology Research
- Ataxia (Movement) Defects
- Fluorescent protein expression in neural tissue
- Neurodegeneration
- Spinal Muscular Atrophy (SMA)
- Tet Expression System
  - tTA/rtTA Responsive Strains
Research Tools
- Genetics Research
  - Tissue/Cell Markers
  - Tissue/Cell Markers: multiple
  - Tissue/Cell Markers: neurons
- Neurobiology Research
  - Tetop Tet System
  - cell marker
- Tet Expression Systems
  - tTA/rtTA Responsive Strains

References

Le TT; McGovern VL; Alwine IE; Wang X; Massoni-Laporte A; Rich MM; Burghes AH. 2011. Temporal requirement for high SMN expression in SMA mice. Hum Mol Genet 20(18):3578-91. PubMed: 21672919 MGI: J:174960

Technical Support

Contact Technical Support

Genotyping Protocols
- MELT: Grm7^{Tg(SMN2)89Ahmb} Alternate1
- Separated MCA: Tg(tetO-SMN,-luc)#Ahmb
- Separated PCR: Tg(tetO-SMN,-luc)#Ahmb
- High Resolution Melting: Tg(tetO-SMN,-luc)#Ahmb
- Genotyping resources and troubleshooting
Breeding Considerations

Some Tg(SMN2)89; Smn1^+/-; Tg(SMNΔ7)4299; ROSA26^rtTA; "new" Luci-TRE-SMN(B) mice (Stock No. 017597) were bred with FVB/NJ inbred mice (Stock No. 001800) for several generations, selecting for the "new" Luci-TRE-SMN(B) transgene and to remove the other transgenes/mutations. The resulting mice harboring only the "new" Luci-TRE-SMN(B) transgene were assigned Stock No. 017600. When maintaining the live "new" Luci-TRE-SMN(B) transgenic colony, transgenic mice may be bred with wildtype (noncarrier) mice from the colony or with FVB/NJ inbred mice (Stock No. 001800).
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Tg(tetO-SMN2,-luc)#bAhmb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017600 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Hemizygous or Non Carrier for Tg(tetO-SMN2,-luc)#bAhmb

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(tetO-SMN2,-luc)#bAhmb

Allele Symbol: Tg(tetO-SMN2,-luc)#bAhmb

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

References

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability