Overview

These Tg(SMN2)89; Smn1^+/-; Tg(SMNΔ7)4299; ROSA26^rtTA; "old" Luci-TRE-SMN mice allow high levels of both luciferase and full-length human SMN expression to be regulated by the addition/removal of doxycycline. These may be useful as a fluorescent reporter and/or Tet-On/Tet-Off tool strain for doxycycline-inducible rescue of Type II (moderate) proximal spinal muscular atrophy (SMA).

Donating Investigator

Arthur H.M. Burghes, The Ohio State University

Genetic overview

Genetic Background	Generation

Tg(SMN2*delta7)4299Ahmb

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

Grm7^{Tg(SMN2)89Ahmb}

Allele Type
Transgenic (Hypomorph, Inserted expressed sequence, Humanized sequence)

Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy}

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Transactivator)	Gt(ROSA)26Sor	gene trap ROSA 26, Philippe Soriano

Smn1^tm1Msd

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Smn1	survival motor neuron 1

Tg(tetO-SMN2,-luc)#aAhmb

Allele Type
Transgenic (Reporter, Inducible, Inserted expressed sequence, Humanized sequence)

View Genetics

Research Applications

Research Tools
Neurobiology Research
Developmental Biology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

These Tg(SMN2)89; Smn1^+/-; Tg(SMNΔ7)4299; ROSA26^rtTA; "old" Luci-TRE-SMN mice harbor multiple mutations/transgenes.

The moderate Type II SMA mouse model (see Stock No. 005025) is defined by mice homozygous for the Tg(SMN2)89 transgene (SMN2+/+), homozygous for the Smn1^tm1Msd mutation (Smn1^-/-), and homozygous for the Tg(SMNΔ7)4299 transgene (SMNΔ7+/+). At birth, SMN2+/+; Smn1^-/-; SMNΔ7+/+ mice are small with neuromuscular defects by 5 days old that progress to abnormal gait, hind limb weakness/immobility, and eventually death at approximately 13 days of age.

The ROSA26^rtTA targeted mutation results in widespread reverse tetracycline transactivator (rtTA)-expression. The Luci-TRE-SMN transgene has expression of both luciferase and a full-length (exons 1-8) human SMN under the control of a bi-directional tet-responsive promoter. When together in mice, addition of doxycycline (dox) results in widespread and simultaneous expression of luciferase and full-length human SMN expression. When doxycycline (dox) food is introduced to the dams at pup birth, neonatal brain and spinal cord exhibit high levels of luciferase activity beginning at ~48 hours post-induction and increasing to maximum after 10 days on dox. Similarly, full-length SMN is induced at 3 days post-induction and continues to increase to 10 days post-induction (in a similar manner to endogenous SMN protein). Similar results are observed following dox food administration to 28 day old mice. Following 10 days of dox, western blot shows luciferase and human SMN expression in widespread tissues including muscle, brain, liver, heart, spinal cord, heart, kidney, lung, and spleen. In addition, dox induction results in increased SMN expression in motor neurons as well as gems within those motor neurons. Following 28 days of dox administration, the subsequent removal of dox for 10 days results in luciferase and SMN expression levels dropping to non-induced levels.

These Tg(SMN2)89; Smn1^+/-; Tg(SMNΔ7)4299; ROSA26^rtTA; "old" Luci-TRE-SMN mice allow high levels of both luciferase and full-length human SMN expression to be regulated by the addition/removal of dox. Mice heterozygous for the Smn1^tm1Msd mutation (Smn1^+/-) and homozygous for the other mutations/transgenes are viable and fertile with no reported abnormalities or symptoms of neuropathology. The phenotype of mice homozygous for the Tg(SMN2)89 transgene (SMN2+/+), homozygous for the Smn1^tm1Msd mutation (Smn1^-/-), homozygous for the Tg(SMNΔ7)4299 transgene (SMNΔ7+/+), homozygous for the ROSA26^rtTA mutation (ROSA26^rtTA+/+), and hemizygous or homozygous for the Luci-TRE-SMN is described below.

In the absence of dox, the SMN2+/+; Smn1^-/-; SMNΔ7+/+; ROSA26^rtTA+/+; Luci-TRE-SMN mice (called non-induced SMA mice) die on average of 13 days old with no extension of lifespan (no mice live past ~18 days old). As expected, this is the same phenotype as moderate Type II SMA mouse model (see Stock No. 005025).

When pregnant dams are given dox beginning coincident with gestating pup embryonic day (E)13, the high levels of full-length human SMN expression over the early postnatal period in SMN2+/+; Smn1^-/-; SMNΔ7+/+; ROSA26^rtTA+/+; Luci-TRE-SMN mice results in substantial rescue of SMA: the mice have no marked motor deficits, near normal motor neuron electrophysiology/neuromuscular junction (NMJ) function, fully developed NMJs, and average lifespan of 132 days (~30% of mice live over 200 days). Additionally, early postnatal SMN induction followed by removal of dox from 28-58 days of age, result in no morphological or electrophysiological abnormalities at the NMJ and no overt motor phenotype. Later dox administration to nursing dams rescues SMA but to a lesser extent: dox treatment to nursing dams at pup birth/postnatal day 1 (P0/P1) results in average lifespan of 86 days (~15% of mice live over 200 days), while dox treatment to nursing dams at pup postnatal day 2 (P2) leads to average lifespan of 25 days.

The donating investigator reports the following doxycycline approach. For embryonic induction, pregnant females were given water containing 500 mg/ml of doxycycline. At pup birth, doxycycline food (200 mg/kg) was introduced to the cage and the doxycycline water removed. For postnatal induction, high-concentration doxycycline food (6 g/kg) was administered to the mother; thus the pups received doxycycline in the mother's milk. Of note, high-concentration doxycycline food (6 g/kg) was found to severely affect birth rates if administered to pregnant mice.

Development

These Tg(SMN2)89; Smn1^+/-; Tg(SMNΔ7)4299; ROSA26^rtTA; "old" Luci-TRE-SMN mice harbor several mutations/transgenes, as described below.

Mice homozygous for the Tg(SMN2)89 transgene (SMN2+/+), homozygous for the Smn1^tm1Msd mutation (Smn1^-/-), and homozygous for the Tg(SMNΔ7)4299 transgene (SMNΔ7+/+) define the moderate type II SMA mouse model. The Tg(SMN2)89; Smn1^-/-; Tg(SMNΔ7)4299 mice are described as Stock No. 005025. These mice were bred to and maintained upon an FVB/N genetic background prior to being used for breeding as below.

To generate the widespread rtTa-expressing mutation ROSA26^rtTA, mice with the flox-STOP ROSA26-rtTA mutation (ICR;129 background; Stock No. 005572) were bred with Sox2-Cre transgenic mice (FVB;Swiss Webster;C57BL6;CBA background; see Stock No. 004783). The resulting offspring with pan-deletion of the lox-flanked STOP cassette/widespread expression of rtTA were bred to and maintained upon a C57BL/6 genetic background prior to being used as a donor for the Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy} allele (see below).

The Luci-TRE-SMN transgene was created by Dr. Arthur HM Burghes (The Ohio State University). The Luci-TRE-SMN transgenic construct used here ["old" pBI-L-SMN] was designed with a luciferase sequence on one side, and a full-length human SMN2 cDNA sequence (encoding exons 1-8) on the other side of the P_bi-1 bi-directional tetracycline-responsive promoter. The 1.37 kb human SMN2 cDNA sequence was derived via RT-PCR performed on total RNA isolated from SMA type 1-derived patient fibroblast cells (Coriell Institute GM 3813; shown to express both full-length and truncated SMN2 transcripts). Compared to the "new" pBI-L-SMN(B) construct, the "old" pBI-L-SMN construct used here retains part of a hemagglutinin epitope tag sequence between the P_bi-1 promoter and 5' end of SMN2 cDNA. The P_bi-1 promoter contains the Tet-responsive element (TRE; seven copies of the 42-bp tet operator sequence [tetO]) placed between two minimal CMV enhancer-less promoters. The "old" Luci-TRE-SMN transgene was microinjected into the male pronucleus of FVB/N embryos. Founder mice were bred with Camk2a-tTA mice (FVB background; see Stock No. 003010) to determine which lines had high dox-inducible levels of luciferase and SMN in brain. The high-expressing "old" Luci-TRE-SMN founder line was identified and used as below.

To generate the Tg(SMN2)89; Smn1^+/-; Tg(SMNΔ7)4299; ROSA26^rtTA; "old" Luci-TRE-SMN animals, mice from the moderate type II SMA colony (Tg(SMN2)89+/+; Smn1^+/-; Tg(SMNΔ7)4299+/+) were bred to ROSA26^rtTA mice. Because the ROSA26^rtTA mutation and Tg(SMN2)89 transgene insertion lie relatively close to each other on chromosome 6, additional crosses were performed to FVB/N wildtype mice; and a single mouse with a recombinant chromosome containing both Tg(SMN2)89 and ROSA26^rtTA was identified. Next, those mice were bred to also harbor the "old" Luci-TRE-SMN transgene. The resulting Tg(SMN2)89; Smn1^+/-; Tg(SMNΔ7)4299; ROSA26^rtTA; "old" Luci-TRE-SMN mice (on a mixed FVB/N and C57BL/6 genetic background) were sent to The Jackson Laboratory Repository as Stock No. 017596. Upon arrival, males were used to cryopreserve sperm. To establish the living mouse colony, an aliquot of the frozen sperm was used to fertilize oocytes from moderate type II SMA females (Stock No. 005025).

Expression Data

Expressed Gene	SMN2, survival of motor neuron 2, centromeric, human
Site of Expression	Tg(SMN2*delta7)4299Ahmb
Expressed Gene	SMN2, survival of motor neuron 2, centromeric, human
Site of Expression	Grm7^{Tg(SMN2)89Ahmb}
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	Widespread reverse tetracycline transactivator (rtTA)-expression.
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	The expression of the lacZ gene in tissues where Smn is normally expressed was noted.
Expressed Gene	SMN2, survival of motor neuron 2, centromeric, human
Expressed Gene	luc, luciferase, firefly
Site of Expression	Following 10 days of dox, western blot shows luciferase and human SMN expression in widespread tissues including muscle, brain, liver, heart, spinal cord, heart, kidney, lung, and spleen. In addition, dox induction results in increased SMN expression in motor neurons as well as gems within those motor neurons.

Control Suggestions

The following strain(s) may also be appropriate experimental controls:
--mice from the moderate type II SMA strain (Stock No. 005025).

Approximate Controls

001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Selected References

Le TT; McGovern VL; Alwine IE; Wang X; Massoni-Laporte A; Rich MM; Burghes AH. 2011. Temporal requirement for high SMN expression in SMA mice. Hum Mol Genet 20(18):3578-91PubMed: 21672919 MGI: J:174960

View All References

Genetics

Tg(SMN2*delta7)4299Ahmb

Allele Symbol: Tg(SMN2*delta7)4299Ahmb

Allele Name	transgene insertion 4299, Arthur H M Burghes
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	SMNdelta7; Tg(SMN1*delta7)4299Ahmb
Gene Symbol and Name	Tg(SMN2*delta7)4299Ahmb, transgene insertion 4299, Arthur H M Burghes
Gene Synonym(s)
Promoter	SMN2, survival of motor neuron 2, centromeric, human
Expressed Gene	SMN2, survival of motor neuron 2, centromeric, human
Site of Expression	Tg(SMN2*delta7)4299Ahmb
Strain of Origin	FVB/N
Chromosome	UN
Molecular Note	The transgene contains a human SMN2 promoter and a human SMN2 cDNA (SMNdelta7) that lacks exon 7. There are 14 copies of this allele integrated into the genome.
Mutations Made By	Arthur Burghes, The Ohio State University

Grm7^{Tg(SMN2)89Ahmb}

Allele Symbol: Grm7^{Tg(SMN2)89Ahmb}

Allele Name	transgene insertion 89, Arthur H M Burghes
Allele Type	Transgenic (Hypomorph, Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	SMN2
Gene Symbol and Name	Grm7, glutamate receptor, metabotropic 7
Gene Synonym(s)
Promoter	SMN2, survival of motor neuron 2, centromeric, human
Expressed Gene	SMN2, survival of motor neuron 2, centromeric, human
Site of Expression	Grm7^{Tg(SMN2)89Ahmb}
Strain of Origin	FVB/N
Chromosome	6
Molecular Note	A 35.5 kb genomic fragment containing the human survival motor neuron 2 (SMN2) gene and promoter was used for the transgene. The transgene is ubiquitously expressed in all tissues examined by Northern blot analysis. Line 89 carries 1 copy of the transgene integrated into intron 4 of the gene. RT-PCR confirmed reduced expression of the gene the transgene is integrated into.
Mutations Made By	Arthur Burghes, The Ohio State University

Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy}

Allele Symbol: Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy}

Allele Name	targeted mutation 1.1, Andras Nagy
Allele Type	Targeted (Reporter, Transactivator)
Allele Synonym(s)	ROSA26rtTA
Gene Symbol and Name	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Gene Synonym(s)
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	Widespread reverse tetracycline transactivator (rtTA)-expression.
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	6
Molecular Note	A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Cre-mediated recombination removed the floxed neo cassette allowing the ROSA26 promoter to drive expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene.

Smn1^tm1Msd

Allele Symbol: Smn1^tm1Msd

Allele Name	targeted mutation 1, Michael Sendtner
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	SMN^-
Gene Symbol and Name	Smn1, survival motor neuron 1
Gene Synonym(s)
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	The expression of the lacZ gene in tissues where Smn is normally expressed was noted.
Strain of Origin	129P2/OlaHsd
Chromosome	13
Molecular Note	A lacZ-neo cassette was inserted into exon 2 by homologous recombination resulting in an in-frame fusion of lacZ to exon 2. Homozygous mutant embryos were identified up to 80 hours post coitum. The expression of the lacZ gene in tissues where Smn is normally expressed was noted.
Mutations Made By	Michael Sendtner

Tg(tetO-SMN2,-luc)#aAhmb

Allele Symbol: Tg(tetO-SMN2,-luc)#aAhmb

Allele Name	transgene insertion, Arthur H M Burghes
Allele Type	Transgenic (Reporter, Inducible, Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	Luci-TRE-SMN
Gene Symbol and Name	Tg(tetO-SMN2,-luc)#aAhmb, transgene insertion, Arthur H M Burghes
Gene Synonym(s)
Promoter	tetO, tet operator,
Expressed Gene	SMN2, survival of motor neuron 2, centromeric, human
Expressed Gene	luc, luciferase, firefly
Site of Expression	Following 10 days of dox, western blot shows luciferase and human SMN expression in widespread tissues including muscle, brain, liver, heart, spinal cord, heart, kidney, lung, and spleen. In addition, dox induction results in increased SMN expression in motor neurons as well as gems within those motor neurons.
Strain of Origin	FVB/N
Chromosome	UN
Molecular Note	The tetracycline response element with two minimal CMV promoters drives inducible expression of human SMN (exons 1 through 7) and a luciferase gene. Two lines were generated. The pound symbol (#) is used when no line is specified and/or lines are pooled.
Mutations Made By	Arthur Burghes, The Ohio State University

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Tet Expression Systems
  - tTA/rtTA Expressing Strains
  - tTA/rtTA Responsive Strains
- Neurobiology Research
  - cell marker
  - Tetop Tet System
- Genetics Research
  - Tissue/Cell Markers: neurons
  - Mutagenesis and Transgenesis: Tetop Tet System
  - Tissue/Cell Markers
  - Tissue/Cell Markers: multiple
- Fluorescent Proteins
Neurobiology Research
- Tet Expression System
  - tTA/rtTA Expressing Strains
  - tTA/rtTA Responsive Strains
- Ataxia (Movement) Defects
- Spinal Muscular Atrophy (SMA)
- Fluorescent protein expression in neural tissue
- Neurodegeneration
Developmental Biology Research
- Neurodevelopmental Defects

Genotype: Smn1^tm1Msd related

Neurobiology Research
- Spinal Muscular Atrophy (SMA)

Mammalian Phenotype Terms by Genotype

References

Le TT; McGovern VL; Alwine IE; Wang X; Massoni-Laporte A; Rich MM; Burghes AH. 2011. Temporal requirement for high SMN expression in SMA mice. Hum Mol Genet 20(18):3578-91PubMed: 21672919 MGI: J:174960

Additional - Grm7^{Tg(SMN2)89Ahmb} related

Additional - Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy} related

Additional - Smn1^tm1Msd related

Additional - Tg(SMN2*delta7)4299Ahmb related

Additional - Tg(tetO-SMN2,-luc)#aAhmb related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tg(SMN2*)
- Standard PCR:Smn1
- Separated MCA:Tg(tetO-SMN,-luc)#Ahmb
- Separated PCR:Smn1
- Separated PCR:Gt(ROSA)26Sor
- Separated MCA:Gt(ROSA)26Sor
- Standard PCR:Tg(tetO-SMN,-luc)#Ahmb
- Separated PCR:Tg(tetO-SMN,-luc)#Ahmb
- QPCR:Tg(SMN2*)
- Genotyping resources and troubleshooting
Breeding Considerations

For routine maintenance of the live colony, mice of the following genotype may be bred together: homozygous for Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy}, heterozygous or homozygous for Smn1^tm1Msd, homozygous for Tg(SMN2)89, homozygous for the Tg(SMNΔ7)4299, and hemizygous or homozygous for Tg(tetO-SMN2,-luc)#a.
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy} Grm7^{Tg(SMN2)89Ahmb} Smn1^tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#aAhmb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017596 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	all 5 genes must be represented

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Tg(SMN2*delta7)4299Ahmb

Allele Symbol: Tg(SMN2*delta7)4299Ahmb

Grm7Tg(SMN2)89Ahmb

Allele Symbol: Grm7Tg(SMN2)89Ahmb

Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy

Allele Symbol: Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy

Smn1tm1Msd

Allele Symbol: Smn1tm1Msd

Tg(tetO-SMN2,-luc)#aAhmb

Allele Symbol: Tg(tetO-SMN2,-luc)#aAhmb

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Smn1tm1Msd related

Mammalian Phenotype Terms by Genotype

References

Additional - Grm7Tg(SMN2)89Ahmb related

Additional - Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy related

Additional - Smn1tm1Msd related

Additional - Tg(SMN2*delta7)4299Ahmb related

Additional - Tg(tetO-SMN2,-luc)#aAhmb related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Grm7^{Tg(SMN2)89Ahmb}

Allele Symbol: Grm7^{Tg(SMN2)89Ahmb}

Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy}

Allele Symbol: Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy}

Smn1^tm1Msd

Allele Symbol: Smn1^tm1Msd

Genotype: Smn1^tm1Msd related

Additional - Grm7^{Tg(SMN2)89Ahmb} related

Additional - Gt(ROSA)26Sor^{tm1.1(rtTA,EGFP)Nagy} related

Additional - Smn1^tm1Msd related