In this strain, a monomeric red fluorescent protein (RFP) sequence replaces the coding sequence of the chemokine (C-C motif) receptor 2 (Ccr2) gene, abolishing gene function. These mice may be useful for studying the role of CCR2 during monocyte recruitment to sites of inflammation.
Israel F. Charo, Gladstone Inst of Cardiovascular Disease, UCSF
Genetic Background | Generation |
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N9pN1+N2F2
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Ccr2 | chemokine (C-C motif) receptor 2 |
In this strain, a monomeric red fluorescent protein (RFP) sequence replaces the coding sequence of the chemokine (C-C motif) receptor 2 (Ccr2) gene, abolishing gene function. CCR2 is a chemokine receptor expressed on monocytes which directs their recruitment to sites of inflammation found in diseases such as multiple sclerosis, atherosclerosis, rheumatoid arthritis, and experimental autoimmune encephalitis (EAE), following activation by monocyte chemoattractant proteins (MCPs). CCR2 is essential to innate and adaptive immune responses during injury repair. Homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. In these mice RFP expression is seen in circulating monocytes, T cells, and natural killer cells. They exhibit a reduction in monocyte recruitment during thioglycolate-induced peritonitis and EAE. These mice may be useful for studying the role of CCR2 during monocyte recruitment to sites of inflammation.
A targeting vector was designed to replace the coding sequence of the chemokine (C-C motif) receptor 2 (Ccr2) gene with a monomeric red fluorescent protein (RFP) sequence followed by a polyadenylation signal and a loxP-flanked neomycin resistance cassette. The construct was electroporated into 129-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. The resulting offspring were bred to Tg(EIIa-cre)C5379Lmgd transgenic mice to delete the neo cassette. The donating investigator reported that these mice were then backcrossed to C57BL/6J mice for at least 9 generations (see SNP note below). Upon arrival, mice were bred to C57BL/6J mice (Stock No. 000664) for at least one generation.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression | The red fluorescent protein (RFP) reporter allows visualization of Ly6Chi monocytes. |
Allele Name | targeted mutation 2.1, Israel F Charo |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Ccr2R; Ccr2RFP |
Gene Symbol and Name | Ccr2, chemokine (C-C motif) receptor 2 |
Gene Synonym(s) | |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | The red fluorescent protein (RFP) reporter allows visualization of Ly6Chi monocytes. |
Strain of Origin | 129 |
Chromosome | 9 |
Molecular Note | The coding region was replaced with an RFP and floxed neo cassette. Cre-mediated recombination removed the neo cassette. The absence of transcript expression was confirmed by RT-PCR. |
Mutations Made By | Israel Charo, Gladstone Inst of Cardiovascular Disease, UCSF |
When maintaining a live colony, homozygous mice may be bred together.
When using the Ccr2RFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #017586 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ccr2<tm2.1Ifc> |
Frozen Mouse Embryo | B6.129(Cg)-Ccr2<tm2.1Ifc>/J | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Ccr2<tm2.1Ifc>/J | $2595.00 |
Frozen Mouse Embryo | B6.129(Cg)-Ccr2<tm2.1Ifc>/J | $3373.50 |
Frozen Mouse Embryo | B6.129(Cg)-Ccr2<tm2.1Ifc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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