Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes have an embryonic lethal phenotype.
Although homozygous embryos are similar in size to wildtype controls at E3.5, by E4.5 homozygous embryos exhibit delayed growth. At E5.5, homozygous embryos are completely resorbed or reduced in size with abnormal morphology. Cultured homozygous blastocysts exhibit cell cycle arrest at prometaphase of mitosis and increased apoptosis.
A targeting vector containing a PGK-puromycin cassette was used to disrupt exons 3 through 14. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1, Piotr Sicinski|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||E4f1, E4F transcription factor 1|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Exons 3-14 were replaced with a puromycin resistance gene. The removed sequence encodes amino acids 104-784, including all the C2H2 zinc-finger domains responsible for DNA binding and functional activities. Southern blotting confirmed recombination.|
|Mutations Made By|| |
Laurent Le Cam, INSERM
When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have an embryonic lethal phenotype.
When using the B6;129S4-E4f1tm1Pisc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017583 in your Materials and Methods section.