These mice possess loxP sites flanking exons 4-14 of the E4F transcription factor 1 ( E4f1) gene and have applications in studies related to cell cycle regulation and cell proliferation and survival.
Laurent Le Cam, INSERM
These mice possess loxP sites on either side of exons 4 through 14, of the targeted gene. E4f1 is a multifunctional transcription factor with widespread expression and is involved in signaling pathways that regulate cell proliferation and survival, and tumorigenesis. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 through 14 deleted in the cre-expressing tissue(s).
A loxP site flanked targeting vector containing an FRT-site flanked hygromycin-TK cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 14 and the polyA signal of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a FLP recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1.1, Laurent Le Cam|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||E4f1, E4F transcription factor 1|
|Site of Expression||E4f1 inactivation in the entire skin or in the basal compartment of the epidermis induces skin homeostasis defects and E4f1 depletion results in exhaustion of the epidermal stem cell pool.|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A loxP site was inserted upstream of exon 4 and a second loxP site followed by an FRT flanked hygromycin thymidine kinase cassette were inserted downstream of exon 14 via homologous recombination. Flp mediated recombination removed the hygromycin thymidine kinase cassette.|
|Mutations Made By|| |
Laurent Le Cam, INSERM
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129S4-E4f1tm1.1Llca/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017568 in your Materials and Methods section.