B6;129S4-E4f1^tm1.1Llca/J

Stock number: 017568
Research areas: Research Tools

Description

These mice possess loxP sites flanking exons 4-14 of the E4F transcription factor 1 ( E4f1) gene and have applications in studies related to cell cycle regulation and cell proliferation and survival.

Detailed description

These mice possess loxP sites on either side of exons 4 through 14, of the targeted gene. E4f1 is a multifunctional transcription factor with widespread expression and is involved in signaling pathways that regulate cell proliferation and survival, and tumorigenesis. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 through 14 deleted in the cre-expressing tissue(s).

Development

A loxP site flanked targeting vector containing an FRT-site flanked hygromycin-TK cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 14 and the polyA signal of the targeted gene, and another loxP site was inserted upstream of exon 4. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a FLP recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: E4f1^tm1.1Llca
Name: targeted mutation 1.1, Laurent Le Cam
MGI accession ID: MGI:4867860
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: E4f1
Marker name: E4F transcription factor 1
Chromosome: 17
Strain of origin: 129S4/SvJae
Site of expression: E4f1 inactivation in the entire skin or in the basal compartment of the epidermis induces skin homeostasis defects and E4f1 depletion results in exhaustion of the epidermal stem cell pool.
Molecular note: A loxP site was inserted upstream of exon 4 and a second loxP site followed by an FRT flanked hygromycin thymidine kinase cassette were inserted downstream of exon 14 via homologous recombination. Flp mediated recombination removed the hygromycin thymidine kinase cassette.