These floxed mutant mice possess loxP sites flanking exons 5 and 6 of the Fbw7 gene. This strain may be useful in generating conditional mutants to study ubiquitin ligase, hematopoietic stem cell self-renewal/differentiation and tumor suppression.
Iannis Aifantis, NYU School of Medicine
Genetic Background | Generation |
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?+pN1
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Fbxw7 | F-box and WD-40 domain protein 7 |
Starting at:
$255.00 Domestic price for female 4-week |
$2,854.50 Domestic price Cryo Recovery |
These Fbw7f/f mice possess loxP sites flanking exons 5 and 6. These exons encode the F-box domain required for ubiquitination complex assembly. As a ubiquitin ligase, Fbwx7 is involved in targeting the cell cycle regulators c-Myc and Notch1 for proteasomal degradation. Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study regulation of hematopoietic stem cells and tumor suppression.
For example, when crossed to a strain expressing interferon-inducible Cre recombinase (see Stock No. 003556), mice exhibit impaired hematopoietic stem cell self-renewal and differentiation.
A targeting vector was designed to place a loxP site upstream of exon 5 and an FRT-flanked neomycin resistance cassette followed by a loxP site downstream of exon 6. The construct was electroporated into 129/SvEn x C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Strong chimeras were identified and crossed to C57BL/6 females to verify germline transmission. The F1 generation was PCR screened and crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (Stock No. 003946) to remove the neo cassette and then further backcrossed to C57BL/6 for at least three generations. At some point, the Fbw7f colony was bred to Mx1-Cre mice (see Stock No. 003556). In 2012, the Fbw7f/f mice (B6;129 genetic background) were sent to The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J for at least one generation to establish our Fbw7f colony. The Cre-expressing allele was bred away from our live colony.
Allele Name | targeted mutation 1, Iannis Aifantis |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Fbw7flox |
Gene Symbol and Name | Fbxw7, F-box and WD-40 domain protein 7 |
Gene Synonym(s) | |
Strain of Origin | 129/Sv x C57BL/6 |
Chromosome | 3 |
Molecular Note | A loxP site was inserted downstream of exon 4 and an frt- and loxP-flanked neo cassette was inserted downstream of exon 6. Germ line, frt-mediated recombination was used to remove the neo cassette leaving exons 5 and 6 floxed. |
Mutations Made By | Iannis Aifantis, NYU School of Medicine |
While maintaining a live colony, these mice are bred as homozygotes.
When using the Fbw7flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #017563 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Fbxw7<tm1Iaai> |
Frozen Mouse Embryo | B6;129-Fbxw7<tm1Iaai>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Fbxw7<tm1Iaai>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Fbxw7<tm1Iaai>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Fbxw7<tm1Iaai>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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