These aP2-hIKKbeta SE transgenic mice express a constitutively active form of the human inhibitor of kappaB kinase beta (IKBKB) gene in adipose tissue under direction of the mouse fatty acid binding protein 4 (Fabp4) promoter. These mice may be useful for studying the effect of inflammation in adipose tissue on metabolism.
Haiyan Xu, Rhode Island Hospital, Warren Alpert Medical School of Brown University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Constitutively active, Inserted expressed sequence, Humanized sequence) |
These aP2-hIKKbeta SE transgenic mice express a constitutively active form of the human inhibitor of kappaB kinase beta (IKBKB) gene in adipose tissue under direction of the mouse fatty acid binding protein 4 (Fabp4) promoter. Hemizygous mice are viable and fertile. IKBKB gene product, IKKβ, is a master kinase involved in obesity-related inflammation and insulin resistance. These mice exhibit increased food intake, reduced weight of white adipose tissue, increased energy expenditure, and reduced triglyceride contents in adipose tissue, liver and muscle. They also exhibit reduced plasma free fatty acid (FFA) levels, increased systemic and tissue inflammation, decreased blood glucose levels, and improved glucose and insulin tolerance. These aP2-hIKKbeta SE mice show a decrease in hepatosteatosis even on a high fat diet. These mice may be useful for studying the effects of inflammation on adipose tissue and metabolism.
The aP2-hIKKbeta SE transgene was designed with a constitutively active form of the human inhibitor of kappaB kinase beta (IKBKB) gene driven by a mouse fatty acid binding protein 4 (Fabp4) promoter, followed by an SV40 polyadenylation site. The transgene was microinjected into fertilized FVB/NJ oocytes, and mice from founder line 49 were bred to C57BL/6 mice for at least five generations to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | IKBKB, inhibitor of nuclear factor kappa B kinase subunit beta, human |
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Site of Expression |
Allele Name | transgene insertion 49, Haiyan Xu |
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Allele Type | Transgenic (Constitutively active, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | aP2-hIKKbeta SE |
Gene Symbol and Name | Tg(Fabp4-IKBKB*)49Hxu, transgene insertion 49, Haiyan Xu |
Gene Synonym(s) | |
Promoter | Fabp4, fatty acid binding protein 4, adipocyte, mouse, laboratory |
Expressed Gene | IKBKB, inhibitor of nuclear factor kappa B kinase subunit beta, human |
Strain of Origin | FVB/NJ |
Chromosome | UN |
Molecular Note | The aP2-hIKKbeta SE transgene was designed with a constitutively active form of the human inhibitor of kappaB kinase beta (IKBKB) gene driven by a mouse fatty acid binding protein 4 (Fabp4) promoter, followed by an SV40 polyadenylation site. Line 49 was generated. |
Mutations Made By | Haiyan Xu, Rhode Island Hospital, Warren Alpert Medical School of Brown University |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the B6.FVB-Tg(Fabp4-IKBKB*)49Hxu/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017560 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Fabp4-IKBKB*)49Hxu |
Frozen Mouse Embryo | B6.FVB-Tg(Fabp4-IKBKB*)49Hxu/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Fabp4-IKBKB*)49Hxu/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Fabp4-IKBKB*)49Hxu/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.FVB-Tg(Fabp4-IKBKB*)49Hxu/J Frozen Embryo | $3373.50 |
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