B6.FVB-Tg(Fabp4-IKBKB*)49Hxu/J

Stock number: 017560
Research areas: Diabetes and Obesity Research, Endocrine Deficiency Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research

Description

These aP2-hIKKbeta SE transgenic mice express a constitutively active form of the human inhibitor of kappaB kinase beta (IKBKB) gene in adipose tissue under direction of the mouse fatty acid binding protein 4 (Fabp4) promoter. These mice may be useful for studying the effect of inflammation in adipose tissue on metabolism.

Detailed description

These aP2-hIKKbeta SE transgenic mice express a constitutively active form of the human inhibitor of kappaB kinase beta (IKBKB) gene in adipose tissue under direction of the mouse fatty acid binding protein 4 (Fabp4) promoter. Hemizygous mice are viable and fertile. IKBKB gene product, IKKβ, is a master kinase involved in obesity-related inflammation and insulin resistance. These mice exhibit increased food intake, reduced weight of white adipose tissue, increased energy expenditure, and reduced triglyceride contents in adipose tissue, liver and muscle. They also exhibit reduced plasma free fatty acid (FFA) levels, increased systemic and tissue inflammation, decreased blood glucose levels, and improved glucose and insulin tolerance. These aP2-hIKKbeta SE mice show a decrease in hepatosteatosis even on a high fat diet. These mice may be useful for studying the effects of inflammation on adipose tissue and metabolism.

Development

The aP2-hIKKbeta SE transgene was designed with a constitutively active form of the human inhibitor of kappaB kinase beta (IKBKB) gene driven by a mouse fatty acid binding protein 4 (Fabp4) promoter, followed by an SV40 polyadenylation site. The transgene was microinjected into fertilized FVB/NJ oocytes, and mice from founder line 49 were bred to C57BL/6 mice for at least five generations to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.

Allele

Symbol: Tg(Fabp4-IKBKB*)49Hxu
Name: transgene insertion 49, Haiyan Xu
MGI accession ID: MGI:5293617
Generation method: Transgenic
Attributes: Constitutively active; Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Fabp4-IKBKB*)49Hxu
Marker name: transgene insertion 49, Haiyan Xu
Chromosome: UN
Strain of origin: FVB/NJ
Expressed genes: IKBKB,inhibitor of nuclear factor kappa B kinase subunit beta,human
Molecular note: The aP2-hIKKbeta SE transgene was designed with a constitutively active form of the human inhibitor of kappaB kinase beta (IKBKB) gene driven by a mouse fatty acid binding protein 4 (Fabp4) promoter, followed by an SV40 polyadenylation site. Line 49 was generated.