These mice express Cre recombinase under the control of the human bestrophin 1 (BEST) promoter. This strain represents an effective tool for generating retinal pigment epithelium (RPE) specific-targeted mutants that would be useful in studies of human retinopathies.
Joshua L. Dunaief, University of Pennsylvania
Genetic Background | Generation |
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?+pN1
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Allele Type |
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Transgenic (Recombinase-expressing) |
These mice express Cre recombinase under the control of the human bestrophin 1 (BEST) promoter. Mosaic Cre recombinase expression (mRNA) is seen in 50-90% of retinal pigment epithelium (RPE) cells, with some expression seen in the testis. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in RPE cells. Homozygotes are viable and fertile. This strain represents an effective tool for generating RPE specific-targeted mutants that would be useful in studies of human retinopathies.
When bred to a strain expressing a floxed-Dicer1 gene (see Dicer1tm1Bdh Stock No. 006366 for example), this mutant mouse strain may be useful for studying age-related macular degeneration.
A transgenic construct containing cre coding sequence under the control of the human bestrophin 1 (BEST) promoter was introduced into C57BL/6NCrl donor oocytes. Resulting offspring, containing 20 copies of the transgene, were bred to and further maintained on a C57BL/6J background. The transgene inserted 58 kb upstream of Tm108 on chromosome 9, the integration site contains a 120 bp deletion. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | The BEST1-cre line with expression in the highest percentage of retinal pigment epithelium (RPE) cells displayed a patchy mosaic expression pattern, with 50% to 90% of RPE cells expressing cre. |
Allele Name | transgene insertion 1, Joshua L Dunaief |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | BEST1-cre |
Gene Symbol and Name | Tg(BEST1-cre)1Jdun, transgene insertion 1, Joshua L Dunaief |
Gene Synonym(s) | |
Promoter | BEST1, bestrophin 1, human |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | The BEST1-cre line with expression in the highest percentage of retinal pigment epithelium (RPE) cells displayed a patchy mosaic expression pattern, with 50% to 90% of RPE cells expressing cre. |
Strain of Origin | C57BL/6 |
Chromosome | 9 |
Molecular Note | A fragment of the human BEST1 promoter (nucleotides -585 to +38) drives cre recombinase cDNA, the SV40 t-antigen intron, and HSV-TK polyA in the construct. This was purified and microinjected into C57BL/6 zygotes. Six founders were generated with one F2 line that showed the highest percentage of cre immunoreactivity in the RPE was selected for further study. Expression of cre was mosaic, with 50-90% of RPE cells being labeled. This line has a transgene copy number of 20 determined by qPCR analysis (J:170522) or 5 copies (J:240920) inserted about 58 kb upstream of Tmem108 (chr9:103,722,073; NCBI37/mm9) with a 120 bp deletion at the transgene integration site. |
Mutations Made By | Joshua Dunaief, University of Pennsylvania |
When maintaining a live colony, homozygous mice may be bred together.
When using the Best1-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #017557 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(BEST1-cre)1Jdun |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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