In this B7-DC KO strain, an enhanced green fluorescent protein (EGFP) replaces exon 2, including the initiation codon, of the programmed cell death 1 ligand 2 gene (Pdcd1lg2) - abolishing endogenous gene function. These mice may be useful for studying self tolerance, Th1 and Th2 immune responses.
Drew Pardoll, Johns Hopkins University School of Medic
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Pdcd1lg2 | programmed cell death 1 ligand 2 |
In this strain, an enhanced green fluorescent protein (EGFP) and a loxP-flanked neomycin resistance (neo) cassette replace exon 2, including the initiation codon, of the programmed cell death 1 ligand 2 (Pdcd1lg2) gene, abolishing gene function. Homozygotes are viable and fertile. The Pdcd1lg2 gene encodes the costimulatory molecule B7-DC which is expressed in dendritic cells (DCs). B7-DC binds to the PD-1 immunoreceptor tyrosine-based inhibitory motif-containing receptor expressed on activated T cells where it regulates self tolerance and immune responses to microbes. These mice exhibit a reduction of intrahepatic tumor-specific CD8 T cells, interferon-γ (IFN-γ) production by CD4 T cells, IFN-γ-dependent humoral responses , antigen-specific CD8 T cell responses, and cytotoxic T lymphocyte (CTL) activity. In these mice hepatic tumors grow more quickly than in wildtype mice. GFP expression is not visible in B7-DC tissue, but the donating investigator reports that GFP expression can be observed in cultured DCs and macrophages when treated with interleukin (IL)-4 or IL-13. These mice may be useful for studying self tolerance, Th1 and Th2 immune responses.
The donating investigator reports that there is no difference in EGFP expression between mice with and without neo.
A targeting vector was designed to replace exon 2, including the initiation codon, of the programmed cell death 1 ligand 2 (Pdcd1lg2) gene with an enhanced green fluorescent protein (EGFP) and a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells, and chimeras were made by ES cell aggregation. The donating investigator reported that chimeric mice were bred to CMV-Cre mice on a BALB/c background - with the intention of removing the neo cassette (genotyping later determined it retained the neo cassette). The B7-DC KO colony was backcrossed at least nine generations to BALB/c mice (and the CMV-Cre was removed). Following this, the donating investigator reported they mice were backcrossed for at least 12 generations to C57BL/6NCrl (see SNP note below), and then sent to The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony. Genotyping performed at The Jackson Laboratory determined the B7-DC KO allele retained the neo cassette from the original construct.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While 1 of the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Allele Name | targeted mutation 2, Drew M Pardoll |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Pdcd1lg2, programmed cell death 1 ligand 2 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 19 |
Molecular Note | The signal peptide containing the ATG initiation codon was replaced with EGFP and a NEO cassette. This allele was subsequently used to generate |
Mutations Made By | Drew Pardoll, Johns Hopkins University School of Medic |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6N.129(Cg)-Pdcd1lg2tm2Dmp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017515 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Pdcd1lg2<tm1Dmp> |
Frozen Mouse Embryo | B6N.129(Cg)-Pdcd1lg2<tm2Dmp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129(Cg)-Pdcd1lg2<tm2Dmp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129(Cg)-Pdcd1lg2<tm2Dmp>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.129(Cg)-Pdcd1lg2<tm2Dmp>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.