| Allele Name | targeted mutation 1, Hans Clevers |
| Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
| Allele Synonym(s) | R26R-Brainbow2.1; R26R-Confetti |
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
| Gene Synonym(s) | |
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
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| Expressed Gene | GFP, Green Fluorescent Protein, |
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| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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| Expressed Gene | RFP, Red Fluorescent Protein, coral |
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| Site of Expression | Cre recombination results in stochastic multicolor fluorescent proteins being expressed from a single genomic locus in cre-expressing tissues. |
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| Strain of Origin | 129P2/OlaHsd |
| Chromosome | 6 |
| Molecular Note | A targeting vector containing (from 5' to 3') a strong CAGG promoter, a loxP site, a PGK-Neo-pA cassette (serving as a transcriptional roadblock), and the Brainbow 2.1 construct. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The Brainbow 2.1 construct was designed by Drs. Jeff Lichtman and Joshua Sanes (Harvard University) with four fluorescent protein sequences uniquely positioned in a tandem fashion and delimited by loxP sites in opposite orientation. Specifically, this Brainbow 2.1 coding region is composed of two adjacent floxed head-to-tail tandem dimers. The first head-to-tail dimer contains a loxP site and humanized Renilla GFP (hrGFPII; with nuclear localization signal plus polyA sequence) in forward orientation and a loxP site and monomeric EYFP (mYFPA206K plus polyA sequence) in reverse orientation. The second head-to-tail dimer contains a loxP site and tdimer2(12) RFP plus polyA sequence in forward orientation, and a loxP site and mCerulean CFP (with membrane tethering palmitoylation sequence plus polyA sequence) in reverse orientation. A single frt site is located at the 3' end of the Brainbow 2.1 construct. |
| Mutations Made By | Hans Clevers, Hubrecht Institute |
