B6(C)-Tg(UAS-EGFP,-SOD1*G37R)135Gsn/J

Stock number: 017458
Research areas: Neurobiology Research, Research Tools

Description

EGFP and the human SOD1 G37R mutation are driven by an inducible upstream activation sequence (UAS) promoter in this transgenic strain. These mice may be crossed with a GAL4 strain to activate gene expression in studies of familial amyotrophic lateral sclerosis (ALS) and motor neuron disease.

Detailed description

Enhanced green fluorescent protein (EGFP) and human SOD1 (superoxide dismutase 1, soluble) carrying the G37R mutation are each driven by the an inducible upstream activation sequence (UAS) promoter in this transgenic strain. Hemizygotes appear healthy, develop normally, and do not display any impairment of viability or reproductive capacity.

These animals express EGFP and SOD1 at very low levels. When crossed with a GAL4-expressing strain (e.g. Stock No. 017456 or Stock No. 017457 crossed with a Cre strain), EGFP and SOD1 are expressed at at higher levels. This strain may be effectively used as a component of the GAL4-UAS (Upstream Activation Sequence) system to activate gene transcription in studies of familial amyotrophic lateral sclerosis (ALS) and motor neuron disease.

Development

A construct carrying two 380 bp upstream activation sequence (UAS) DNA fragments that act as inducible promoters was designed to express enhanced green fluorescent protein (EGFP) and human SOD1 G37R mutant sequences (UAS-EGFP-UAS-SOD1(G37R)). The vector was introduced to C57BL/6 x BALB/c hybrid animals. This strain was backcrossed to C57BL/6 once by the donating laboratory.

Allele

Symbol: Tg(UAS-EGFP,-SOD1*G37R)135Gsn
Name: transgene insertion 135, Lawrence SB Goldstein
MGI accession ID: MGI:5287271
Generation method: Transgenic
Attributes: Reporter; Inducible; Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(UAS-EGFP,-SOD1*G37R)135Gsn
Marker name: transgene insertion 135, Lawrence SB Goldstein
Chromosome: UN
Strain of origin: C57BL/6 x BALB/c
Expressed genes: GFP,Green Fluorescent Protein,; SOD1,superoxide dismutase 1,human
Site of expression: GFP and mutant SOD1 expression is evident in neurons after GAL4-induction.
Molecular note: A construct carrying two 380 bp upstream activation sequence (UAS) DNA fragments that act as inducible promoters was designed to express an enhanced green fluorescent protein (EGFP) and human SOD1 G37R mutant sequences (UAS-EGFP-UAS-SOD1(G37R).