These OVE#2416B mice harbor a mutation created by random insertion of the Tyro-sd-IRES-loxP-FUGW lentiviral transgene (LV2187). Using inverse PCR analysis, the transgene integration site was identified in intron 5 of the WEE 1 homolog 1 gene (Wee1) on chromosome 7 (specifically at the 5'-117,273,626(+) bp position in antisense orientation). The donating investigator reports the phenotype of homozygous mice as: may be lethal at embryonic day (E)7.
Paul A Overbeek, Baylor College of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These OVE#2416B mice harbor a mutation created by random insertion of the Tyro-sd-IRES-loxP-FUGW lentiviral transgene (LV2187). Using inverse PCR analysis, the transgene integration site was identified in intron 5 of the WEE 1 homolog 1 gene (Wee1) on chromosome 7 (specifically at the 5'-117,273,626(+) bp position in antisense orientation). The donating investigator reports the phenotype of homozygous mice as: may be lethal at embryonic day (E)7.
A lentiviral transgenic approach was used to generate these mice. The Tyro-sd-IRES-loxP-FUGW lentiviral transgene (LV2187) was designed with a mouse tyrosinase minigene (Tyro) followed by a splice-donor::IRES::loxP sequence in the FUGW self-inactivating HIV-based lentiviral vector backbone. The Tyro-sd-IRES-loxP replaced the ubiquitin-c promoter, EGFP, and WPRE sequences originally found in the FUGW lentiviral vector. The Tyro minigene is composed of the mouse Tyr enhancer region (620 bp), promoter region (500 bp), and 1.7 kbp cDNA sequence (including the stop codon); all in antisense orientation relative to the FUGW backbone. The Tyro minigene is followed by an artificial splice donor sequence, an internal ribosome entry site (IRES), and a loxP site in this construct. This packaged lentiviral construct was injected subzonally (under the zona pellucida) into 1-8 cell stage FVB/N mouse embryos. Because of the random and multiple lentiviral integration sites of a transgene containing tyrosinase, founder mice (F0) may be identified by mosaic pigmentation. Founder mice from line OVE#2416B were bred with FVB/N mice, and pigmented offspring (F1) were bred to FVB/N mice. The resulting offspring (F2) with identical coat colors (light brown) were subsequently inbred. Using inverse PCR analysis, the transgene integration site was identified in intron 5 of the WEE 1 homolog 1 gene (Wee1) on chromosome 7 (specifically at the 5'-117,273,626(+) bp position in antisense orientation). Transgenic males were sent to The Jackson Laboratory Repository (MMRRC-JAX). Upon arrival, these mice were bred to FVB/NJ inbred mice for at least one generation to establish the live colony.
Expressed Gene | Tyr, tyrosinase, mouse, laboratory |
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Site of Expression |
Allele Name | transgene lentiviral insertion 2416B, Paul A Overbeek |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | OVE#2416B; OVE2416B |
Gene Symbol and Name | Wee1, WEE 1 homolog 1 (S. pombe) |
Gene Synonym(s) | |
Promoter | Tyr, tyrosinase, mouse, laboratory |
Expressed Gene | Tyr, tyrosinase, mouse, laboratory |
Strain of Origin | FVB/N |
Chromosome | 7 |
Molecular Note | The Tyro-IRES-sd-loxP-FUGW lentiviral transgene (LV2187) was designed with a mouse tyrosinase minigene (Tyro) followed by an IRES::3xATG::splice-donor::loxP sequence in the FUGW self-inactivating HIV-based lentiviral vector backbone. The Tyro-IRES-sd-loxP replaced the ubiquitin-c promoter, EGFP, and WPRE sequences originally found in the FUGW lentiviral vector. The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in antisense orientation relative to the FUGW backbone. The Tyro minigene is followed by an internal ribosome entry site (IRES) ending with ATGs in all three reading frames (3xATG), an artificial splice donor sequence, and a loxP site in this construct. In line 2416B, the lentiviral transgene inserted into intron 5 (NCBI37/mm9; 5'-117,273,626(+)) in antisense orientation relative to the disrupted gene. |
Mutations Made By | Paul Overbeek, Baylor College of Medicine |
When maintaining a live colony, heterozygous mice may be bred together, bred with wildtype siblings, or bred with FVB/N inbred mice.
When using the FVB/N-Wee1Tg(Tyr)2416BOve/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #36242 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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