B6;129S2-Cxadr^tm1.1Ics/J

Stock number: 017359
Research areas: Neurobiology Research, Research Tools

Description

These mice possess loxP sites flanking exon 2 of the Cxadr gene and have applications in studies of cardiac development.

Detailed description

These mice possess loxP sites on either side of exon 2 of the coxsackie virus and adenovirus receptor gene (Cxadr), a cell adhesion molecule critical in early embryogenesis. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.

When bred to a strain with widespread cre/Esr1 expression (Stock No. 00 4682), this mutant mouse strain may be useful in studies of cardiac, gastrointestinal, pancreatic, and thymic development and physiology.

Development

A targeting vector containing a FRT site flanked NEO cassette and loxP sites flanking exon 2 was utilized in the construction of this mutant. This construct was electroporated into 129S2/SvPas derived embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a FLP recombinase expression plasmid for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone FLP recombination and no longer retained the cassette but did retain the loxP-flanked exon 2 were injected in C57BL/6 blastocysts. Resulting chimeric animals were backcrossed to wildtype C57BL/6 mice. The mice were then backcrossed to C57BL/6 for 3 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Cxadr^tm1.1Ics
Name: targeted mutation 1.1, Mouse Clinical Institute
MGI accession ID: MGI:5086256
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Cxadr
Marker name: coxsackie virus and adenovirus receptor
Chromosome: 16
Strain of origin: 129S2/SvPas
Molecular note: Exon 2 was flanked with loxP sites and an FRT flanked neo cassette was inserted via homologous recombination. Flp mediated recombination removed the neo cassette. RT-PCR, western blot and indirect immunofluorescence confirmed normal mRNA and protein expression and localization in homozygous mice.