PV-Cre knock-in mice express Cre recombinase in parvalbumin-expressing neurons (such as interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia), without disrupting endogenous Pvalb expression. These mice may be useful in studying neuronal development, differentiation, and physiology.
Silvia Arber, Friedrich Miescher Institute
Genetic Background | Generation |
---|---|
N10F15
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing) | Pvalb | parvalbumin |
The PV-Cre knockin allele has the endogenous parvalbumin (Pvalb) promoter/enhancer elements directing Cre recombinase expression to Pvalb-expressing cells. Endogenous Pvalb expression remains intact. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in more than 90% of neurons that express parvalbumin, such as interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia. This mutant mouse strain represents a model that may be useful in studies of neuronal differentiation.
Of note: parvalbumin is expressed in sperm; use of male PV-Cre mice may result in unwanted germline recombination and global recombination.
If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Pvalbtm1(cre)Arbr). This same information would also be found searching the MGI Recombinase Activity database.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The Pvcre knock-in allele (also called Pvalb-IRES-Cre) was created in the laboratory of Dr. Silvia Arber (Friedrich Miescher Institute) to have a an IRES enhancer and cre sequence inserted immediately downstream of the parvalbumin locus (Pvalb) on chromosome 15. The specific details are below.
An IRES-Cre-pA targeting cassette containing an internal ribosome entry site (IRES), a Cre recombinase coding sequence, a TK-Neo-Hygro sequence and polyadenylation site sequence was inserted into the 3' UTR of exon 5 of the Pvalb locus. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygotes were intercrossed to produce homozygotes. In 2008, homozygous PV-Cre mice on a B6;129P2 genetic background were sent to The Jackson Laboratory Repository as Stock No. 008069. Upon arrival, some mice were subsequently backcrossed to C57BL/6J inbred mice (Stock No. 000664) for at least five generations to generate this C57BL/6J-congenic colony (Stock No. 017320).
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | most neurons that express parvalbumin including interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia |
Allele Name | targeted mutation 1, Silvia Arber |
---|---|
Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | (PV-CRE#32)SA-09; Parvalbumin-IRES-Cre; Parv-Cre; Prvlb-CRE; PvCre(Arbr); PVcre; PVIRES-Cre; PvalbCre; PVCre; PV-Cre; Pv-ires-Cre |
Gene Symbol and Name | Pvalb, parvalbumin |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | most neurons that express parvalbumin including interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 15 |
Molecular Note | An IRES-cre-pA cassette was inserted into the 3' UTR of exon 5. |
Mutations Made By | Silvia Arber, Friedrich Miescher Institute |
When maintaining a live colony, these mice can be bred as homozygotes.
Of note: parvalbumin is expressed in sperm; use of male PV-Cre mice may result in unwanted germline recombination and global recombination.
When using the B6 PVcre mouse strain in a publication, please cite the originating article(s) and include JAX stock #017320 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Pvalb<tm1(cre)Arbr> |
Frozen Mouse Embryo | B6.129P2-Pvalb<tm1(cre)Arbr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Pvalb<tm1(cre)Arbr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Pvalb<tm1(cre)Arbr>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Pvalb<tm1(cre)Arbr>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.