Overview

Also Known As: B6 PV^cre

PV-Cre knock-in mice express Cre recombinase in parvalbumin-expressing neurons (such as interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia), without disrupting endogenous Pvalb expression. These mice may be useful in studying neuronal differentiation.

Donating Investigator

Silvia Arber, Friedrich Miescher Institute

Genetic overview

Genetic Background	Generation
	N10F12 (2019-07-16 00:00:00)

Pvalb^tm1(cre)Arbr

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Pvalb	parvalbumin

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Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$255.00 Domestic price for female 4-week

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Details

Detailed Description

The PV-Cre knockin allele has the endogenous parvalbumin (Pvalb) promoter/enhancer elements directing Cre recombinase expression to Pvalb-expressing cells. Endogenous Pvalb expression remains intact. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in more than 90% of neurons that express parvalbumin, such as interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia. This mutant mouse strain represents a model that may be useful in studies of neuronal differentiation.
Of note: parvalbumin is expressed in sperm; use of male PV-Cre mice may result in unwanted germline recombination and global recombination.

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Pvalb^tm1(cre)Arbr). This same information would also be found searching the MGI Recombinase Activity database.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

An IRES-Cre-pA targeting cassette containing the Cre recombinase coding sequence, an internal ribosome entry site (IRES), and a polyadenylation sequence was inserted into the 3' UTR of exon 5 of the parvalbumin locus (Pvalb). The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygotes were intercrossed to produce homozygotes. In 2008, homozygous PV-Cre mice on a B6;129P2 genetic background were sent to The Jackson Laboratory Repository as Stock No. 008069. Upon arrival, some mice were subsequently backcrossed to C57BL/6J inbred mice (Stock No. 000664) for at least five generations to generate this C57BL/6J-congenic colony (Stock No. 017320).

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	most neurons that express parvalbumin including interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Genetics

Pvalb^tm1(cre)Arbr

Allele Symbol: Pvalb^tm1(cre)Arbr

Allele Name	targeted mutation 1, Silvia Arber
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	targeted mutation 1, Silvia Arber; Pvalb^tm1(cre)Arbr
Gene Symbol and Name	Pvalb, parvalbumin
Gene Synonym(s)	Pva; PV; Pva; Parv; D22S749; PALB1
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	most neurons that express parvalbumin including interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia
Strain of Origin	129P2/OlaHsd
Chromosome	15
Molecular Note	An IRES-cre-pA cassette was inserted into the 3' UTR of exon 5.
Mutations Made By	Silvia Arber, Friedrich Miescher Institute

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
- Neurobiology Research
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

References

Additional References

Antoine MW; Langberg T; Schnepel P; Feldman DE. 2019. Increased Excitation-Inhibition Ratio Stabilizes Synapse and Circuit Excitability in Four Autism Mouse Models. Neuron 101(4):648-661.e4PubMed: 30679017 MGI: J:271893

Additional - Pvalb^tm1(cre)Arbr related

Technical Support

Contact Technical Support

Genotyping Protocols
- Separated PCR: Pvalb^tm1(cre)Arbralternate2
- High Resolution Melting: Pvalb^tm1(cre)Arbralternate2
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
Of note: parvalbumin is expressed in sperm; use of male PV-Cre mice may result in unwanted germline recombination and global recombination.
- Additional Breeding and Husbandry Support
Citation
When using the B6 PV^cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #017320 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX11 (Maximum)

Pricing & Availability

Available Now

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Pvalb<tm1(cre)Arbr>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: B6 PVcre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Pvalbtm1(cre)Arbr

Allele Symbol: Pvalbtm1(cre)Arbr

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional References

Additional - Pvalbtm1(cre)Arbr related

Genotyping Protocols

Dietary Information

Breeding Considerations

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: B6 PV^cre

Pvalb^tm1(cre)Arbr

Allele Symbol: Pvalb^tm1(cre)Arbr

Additional - Pvalb^tm1(cre)Arbr related