These NOD background mice carry an autoreactive regulatory T cell receptor specific for insulin β chain peptide that renders them completely protected from diabetes development.
Li Wen, Yale School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
In addition to its biochemical functions, insulin can play an immunological role, acting as an autoantigen in human type I diabetes. In the NOD mouse model, many islet-reactive T cells invading the islet are insulin-specific. Studies suggest that insulin is also capable of generating a diabetes-protective immune response.
A regulatory/immunosuppressive T cell line (designated 2H6) was isolated from the pancreatic lymph nodes of a diabetic NOD mouse. These cells specifically recognize the insulin β chain peptide 12-25 or 9-23, secrete IFNG and TGFB, and have the ability to block both the adoptive transfer of diabetes in NOD-scid recipients and the spontaneous development of diabetes in NOD mice.
Based on this cell line, a transgenic strain that expresses the autoreactive 2H6 Vα6/Vβ14 regulatory T cell receptor was created on the NOD genetic background. Expression of the transgene blocks CD8+ T cell development. These mice are completely protected from diabetes development and upon binding express significant amounts of IFNG and TGFB. Cells from these mice can differentiate into potent regulatory cells that can protect NOD mice from the aggressive effects of spleen cells transferred from diabetic mice and diabetogenic cells from BDC2.5 transgenic mice.
No phenotypic differences between hemizygotes and homozygotes have been found.
This strain has been useful in studies of regulatory T cell (Treg) function and in the development of therapies for autoimmune disorders.
The donating laboratory isolated and cloned a regulatory/immunosuppressive T cell line (designated 2H6) from the pancreatic lymph nodes of a diabetic NOD mouse that specifically recognizes the insulin B chain peptide 12-25 or 9-23.
2H6 regulatory TCRα (Vα6 (Vα6/Jα45/Cα)) and TCRβ (Vβ14 (Vβ14/Dβ1.1Jβ2.3Cβ2)) constructs were directly coinjected into fertilized NOD oocytes to create a transgenic line. This strain was maintained on an NOD genetic background by the donating laboratory.
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
---|---|
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Site of Expression |
Allele Name | transgene insertion 2H6, Li Wen |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | 2H6 TCR transgenic |
Gene Symbol and Name | Tg(TcraTcrb)2H6Lwn, transgene insertion 2H6, Li Wen |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory |
Strain of Origin | NOD |
Chromosome | UN |
Molecular Note | The transgene contains the cloned regulatory/immunosuppressive T cell line (designated 2H6) from the pancreatic lymph nodes of a diabetic NOD mouse that specifically recognizes the insulin B chain peptide 12-25 or 9-23 (2H6 regulatory TCRalpha (Valpha6 (Valpha6/Jalpha45/Calpha)) and TCRbeta (Vbeta14 (Vbeta14/Dbeta1.1Jbeta2.3Cbeta2))). |
Homozygotes or hemizygotes may be bred to maintain a colony.
When using the NOD-Tg(TcraTcrb)2H6Lwn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017314 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or non carrier for Tg(TcraTcrb)2H6Lwn |
Frozen Mouse Embryo | NOD-Tg(TcraTcrb)2H6Lwn/J | $2595.00 |
Frozen Mouse Embryo | NOD-Tg(TcraTcrb)2H6Lwn/J | $2595.00 |
Frozen Mouse Embryo | NOD-Tg(TcraTcrb)2H6Lwn/J | $3373.50 |
Frozen Mouse Embryo | NOD-Tg(TcraTcrb)2H6Lwn/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.