These mice possess loxP sites flanking exons 3 and 4 of the high mobility group nucleosomal binding domain 3 ( Hmgn3) gene and have applications in studies related to regulation of insulin secretion.
Michael Bustin, NIH
These mice possess loxP sites on either side of exons 3 and 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3 and 4 deleted in the cre-expressing tissue(s).
A FRT site flanked targeting vector containing PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette and aloxP site was inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 3. This construct was electroporated into (C57BL/6J x 129S4/SvJae)F1 derived v6.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to transgenic mice (on a mixed B6;SJL genetic background) expressing Flp recombinase. The donating investigator reported that mice that retained the loxP site flanked exons 3 and 4 were then bred to C57BL/6N mice (see SNP note below) for 10 generations and to remove the Flp transgene. Heterozygotes were crossed to generate homozygotes.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6NJ (Stock No. 005304) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1.1, Michael Bustin|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Hmgn3, high mobility group nucleosomal binding domain 3|
|Strain of Origin||(C57BL/6J x 129S4/SvJae)F1|
|Molecular Note||A loxP site was inserted upstream of exon 3, and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 4. Germ line, flp mediated recombination removed the neo cassette leaving exons 3 and 4 floxed.|
|Mutations Made By|| |
Michael Bustin, NIH
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6N.Cg-Hmgn3tm1.1Mbus/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017297 in your Materials and Methods section.