B6.129X1-Hmgn1^tm1Mbus/J

Stock number: 017296
Research areas: Cancer Research, Cell Biology Research, Neurobiology Research

Description

Embryonic fibroblasts from these Hmgn1, high mobility group nucleosomal binding domain 1, knock-out mice exhibit defective DNA repair. Homozygous Hmgn1 knock-out mice exhibit decreased exploratory behavior and preference social novelty.

Detailed description

Mice that are homozygous for the targeted mutation are viable and fertile. Certain antibodies to HMGN1 detect a short version of HMGN1 protein by Western blot analysis when large amounts of MEFs from homozygous animals are analyzed. Mice homozygous for this targeted mutation, and on a congenic C57BL/6 background, do not exhibit the perinatal lethality seen in homozygotes on the 129 and mixed B6;129 backgrounds. Homozygotes exhibit increased histone acetylation in brain tissue, and reduced levels of exploratory behavior and preference for social novelty.

MEFs isolated from homozygous mice on the congenic C57BL/6 background exhibit defective DNA repair. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector containing a NEO cassette was used to disrupt exons 2, 3 and part of exon 4. The construct was electroporated into 129X1/SvJ derived ESVJ-1183 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6J for 10 generations, using a speed congenic protocol. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Hmgn1^tm1Mbus
Name: targeted mutation 1, Michael Bustin
MGI accession ID: MGI:3590648
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Hmgn1
Marker name: high mobility group nucleosomal binding domain 1
Chromosome: 16
Strain of origin: 129X1/SvJ
Molecular note: Exons 2, 3 and part of 4 were replaced with a neomycin resistance cassette. Western blot of mutant MEFs confirmed absence of protein. Heterozygotes expressed approximately half of that detected in wild-type cells.