B6.129X1(Cg)-Pkd2^tm1.1Tjwt/J

Stock number: 017292
Research areas: Cell Biology Research, Developmental Biology Research, Endocrine Deficiency Research, Internal/Organ Research, Research Tools

Description

These Pkd2^cond mutant mice possess loxP sites flanking exons 11-13 of the polycystic kidney disease 2 (Pkd2) gene. This strain may be useful for studying renal development in autosomal dominant polycystic kidney disease.

Detailed description

These Pkd2^cond mutant mice possess loxP sites flanking exons 11-13 of the polycystic kidney disease 2 (Pkd2) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. PKD2 is a calcium-permeable membrane channel expressed in fetal kidneys and in most adult tissues. Along with PKD1, PKD2 regulates cell proliferation, cell migration, and interactions with other cells, and is necessary for normal development and function of the kidneys. Mutations in PKD2 are responsible for 15% of all cases of autosomal dominant polycystic kidney disease (ADPKD). When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 11-13 deleted in the cre-expressing tissues. This strain may be useful for studying renal development in ADPKD.

For example, when crossed to a strain expressing Cre recombinase in endothelial cells (see Stock No. 008863), this mutant mouse strain may be useful in studies of placental development.

Development

A targeting vector was designed to insert a loxP site upstream of exon 11, and a second loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, downstream of exon 13 of the polycystic kidney disease 2 (Pkd2) gene. The construct was electroporated into 129X1/SvJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6J mice. Offspring were bred with Flp transgenic mice on a C57BL/6 background to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene, resulting in a colony homozygous for the floxed-Pkd2 (Pkd2^cond) allele. These mice were then backcrossed at least 10 generations to C57BL/6J mice. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Pkd2^tm1.1Tjwt
Name: targeted mutation 1.1, Terry J Watnick
MGI accession ID: MGI:4843126
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Pkd2
Marker name: polycystic kidney disease 2
Chromosome: 5
Strain of origin: 129X1/SvJ
Molecular note: A loxP site was inserted upstream of exon 11 and an FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 13. Flp-mediated recombination removed the neo cassette leaving exons 11 through 13 floxed.