JAX Mouse Strain Datasheet - 016999

B6;129S6-Gt(ROSA)26Sor^{tm1(rtTA2S*M2)Whsu}/J

Stock No:: 016999

Cryo Recovery

Overview

This R26STOPrtTA mutant mouse strain combines two popular conditionally-regulated expression methodologies (Tet Expression and Cre Recombinase) providing a tool for doxycycline-inducible spatiotemporal and lineage specific expression of genes.

Donating Investigator

Frank Costantini, Columbia University Medical Center

Genetic overview

Genetic Background	Generation

Gt(ROSA)26Sor^{tm1(rtTA2SM2)Whsu}*

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), Transactivator)	Gt(ROSA)26Sor	gene trap ROSA 26, Philippe Soriano

View Genetics

Research Applications

Neurobiology Research
Research Tools

View All Research Applications

Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

These mice contain the rtTAS*M2, or rtTA2^S-M2, an optimized form of reverse tetracycline regulated transactivator, gene inserted into the Gt(ROSA)26Sor locus. Expression of the rtTAS*M2 is blocked by a loxP-flanked STOP fragment placed between the rtTAS*M2 sequence and the Gt(ROSA)26Sor promoter. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will express rtTAS*M2, reverse tetracycline regulated transactivator, in the cre expressing cells. When bred to mice that also carry a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

Development

A targeting vector was used to insert a loxP flanked STOP sequence followed by an optimized reverse tetracycline-controlled transactivator, rtTAS*M2, or rtTA2^S-M2, into Gt(ROSA)26Sor.The construct was electroporated into 129S6/SvEvTac derived CSL3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice for an unknown number of generations. Homozygote males were then crossed to wildtype 129S6/SvEvTac females. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Suggestions

Wild-type from the colony

Approximate Controls

101043 B6129SF1/J

Additional Information

Considerations for Choosing Controls

Selected References

Yu HM; Liu B; Chiu SY; Costantini F; Hsu W. 2005. Development of a unique system for spatiotemporal and lineage-specific gene expression in mice. Proc Natl Acad Sci U S A 102(24):8615-20. PubMed: 15941831 MGI: J:99718

View All References

Genetics

Gt(ROSA)26Sor^{tm1(rtTA2SM2)Whsu}*

Allele Symbol: Gt(ROSA)26Sor^{tm1(rtTA2SM2)Whsu}*

Allele Name	targeted mutation 1, Wei Hsu
Allele Type	Targeted (Conditional ready (e.g. floxed), Transactivator)
Allele Synonym(s)	Gt(ROSA)26Sor^{tm1(xstpx-rtTA2S*M2)Whsu}; R26STOPrtTA
Gene Symbol and Name	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Gene Synonym(s)	AV258896; Gtrgeo26; Gtrgeo26; Gtrosa26; Gtrosa26; R26; ROSA26; SETD5-AS1; Thumpd3as1; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26
Site of Expression	When mice carrying this allele are bred to mice that express Cre recombinase, the resulting offspring will express rtTAS*M2, reverse tetracycline regulated transactivator, in the cre expressing cells. When bred to mice that also carry a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed.
Strain of Origin	129S6/SvEvTac
Chromosome	6
Molecular Note	A synthetic modified version of the rtTA gene preceded by a loxP flanked STOP sequence was inserted into the Gt(ROSA)26Sor locus. This version of rtTA contains 5 amino acid changes in the TetR moiety (S12G, E19G, A56P, D148E, and H179R) and a synthetic optimized transactivating domain. This rtTA has reduced basal activity and enhanced doxycycline sensitivity compared to wild-type rtTA.
Mutations Made By	Frank Costantini, Columbia University Medical Center

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Cre-lox System
  - loxP-flanked Sequences
- Tet Expression System
  - tTA/rtTA Expressing Strains
Research Tools
- Cre-lox System
  - loxP-flanked Sequences
- Developmental Biology Research
  - Cre-lox System
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: Cre-lox System
- Reproductive Biology Research
  - Cre-lox System
- Tet Expression Systems
  - tTA/rtTA Expressing Strains

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Gt(ROSA)26Sor^{tm1(rtTA2SM2)Whsu}/Gt(ROSA)26Sor^{tm1(rtTA2SM2)Whsu}
involves: 129S6/SvEvTac

normal phenotype

no abnormal phenotype detected
- no overt phenotype is detected

References

Yu HM; Liu B; Chiu SY; Costantini F; Hsu W. 2005. Development of a unique system for spatiotemporal and lineage-specific gene expression in mice. Proc Natl Acad Sci U S A 102(24):8615-20. PubMed: 15941831 MGI: J:99718

Additional - Gt(ROSA)26Sor^{tm1(rtTA2SM2)Whsu}* related

Hsu W; Mirando AJ; Yu HM. 2009. Manipulating gene activity in Wnt1-expressing precursors of neural epithelial and neural crest cells. Dev Dyn 239(1):338-345. PubMed: 19653308 MGI: J:155252

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Gt(ROSA)26Sor<tm1(xstpx-rtTA2S*M2)Whsu>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Gt(ROSA)26Sortm1(rtTA2S*M2)Whsu

Allele Symbol: Gt(ROSA)26Sortm1(rtTA2S*M2)Whsu

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gt(ROSA)26Sortm1(rtTA2S*M2)Whsu/Gt(ROSA)26Sortm1(rtTA2S*M2)Whsuinvolves: 129S6/SvEvTac

normal phenotype

References

Additional - Gt(ROSA)26Sortm1(rtTA2S*M2)Whsu related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Gt(ROSA)26Sor^{tm1(rtTA2SM2)Whsu}*

Allele Symbol: Gt(ROSA)26Sor^{tm1(rtTA2SM2)Whsu}*

Genotype: Gt(ROSA)26Sor^{tm1(rtTA2SM2)Whsu}/Gt(ROSA)26Sor^{tm1(rtTA2SM2)Whsu}
involves: 129S6/SvEvTac

Additional - Gt(ROSA)26Sor^{tm1(rtTA2SM2)Whsu}* related