These transgenic mice express myc-tagged mouse Axin1 and EGFP under the control of tetO, tetracycline operator (tetracycline-responsive element, TRE). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue overexpression of Axin1 and expression of EGFP may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring.

Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
This strain may be useful in generating bi-transgenic mutant mice for the reversible, inducible overexpression of Axin1 and the inhibition of Wnt signaling.

These TRE2-Axin-GFP transgenic mice carry myc-tagged mouse Axin1 and EGFP regulated by a tetracycline operator (tetO). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue Axin1 and EGFP expression may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

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