These TRE2-Axin-GFP transgenic mice carry myc-tagged mouse Axin1 and EGFP regulated by a tetracycline operator (tetO). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue Axin1 and EGFP expression may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring.
Frank Costantini, Columbia University Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter, Inducible, Inserted expressed sequence) |
These transgenic mice express myc-tagged mouse Axin1 and EGFP under the control of tetO, tetracycline operator (tetracycline-responsive element, TRE). When mated to a mutant strain expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue overexpression of Axin1 and expression of EGFP may be regulated with the tetracycline analog doxycycline (dox) in the double mutant offspring.
Mice that are hemizygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make this strain homozygous.
This strain may be useful in generating bi-transgenic mutant mice for the reversible, inducible overexpression of Axin1 and the inhibition of Wnt signaling.
A transgenic construct containing myc-tagged mouse Axin1 coding sequence, IRES2, and eGFP sequence, under the control of tetO, tetracycline operator (tetracycline-responsive element, TRE), was injected into fertilized B6CBAF2 mouse eggs. Founder line TA6 was subsequently established. The mice were then backcrossed to C57BL/6J for 5 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | Axin1, axin 1, mouse, laboratory |
Site of Expression | When bred to mice expressing a tetracycline transactivator (tTA) or reverse transactivator (rtTA), doxycycline induction results in the expression of Axin1 and GFP in transactivator expressing tissues of the offspring. |
Allele Name | transgene insertion TA6, Frank Costantini |
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Allele Type | Transgenic (Reporter, Inducible, Inserted expressed sequence) |
Allele Synonym(s) | TRE2-Axin-GFP |
Gene Symbol and Name | Tg(TetO-Axin1,EGFP)TA6Cos, transgene insertion TA6, Frank Costantini |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | Axin1, axin 1, mouse, laboratory |
Site of Expression | When bred to mice expressing a tetracycline transactivator (tTA) or reverse transactivator (rtTA), doxycycline induction results in the expression of Axin1 and GFP in transactivator expressing tissues of the offspring. |
Strain of Origin | (C57BL/6 x CBA)F2 |
Chromosome | UN |
Molecular Note | The transgene construct contains a myc-tagged mouse Axin1 coding sequence, IRES2, and eGFP sequence, under the control of tetO, tetracycline operator (tetracycline-responsive element, TRE). Line TA6 was established. |
Mutations Made By | Frank Costantini, Columbia University Medical Center |
When maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator has not attempted to make this strain homozygous.
When using the B6.Cg-Tg(TetO-Axin1,EGFP)TA6Cos/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016998 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(TetO-Axin1,EGFP)TA6Cos |
Frozen Mouse Embryo | B6.Cg-Tg(TetO-Axin1 EGFP)TA6Cos/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(TetO-Axin1 EGFP)TA6Cos/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(TetO-Axin1 EGFP)TA6Cos/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(TetO-Axin1 EGFP)TA6Cos/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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