Vglut2-ires-Cre knock-in mice have Cre recombinase expression directed to excitatory glutamatergic neuron cell bodies, without disrupting endogenous vesicular glutamate transporter 2 expression. These mice may be used to generate conditional mutations for studying gain-or-loss of function and/or fate mapping related to glutamatergic neurons.
Of note, the same Vglut2-ires-Cre knock-in allele is also available on a C57BL/6J genetic background as Stock No. 028863.
Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)
Genetic Background | Generation |
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?+pN1F10
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Slc17a6 | solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 |
Mice that are homozygous for the Slc17a6tm2(cre)Lowl (also called Vglut2-ires-Cre) knock-in mutation are viable and fertile with no reported abnormalities. Cre recombinase activity is detected in excitatory glutamatergic, VGLUT2 positive, neuron cell bodies in the thalamus, paraventricular nucleus, nucleus of the lateral olfactory tract, basolateral nucleus of the amygdala, ventromedial hypothalamus, piriform cortex, posterior hypothalamus, ventral premammillary nucleus, subthalamic nucleus, medial geniculate nucleus, reticulotegmental nucleus, pontine gray, external cuneate nucleus, and lateral reticular nucleus. When crossed with a strain containing loxP sequences, Cre-mediated recombination results in tissue-specific deletion of flanked sequences in the offspring.
The Vglut2-ires-Cre knock-in allele was designed by Dr. Bradford Lowell (Beth Israel Deaconess Medical Center / Harvard). A targeting vector was designed to insert an internal ribosome entry site-linked Cre recombinase gene downstream of the stop codon of the solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 gene (Slc17a6 or VGLUT2) on chromosome 7. The construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then crossed to FVB. In 2012, Vglut2-ires-Cre knock-in mice on a mixed C57BL/6;FVB;129S6 genetic background (with black coat color) were sent to The Jackson Laboratory Repository. Upon arrival, sperm was cryopreserved. An aliquot of that frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664) and rederive our live colony as Stock No. 016963, which was thereafter maintained by breeding mutant mice together.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase activity is detected in excitatory glutamatergic, VGLUT2 positive, neuron cell bodies in the thalamus, paraventricular nucleus, nucleus of the lateral olfactory tract, basolateral nucleus of the amygdala, ventromedial hypothalamus, piriform cortex, posterior hypothalamus, ventral premammillary nucleus, subthalamic nucleus, medial geniculate nucleus, reticulotegmental nucleus, pontine gray, external cuneate nucleus, and lateral reticular nucleus. |
Allele Name | targeted mutation 2, Bradford B Lowell |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | VGAT-Cre; Vglut2Cre; Vglut2-ires-Cre |
Gene Symbol and Name | Slc17a6, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase activity is detected in excitatory glutamatergic, VGLUT2 positive, neuron cell bodies in the thalamus, paraventricular nucleus, nucleus of the lateral olfactory tract, basolateral nucleus of the amygdala, ventromedial hypothalamus, piriform cortex, posterior hypothalamus, ventral premammillary nucleus, subthalamic nucleus, medial geniculate nucleus, reticulotegmental nucleus, pontine gray, external cuneate nucleus, and lateral reticular nucleus. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 7 |
Molecular Note | A targeting vector, constructed from a BAC clone containing the Slc17a6 (Vglut2) gene, was utilized to insert an internal ribosomal entry site followed by the cre recombinase sequence into the Slc17a6 locus just downstream of the endogenous stop codon. Cre is expressed from the Slc17a6 gene promoter. |
Mutations Made By | Dr. Jia Yu, Beth Israel Deaconess Medical Center |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Vglut2-ires-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #016963 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Slc17a6<tm2(cre)Lowl> |
Frozen Mouse Embryo | STOCK Slc17a6<tm2(cre)Lowl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Slc17a6<tm2(cre)Lowl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Slc17a6<tm2(cre)Lowl>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Slc17a6<tm2(cre)Lowl>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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