Overview

Also Known As: Vglut2-ires-cre

Vglut2-ires-Cre knock-in mice have Cre recombinase expression directed to excitatory glutamatergic neuron cell bodies, without disrupting endogenous vesicular glutamate transporter 2 expression. These mice may be used to generate conditional mutations for studying gain-or-loss of function and/or fate mapping related to glutamatergic neurons.
Of note, the same Vglut2-ires-Cre knock-in allele is also available on a C57BL/6J genetic background as Stock No. 028863.

Donating Investigator

Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)

Genetic overview

Genetic Background	Generation
	?+pN1F10 (2019-11-01 00:00:00)

Slc17a6^tm2(cre)Lowl

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Slc17a6	solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6

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Research Applications

Research Tools
Cancer Research
Neurobiology Research

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Base Price

Starting at:

$333.00 Domestic price for female 4-week

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Details

Detailed Description

Mice that are homozygous for the Slc17a6^tm2(cre)Lowl (also called Vglut2-ires-Cre) knock-in mutation are viable and fertile with no reported abnormalities. Cre recombinase activity is detected in excitatory glutamatergic, VGLUT2 positive, neuron cell bodies in the thalamus, paraventricular nucleus, nucleus of the lateral olfactory tract, basolateral nucleus of the amygdala, ventromedial hypothalamus, piriform cortex, posterior hypothalamus, ventral premammillary nucleus, subthalamic nucleus, medial geniculate nucleus, reticulotegmental nucleus, pontine gray, external cuneate nucleus, and lateral reticular nucleus. When crossed with a strain containing loxP sequences, Cre-mediated recombination results in tissue-specific deletion of flanked sequences in the offspring.

Development

The Vglut2-ires-Cre knock-in allele was designed by Dr. Bradford Lowell (Beth Israel Deaconess Medical Center / Harvard). A targeting vector was designed to insert an internal ribosome entry site-linked Cre recombinase gene downstream of the stop codon of the solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 gene (Slc17a6 or VGLUT2) on chromosome 7. The construct was electroporated into 129S6/SvEvTac derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then crossed to FVB. In 2012, Vglut2-ires-Cre knock-in mice on a mixed C57BL/6;FVB;129S6 genetic background (with black coat color) were sent to The Jackson Laboratory Repository. Upon arrival, sperm was cryopreserved. An aliquot of that frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664) and rederive our live colony as Stock No. 016963, which was thereafter maintained by breeding mutant mice together.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is detected in excitatory glutamatergic, VGLUT2 positive, neuron cell bodies in the thalamus, paraventricular nucleus, nucleus of the lateral olfactory tract, basolateral nucleus of the amygdala, ventromedial hypothalamus, piriform cortex, posterior hypothalamus, ventral premammillary nucleus, subthalamic nucleus, medial geniculate nucleus, reticulotegmental nucleus, pontine gray, external cuneate nucleus, and lateral reticular nucleus.

Expressed Gene

cre, cre recombinase, bacteriophage P1

Site of Expression

Cre recombinase activity is detected in excitatory glutamatergic, VGLUT2 positive, neuron cell bodies in the thalamus, paraventricular nucleus, nucleus of the lateral olfactory tract, basolateral nucleus of the amygdala, ventromedial hypothalamus, piriform cortex, posterior hypothalamus, ventral premammillary nucleus, subthalamic nucleus, medial geniculate nucleus, reticulotegmental nucleus, pontine gray, external cuneate nucleus, and lateral reticular nucleus.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Vong L; Ye C; Yang Z; Choi B; Chua S Jr; Lowell BB. 2011. Leptin Action on GABAergic Neurons Prevents Obesity and Reduces Inhibitory Tone to POMC Neurons. Neuron 71(1):142-54PubMed: 21745644 MGI: J:174691

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Genetics

Slc17a6^tm2(cre)Lowl

Allele Symbol: Slc17a6^tm2(cre)Lowl

Allele Name	targeted mutation 2, Bradford B Lowell
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Slc17a6^tm2(cre)Lowl; targeted mutation 2, Bradford B Lowell
Gene Symbol and Name	Slc17a6, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Gene Synonym(s)	DNPI; 2900073D12Rik; 2900073D12Rik; VGLUT2; RIKEN cDNA 2900073D12 gene; Vglut2; Dnpi
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is detected in excitatory glutamatergic, VGLUT2 positive, neuron cell bodies in the thalamus, paraventricular nucleus, nucleus of the lateral olfactory tract, basolateral nucleus of the amygdala, ventromedial hypothalamus, piriform cortex, posterior hypothalamus, ventral premammillary nucleus, subthalamic nucleus, medial geniculate nucleus, reticulotegmental nucleus, pontine gray, external cuneate nucleus, and lateral reticular nucleus.
Strain of Origin	129S6/SvEvTac
Chromosome	7
Molecular Note	A targeting vector, constructed from a BAC clone containing the Slc17a6 (Vglut2) gene, was utilized to insert an internal ribosomal entry site followed by the cre recombinase sequence into the Slc17a6 locus just downstream of the endogenous stop codon. Cre is expressed from the Slc17a6 gene promoter.
Mutations Made By	Dr. Jia Yu, Beth Israel Deaconess Medical Center

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Neurobiology Research
  - cell marker
- Genetics Research
  - Tissue/Cell Markers: neurons
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: Cre-lox System
- Developmental Biology Research
  - Cre-lox System
- Cancer Research
  - Cre-lox System
- Cardiovascular Research
  - Cre-lox System
- Cre-lox System
  - Cre Recombinase Expression
Cancer Research
- Cre-lox System
  - Cre recombinase expression
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Slc17a6^tm2(cre)Lowl/Slc17a6⁺
involves: 129S6/SvEvTac

normal phenotype

no abnormal phenotype detected
- Normal - heterozygotes do not display a detectable abnormal phenotype

References

Vong L; Ye C; Yang Z; Choi B; Chua S Jr; Lowell BB. 2011. Leptin Action on GABAergic Neurons Prevents Obesity and Reduces Inhibitory Tone to POMC Neurons. Neuron 71(1):142-54PubMed: 21745644 MGI: J:174691

Additional References

Fasano C; Rocchetti J; Pietrajtis K; Zander JF; Manseau F; Sakae DY; Marcus-Sells M; Ramet L; Morel LJ; Carrel D; Dumas S; Bolte S; Bernard V; Vigneault E; Goutagny R; Ahnert-Hilger G; Giros B; Daumas S; Williams S; El Mestikawy S. 2017. Regulation of the Hippocampal Network by VGLUT3-Positive CCK- GABAergic Basket Cells. Front Cell Neurosci 11:140PubMed: 28559797 MGI: J:274480
Fu O; Iwai Y; Narukawa M; Ishikawa AW; Ishii KK; Murata K; Yoshimura Y; Touhara K; Misaka T; Minokoshi Y; Nakajima KI. 2019. Hypothalamic neuronal circuits regulating hunger-induced taste modification. Nat Commun 10(1):4560PubMed: 31594935 MGI: J:281368

Additional - Slc17a6^tm2(cre)Lowl related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Slc17a6^tm2(cre)Lowl
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the Vglut2-ires-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #016963 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX12 (Maximum)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Slc17a6<tm2(cre)Lowl>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	STOCK Slc17a6<tm2(cre)Lowl>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	STOCK Slc17a6<tm2(cre)Lowl>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	STOCK Slc17a6<tm2(cre)Lowl>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	STOCK Slc17a6<tm2(cre)Lowl>/J Frozen Embryo	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: Vglut2-ires-cre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Slc17a6tm2(cre)Lowl

Allele Symbol: Slc17a6tm2(cre)Lowl

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Slc17a6tm2(cre)Lowl/Slc17a6+involves: 129S6/SvEvTac

normal phenotype

References

Additional References

Additional - Slc17a6tm2(cre)Lowl related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Slc17a6^tm2(cre)Lowl

Allele Symbol: Slc17a6^tm2(cre)Lowl

Genotype: Slc17a6^tm2(cre)Lowl/Slc17a6⁺
involves: 129S6/SvEvTac

Additional - Slc17a6^tm2(cre)Lowl related