Lang-EGFP mutant mice express enhanced green fluorescent protein downstream of the internal stop codon of the CD207 antigen gene. These mice may be useful for visualizing Langerhans cells and monitoring their movement during inflammation.
Bernard Malissen, Centre d'immunologie de Marseille Luminy
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Cd207 | CD207 antigen |
Lang-EGFP mutant mice contain an internal ribosome entry site (IRES) and an enhanced green fluorescent protein (EGFP) downstream of the internal stop codon of the CD207 antigen (Cd207 or Langerin) gene. Homozygotes are viable, fertile, and normal in size. Langerin is a transmembrane receptor produced by specialized antigen-presenting cells located in the skin called Langerhans cells (LCs), and other dendritic cells including dermal DCs and splenic CD8+ DCs. Langerin is specifically localized in the Birbeck granules, where it is necessary for their formation. In these mice, EGFP expression is evident in the cytosol of immature Langerin-derived DCs and CD8+ DCs and is decreased upon LC maturation. These mice may be useful for visualizing Langerhans cells and monitoring their movement during inflammation.
The Lang-EGFP targeting vector was designed to contain an internal ribosome entry site (IRES), and an enhanced green fluorescent protein (EGFP), followed by a self-excising loxP-flanked ACN cassette (itself containing a neomycin resistance (Neo) gene and a testes-specific angiotensin-converting enzyme promoter-driven Cre recombinase gene), which self-excises during transmission in the male chimera. This construct was inserted downstream of the internal stop codon of the CD207 antigen (Cd207 or Langerin) gene. This construct was injected into 129S2/SvPas-derived CK35 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts, and the resulting chimeric males were crossed to C57BL/6 females. The donating investigator reported the mice were backcrossed to C57BL/6 mice for at least 15 generations (see SNP results below) prior to sending males to The Jackson Laboratory Repository in 2012. Upon arrival, males were used to cryopreserve sperm. To establish the living mouse colony, an aliquot of the frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
In 2013, a 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data show that the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background, and the living colony in 2103 is on a mixed C57BL/6N;C57BL/6J genetic background.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP expression is evident in the cytosol of immature Langerin-derived DCs and CD8+ DCs and is decreased upon LC maturation. |
Allele Name | targeted mutation 1, Bernard Malissen |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | Lang-EGFP; muLangerin-EGFP |
Gene Symbol and Name | Cd207, CD207 antigen |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP expression is evident in the cytosol of immature Langerin-derived DCs and CD8+ DCs and is decreased upon LC maturation. |
Strain of Origin | 129S2/SvPas |
Chromosome | 6 |
Molecular Note | A vector containing an IRES-EGFP was inserted in the 3' UTR with a Cre-neo auto-deleter cassette, which directs its own excision as it passes through the male germ line. |
Mutations Made By | Bernard Malissen, Centre d'immunologie de Marseille Luminy |
When maintaining a live colony, homozygous mice may be bred together.
When using the Lang-EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #016939 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Cd207<tm2Mal> |
Frozen Mouse Embryo | B6.129S2-Cd207<tm2Mal>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Cd207<tm2Mal>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Cd207<tm2Mal>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S2-Cd207<tm2Mal>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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