These mice carry a floxed allele of the Cd1d1 (CD1d1 antigen) gene. This strain may be useful in studies of natural killer T (NKT) cells.
Albert Bendelac , University of Chicago
These mice carry a floxed allele of the Cd1d1 (CD1d1 antigen) gene. When bred to mice expressing Cre recombinase under the control of a chosen promoter, exons 2-6 are deleted in the tissue of interest, blocking expression of Cd1d1. As the homologous Cd1d2 gene has a frameshift mutation preventing expression of a functional protein, blocking expression of Cd1d1 results in the complete absence of CD1D proteins at the cell surface. Homozygous floxed mice are viable and fertile and do not display any gross physical or behavioral abnormalities.
When crossed with Cd4-Cre mice (see Stock No. 017336), progeny reportedly show an 8-fold reduction in natural killer T (NKT) cell numbers on average in a manner commensurate with the levels of CD1D protein downregulation achieved among cortical thymocytes. This result is consistent with the requirement for Cd1d1 expression by CD4+ CD8+ double positive (DP) thymocytes for NKT cell development.
When these floxed mice are crossed with Itfax (Cd11c) (see Stock No. 007567), Lyz2 (see Stock No. 004781), and Cd19 (see Stock No. 006785) -Cre mice, which lack CD1D on dendritic cells (DCs), macrophages and B cells, respectively, progeny exhibit normal development and distribution of NKT cells. The Cd11c-Cre cross progeny lose expression in all DCs and in some macrophage subsets that express intermediate levels of CD11C. A small fraction of B cells also lose CD1D in these mice. Mice selectively lacking CD1D from one of the antigen-presenting cell types have normoresponsive NKT cells that produce similar amounts of IL4 and IFNγ as their wildtype littermates.
Exons 2-6 and an FRT-flanked neomycin cassette were flanked by loxP sites in an embryonic stem (ES) cell line of C57BL/6 origin. The neomycin selection cassette was excised from the resulting mice through crosses with a C57BL/6 background FLP strain. The donating laboratory used C57BL/6J mice to maintain their colony.
|Allele Name||targeted mutation 1.1, Albert Bendelac|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||CD1.1 Conditional; CD1dfl; Cd1d1/Cd1d2tm1.1Aben; cd1d1fl|
|Gene Symbol and Name||Cd1d1, CD1d1 antigen|
|Strain of Origin||C57BL/6|
|General Note||Because the C57BL/6 ES cells used to generate the allele contain a natural frameshift mutation in Cd1d2 that prevents its surface expression, deletion of Cd1d1 is sufficient to abolish all CD1D protein from the cell surface.|
|Molecular Note||Exons 2-6 were flanked by loxP sites. Flp-mediated recombination removed the neo cassette.|
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the C57BL/6-Cd1d1tm1.1Aben/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016929 in your Materials and Methods section.