129;B6-Dpysl5^tm1Gosh/J

Stock number: 016928
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools

Description

In this strain an IRES-lacZ-neo cassette replaces exons 2-6 of the Dpysl4 gene. These mice exhibit abnormally small Purkinje cells in the cerebellum during the period of dendritic branching and display long-term depression of excitatory synaptic transmissions.

Detailed description

In this strain an IRES-lacZ-neo cassette replaces exons 2-6 of the dihydropyrimidinase-like 5 (Dpysl4 or Crmp5) gene. Homozygotes are viable and fertile. CRMP-5 is a member of a family of phosphoproteins that are involved in dendritic length and branching, semaphorin signaling pathway, growth cone collapse, and neurite extension after binding with tubulin heterodimers. It is expressed throughout the nervous system after P14 and is specifically involved in filopodia and growth cone morphology in neurons. These mice exhibit abnormally small Purkinje cells in the cerebellum during the period of dendritic branching. They also display long-term depression of excitatory synaptic transmissions. This strain may be useful for studying nervous system developmental disorders.

Development

A targeting vector was designed to replace exons 2-6 of the dihydropyrimidinase-like 5 (Dpysl4 or Crmp5) gene with an internal ribosome entry site (IRES)-β-galactosidase (lacZ) followed by a neomycin resistance cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred with C57BL/6 females for germline transmission. A chimeric male offspring was then bred to 129/SvE females. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation.

Allele

Symbol: Dpysl5^tm1Gosh
Name: targeted mutation 1, Yoshio Goshima
MGI accession ID: MGI:4941238
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Dpysl5
Marker name: dihydropyrimidinase-like 5
Marker synonyms: CRAM; CRMP5; CRMP-5; CV2; RTSC4; Ulip6
Chromosome: 5
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: Dpysl5 is normally expressed throughout the nervous system after P14 and is specifically involved in filopodia and growth cone morphology in neurons.
Molecular note: Exons 2 through 6 were replaced with an IRES/lacZ/neo cassette. The absence of protein expression was confirmed by western blot analysis on brain extracts.