In this strain an IRES-lacZ-neo cassette replaces exons 2-6 of the Dpysl4 gene. These mice exhibit abnormally small Purkinje cells in the cerebellum during the period of dendritic branching and display long-term depression of excitatory synaptic transmissions.
Pappachan Kolattukudy, University of Central Florida
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Dpysl5 | dihydropyrimidinase-like 5 |
In this strain an IRES-lacZ-neo cassette replaces exons 2-6 of the dihydropyrimidinase-like 5 (Dpysl4 or Crmp5) gene. Homozygotes are viable and fertile. CRMP-5 is a member of a family of phosphoproteins that are involved in dendritic length and branching, semaphorin signaling pathway, growth cone collapse, and neurite extension after binding with tubulin heterodimers. It is expressed throughout the nervous system after P14 and is specifically involved in filopodia and growth cone morphology in neurons. These mice exhibit abnormally small Purkinje cells in the cerebellum during the period of dendritic branching. They also display long-term depression of excitatory synaptic transmissions. This strain may be useful for studying nervous system developmental disorders.
A targeting vector was designed to replace exons 2-6 of the dihydropyrimidinase-like 5 (Dpysl4 or Crmp5) gene with an internal ribosome entry site (IRES)-β-galactosidase (lacZ) followed by a neomycin resistance cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred with C57BL/6 females for germline transmission. A chimeric male offspring was then bred to 129/SvE females. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | Dpysl5 is normally expressed throughout the nervous system after P14 and is specifically involved in filopodia and growth cone morphology in neurons. |
Allele Name | targeted mutation 1, Yoshio Goshima |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Dpysl5, dihydropyrimidinase-like 5 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | Dpysl5 is normally expressed throughout the nervous system after P14 and is specifically involved in filopodia and growth cone morphology in neurons. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 5 |
Molecular Note | Exons 2 through 6 were replaced with an IRES/lacZ/neo cassette. The absence of protein expression was confirmed by western blot analysis on brain extracts. |
Mutations Made By | Yoshio Goshima, Yokohama City University Graduate School |
When maintaining a live colony, homozygous mice may be bred together.
When using the 129;B6-Dpysl5tm1Gosh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016928 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Dpysl5<tm1Gosh> |
Frozen Mouse Embryo | 129;B6-Dpysl5<tm1Gosh>/J | $2595.00 |
Frozen Mouse Embryo | 129;B6-Dpysl5<tm1Gosh>/J | $2595.00 |
Frozen Mouse Embryo | 129;B6-Dpysl5<tm1Gosh>/J | $3373.50 |
Frozen Mouse Embryo | 129;B6-Dpysl5<tm1Gosh>/J | $3373.50 |
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