Overview

Also Known As:BTBR Overtime

The ENU induced mutation (Fbxl3^Ovtm) on the BTBR inbred background results in an extended circadian period. These mice may be useful for studying circadian rhythm modulation.

Donating Investigator

Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr

Genetic overview

Genetic Background	Generation

Itpr3^tf

Allele Type	Gene Symbol	Gene Name
Spontaneous	Itpr3	inositol 1,4,5-triphosphate receptor 3

T⁺

Allele Type	Gene Symbol	Gene Name
Not Applicable	T	brachyury, T-box transcription factor T

Allele Type	Gene Symbol	Gene Name
Chemically induced (ENU)	Fbxl3	F-box and leucine-rich repeat protein 3

Research Applications

Research Tools
Neurobiology Research
Cell Biology Research
Dermatology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Ovtm ENU-induced mutants contain an A to G transition at nucleotide in exon 5 of the F-box and leucine-rich repeat protein 3 (Fbxl3) gene that defines an amino acid change from isoleucine to threonine at residue 364. While homozygous males are viable, fertile, and normal in size, homozygous females are viable and normal in size, but are often infertile. FBXL3 is part of the SKP1-CUL1-F-box-protein (SCF) ubiquitin protein ligase complex which mediates phosphorylation-dependent ubiquitination. Homozygotes have a long circadian period of ~26 hours. These mice may be useful for studying circadian rhythm modulation.

Development

Male BTBR T⁺ Itpr3^tf/J mice (Stock No. 002282) were treated with N-ethyl-N-nitrosourea (ENU) and then bred to BTBR T⁺ Itpr3^tf/J females. The resulting male pups were then bred to BTBR T⁺ Itpr3^tf/J females to obtain second generation female mice, which were subsequently backcrossed to first generation males to obtain third generation mice. A mutagenesis screen was then performed to identify mutations that alter circadian periodicity. One of these mutants, with a 25.8 hour period length length segregates in autosomal semi-dominant-manner was termed Overtime (Ovtm). Further studies revealed the F-box and leucine-rich repeat protein 3 (Fbxl3) gene as a candidate gene for Ovtm. In Ovtm mice, Fbxl3 contains a A to G transition in exon 5 of the coding region that defines an amino acid change from isoleucine to threonine at residue 364. These mice have been backcrossed to BTBR T⁺ Itpr3^tf/J prior to arrival at The Jackson Laboratory.

Control Suggestions

Heterozygote from the colony
Wild-type from the colony

Approximate Controls

002282 BTBR T⁺ Itpr3^tf/J

Additional Information

Considerations for Choosing Controls

Selected References

Center for Functional Genomics (CFG) at Northwestern University. 2003. Heritable mouse mutants from Neurogenomics Project at Northwestern University MGI Direct Data SubmissionMGI: J:83419

View All References

Genetics

Itpr3^tf

Allele Symbol: Itpr3^tf

Allele Name	tufted
Allele Type	Spontaneous
Allele Synonym(s)	tf
Gene Symbol and Name	Itpr3, inositol 1,4,5-triphosphate receptor 3
Gene Synonym(s)
Strain of Origin	BTBR
Chromosome	17
General Note	This allele was recovered from a Harwell testing stock carrying multiple recessive markers in an undefined background. (J:273)
Molecular Note	Complementation mapping was used to demonstrate that this spontaneous mutation was an allele of Itpr3. Sequencing revealed a 12 bp deletion in Exon23 (Chr17: 27238069, Build 38.1) which codes for amino acids 983-986. This mutation arose early in the history of the BTBR strain (in or soon after 1956) and is not found in 18 other strains (129P2/OlaHsd, 129S1/SvImJ, 129S5/SvEvBrd, A/J, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6NJ, CAST/EiJ, CBA/J, DBA/2J, FVB/NJ, LP/J, NOD/ShiLtJ, NZO/HlLtJ, PWK/PhJ, SPRET/EiJ and WSB/EiJ)

T⁺

Allele Symbol: T⁺

Allele Name	wild type
Allele Type	Not Applicable
Allele Synonym(s)
Gene Symbol and Name	T, brachyury, T-box transcription factor T
Gene Synonym(s)
Strain of Origin	Not Specified
Chromosome	17

Fbxl3^Ovtm

Allele Symbol: Fbxl3^Ovtm

Allele Name	overtime
Allele Type	Chemically induced (ENU)
Allele Synonym(s)
Gene Symbol and Name	Fbxl3, F-box and leucine-rich repeat protein 3
Gene Synonym(s)
Strain of Origin	BTBR T⁺ Itpr3^tf/J
Chromosome	14
Molecular Note	This phenotypic mutant was identified in an ENU mutagenesis screen. Subsequently, a single A to G transition was identified in exon 5. This results in the conversion of amino acid residue 364 from isoleucine to threonine.
Mutations Made By	Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Mutagenesis and Transgenesis: Production of Chemical Mutations
Neurobiology Research
- Circadian Rhythms
Cell Biology Research
- Post-translational Processing

Genotype: Itpr3^tf related

Dermatology Research
- Skin and Hair Texture Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Fbxl3^Ovtm/Fbxl3^Ovtm
involves: BTBR * C57BL/6J

behavior/neurological phenotype

prolonged circadian behavior period
- period length is 26 hr

Genotype: Fbxl3^Ovtm/Fbxl3^Ovtm
involves: BTBR/J * C3H/HeJ * C57BL/6J

behavior/neurological phenotype

prolonged circadian behavior period
- circadian period is extended to 26.2 hours compared to 25.8 in wild-type mice

The following phenotype relates to a compound genotype created using this strain

Genotype: Fbxl3^Ovtm/Fbxl3⁺
BTBR T<+> Itpr3/Itpr3-Fbxl3/Nwu

behavior/neurological phenotype

prolonged circadian behavior period
- heterozygous mutant mice display a longer free running period than that of wild-type mice (23.97 hrs vs.23.27 hrs ¿ 0.13 sd)

Genotype: Fbxl3^Ovtm/Fbxl3^Ovtm
BTBR T<+> Itpr3/Itpr3-Fbxl3/Nwu

behavior/neurological phenotype

prolonged circadian behavior period
- homozygous mutant mice display a longer free running period than that of wild-type mice (25.88 hrs vs.23.27 hrs ¿ 0.13 sd)

References

Center for Functional Genomics (CFG) at Northwestern University. 2003. Heritable mouse mutants from Neurogenomics Project at Northwestern University MGI Direct Data SubmissionMGI: J:83419

Additional - Fbxl3^Ovtm related

Additional - Itpr3^tf related

Additional - T⁺ related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Fbxl3-ARMS-alternate2
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous females may be bred to homozygous males. The donating investigator reports that homozygous females are often infertile.
- Additional Breeding and Husbandry Support
Citation
When using the BTBR Overtime mouse strain in a publication, please cite the originating article(s) and include JAX stock #016926 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Fbxl3<Ovtm>, 1 pair minimum

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Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:BTBR Overtime

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Itpr3tf

Allele Symbol: Itpr3tf

T+

Allele Symbol: T+

Fbxl3Ovtm

Allele Symbol: Fbxl3Ovtm

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Itpr3tf related

Mammalian Phenotype Terms by Genotype

Genotype: Fbxl3Ovtm/Fbxl3Ovtminvolves: BTBR * C57BL/6J

behavior/neurological phenotype

Genotype: Fbxl3Ovtm/Fbxl3Ovtminvolves: BTBR/J * C3H/HeJ * C57BL/6J

behavior/neurological phenotype

Genotype: Fbxl3Ovtm/Fbxl3+BTBR T<+> Itpr3/Itpr3-Fbxl3/Nwu

behavior/neurological phenotype

Genotype: Fbxl3Ovtm/Fbxl3OvtmBTBR T<+> Itpr3/Itpr3-Fbxl3/Nwu

behavior/neurological phenotype

References

Additional - Fbxl3Ovtm related

Additional - Itpr3tf related

Additional - T+ related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Itpr3^tf

Allele Symbol: Itpr3^tf

T⁺

Allele Symbol: T⁺

Fbxl3^Ovtm

Allele Symbol: Fbxl3^Ovtm

Genotype: Itpr3^tf related

Genotype: Fbxl3^Ovtm/Fbxl3^Ovtm
involves: BTBR * C57BL/6J

Genotype: Fbxl3^Ovtm/Fbxl3^Ovtm
involves: BTBR/J * C3H/HeJ * C57BL/6J

Genotype: Fbxl3^Ovtm/Fbxl3⁺
BTBR T<+> Itpr3/Itpr3-Fbxl3/Nwu

Genotype: Fbxl3^Ovtm/Fbxl3^Ovtm
BTBR T<+> Itpr3/Itpr3-Fbxl3/Nwu

Additional - Fbxl3^Ovtm related

Additional - Itpr3^tf related

Additional - T⁺ related