The ENU induced mutation (Fbxl3Ovtm) on the BTBR inbred background results in an extended circadian period. These mice may be useful for studying circadian rhythm modulation.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical CtrRead More +
|Allele Type||Gene Symbol||Gene Name|
|Chemically induced (ENU)||Fbxl3||F-box and leucine-rich repeat protein 3|
|Allele Type||Gene Symbol||Gene Name|
|Spontaneous||Itpr3||inositol 1,4,5-triphosphate receptor 3|
|Allele Type||Gene Symbol||Gene Name|
Ovtm ENU-induced mutants contain an A to G transition at nucleotide in exon 5 of the F-box and leucine-rich repeat protein 3 (Fbxl3) gene that defines an amino acid change from isoleucine to threonine at residue 364. While homozygous males are viable, fertile, and normal in size, homozygous females are viable and normal in size, but are often infertile. FBXL3 is part of the SKP1-CUL1-F-box-protein (SCF) ubiquitin protein ligase complex which mediates phosphorylation-dependent ubiquitination. Homozygotes have a long circadian period of ~26 hours. These mice may be useful for studying circadian rhythm modulation.
Male BTBR T+ Itpr3tf/J mice (Stock No. 002282) were treated with N-ethyl-N-nitrosourea (ENU) and then bred to BTBR T+ Itpr3tf/J females. The resulting male pups were then bred to BTBR T+ Itpr3tf/J females to obtain second generation female mice, which were subsequently backcrossed to first generation males to obtain third generation mice. A mutagenesis screen was then performed to identify mutations that alter circadian periodicity. One of these mutants, with a 25.8 hour period length length segregates in autosomal semi-dominant-manner was termed Overtime (Ovtm). Further studies revealed the F-box and leucine-rich repeat protein 3 (Fbxl3) gene as a candidate gene for Ovtm. In Ovtm mice, Fbxl3 contains a A to G transition in exon 5 of the coding region that defines an amino acid change from isoleucine to threonine at residue 364. These mice have been backcrossed to BTBR T+ Itpr3tf/J prior to arrival at The Jackson Laboratory.
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||Fbxl3, F-box and leucine-rich repeat protein 3|
|Gene Synonym(s)||AU041772; AW212966; F-box and leucine-rich repeat protein 3a; FBL3; FBL3A; FBXL3A; Fbl3a; Fbxl3a; Fbxl3a; Ovtm; Ovtm; Play68; Play68; expressed sequence AU041772; expressed sequence AW212966; overtime; play 68|
|Strain of Origin||BTBR T+ Itpr3tf/J|
|Molecular Note||This phenotypic mutant was identified in an ENU mutagenesis screen. Subsequently, a single A to G transition was identified in exon 5. This results in the conversion of amino acid residue 364 from isoleucine to threonine.|
|Mutations Made By|| |
Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr
|Gene Symbol and Name||Itpr3, inositol 1,4,5-triphosphate receptor 3|
|Gene Synonym(s)||IP3R; IP3R3; IP3R3X; Ip3r3; Ip3r3; Itpr-3; Itpr-3; inositol 1,4,5-triphosphate receptor, type III; tf; tf; tufted|
|Strain of Origin||BTBR|
|General Note||This allele was recovered from a Harwell testing stock carrying multiple recessive markers in an undefined background. (J:273)|
|Molecular Note||Complementation mapping was used to demonstrate that this spontaneous mutation was an allele of Itpr3. Sequencing revealed a 12 bp deletion in Exon23 (Chr17: 27238069, Build 38.1) which codes for amino acids 983-986. This mutation arose early in the history of the BTBR strain (in or soon after 1956) and is not found in 18 other strains (129P2/OlaHsd, 129S1/SvImJ, 129S5/SvEvBrd, A/J, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6NJ, CAST/EiJ, CBA/J, DBA/2J, FVB/NJ, LP/J, NOD/ShiLtJ, NZO/HlLtJ, PWK/PhJ, SPRET/EiJ and WSB/EiJ)|
When maintaining a live colony, heterozygous females may be bred to homozygous males. The donating investigator reports that homozygous females are often infertile.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.