The ENU induced mutation (Fbxl3Ovtm) on the BTBR inbred background results in an extended circadian period. These mice may be useful for studying circadian rhythm modulation.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Itpr3 | inositol 1,4,5-triphosphate receptor 3 |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Not Applicable | T | brachyury, T-box transcription factor T |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Chemically induced (ENU) | Fbxl3 | F-box and leucine-rich repeat protein 3 |
Ovtm ENU-induced mutants contain an A to G transition at nucleotide in exon 5 of the F-box and leucine-rich repeat protein 3 (Fbxl3) gene that defines an amino acid change from isoleucine to threonine at residue 364. While homozygous males are viable, fertile, and normal in size, homozygous females are viable and normal in size, but are often infertile. FBXL3 is part of the SKP1-CUL1-F-box-protein (SCF) ubiquitin protein ligase complex which mediates phosphorylation-dependent ubiquitination. Homozygotes have a long circadian period of ~26 hours. These mice may be useful for studying circadian rhythm modulation.
Male BTBR T+ Itpr3tf/J mice (Stock No. 002282) were treated with N-ethyl-N-nitrosourea (ENU) and then bred to BTBR T+ Itpr3tf/J females. The resulting male pups were then bred to BTBR T+ Itpr3tf/J females to obtain second generation female mice, which were subsequently backcrossed to first generation males to obtain third generation mice. A mutagenesis screen was then performed to identify mutations that alter circadian periodicity. One of these mutants, with a 25.8 hour period length length segregates in autosomal semi-dominant-manner was termed Overtime (Ovtm). Further studies revealed the F-box and leucine-rich repeat protein 3 (Fbxl3) gene as a candidate gene for Ovtm. In Ovtm mice, Fbxl3 contains a A to G transition in exon 5 of the coding region that defines an amino acid change from isoleucine to threonine at residue 364. These mice have been backcrossed to BTBR T+ Itpr3tf/J prior to arrival at The Jackson Laboratory.
Allele Name | tufted |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | tf |
Gene Symbol and Name | Itpr3, inositol 1,4,5-triphosphate receptor 3 |
Gene Synonym(s) | |
Strain of Origin | BTBR |
Chromosome | 17 |
General Note | This allele was recovered from a Harwell testing stock carrying multiple recessive markers in an undefined background. (J:273) |
Molecular Note | Complementation mapping was used to demonstrate that this spontaneous mutation was an allele of Itpr3. Sequencing revealed a 12 bp deletion in Exon23 (Chr17: 27238069, Build 38.1) which codes for amino acids 983-986. This mutation arose early in the history of the BTBR strain (in or soon after 1956) and is not found in 18 other strains (129P2/OlaHsd, 129S1/SvImJ, 129S5/SvEvBrd, A/J, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6NJ, CAST/EiJ, CBA/J, DBA/2J, FVB/NJ, LP/J, NOD/ShiLtJ, NZO/HlLtJ, PWK/PhJ, SPRET/EiJ and WSB/EiJ) |
Allele Name | overtime |
---|---|
Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | |
Gene Symbol and Name | Fbxl3, F-box and leucine-rich repeat protein 3 |
Gene Synonym(s) | |
Strain of Origin | BTBR T+ Itpr3tf/J |
Chromosome | 14 |
Molecular Note | This phenotypic mutant was identified in an ENU mutagenesis screen. Subsequently, a single A to G transition was identified in exon 5. This results in the conversion of amino acid residue 364 from isoleucine to threonine. |
Mutations Made By | Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr |
When maintaining a live colony, heterozygous females may be bred to homozygous males. The donating investigator reports that homozygous females are often infertile.
When using the BTBR Overtime mouse strain in a publication, please cite the originating article(s) and include JAX stock #016926 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Fbxl3<Ovtm>, 1 pair minimum |
Frozen Mouse Embryo | BXD42/TyJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | BXD42/TyJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | BXD42/TyJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | BXD42/TyJ Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.