These transgenic mice express human complement receptor 1, CR1, on erythrocytes. Erythrocytes from transgenic mice will adhere to beads coated with human or mouse serum complement, an attribute that may prove useful in studies of immune adherence and pathogen clearance.
Robert W. Finberg, University of Massachusetts
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These transgenic mice express the human complement component (3b/4b) receptor 1 (Knops blood group), CR1, under the control of the mouse Gata1, GATA binding protein 1, promoter. Transgene expression is detected on erythrocytes by flow cytometry. Unlike erythrocytes from wildtype controls, erythrocytes from transgenic mice will adhere beads coated with human or mouse serum complement. Transgenic erythrocytes exhibit increased adherence of pneumococci and type 5 adenovirus as well as increased Plasmodium falciparum merozoite invasion when compared to wildtype controls. The donating investigator reports that this transgene is locatd on the X chromosomse. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
A transgenic construct containing a human CR1 gene, under the control of the mouse Gata1, GATA binding protein 1, promoter was injected into fertilized SJL X C57BL/6 mouse eggs. The resulting transgenic mice were crossed to C57BL/6J mice for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | CR1, complement C3b/C4b receptor 1 (Knops blood group), human |
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Site of Expression |
Allele Name | transgene insertion 1, Robert W Finberg |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Gata1-CR1)1Rwf, transgene insertion 1, Robert W Finberg |
Gene Synonym(s) | |
Promoter | Gata1, GATA binding protein 1, mouse, laboratory |
Expressed Gene | CR1, complement C3b/C4b receptor 1 (Knops blood group), human |
Strain of Origin | (SJL x C57BL/6)F1 |
Chromosome | UN |
Molecular Note | The transgene contains a human CR1 gene, under the control of the mouse Gata1, GATA binding protein 1, promoter. Line 1 was generated. |
Mutations Made By | Robert Finberg, University of Massachusetts |
When maintaining a live colony, these mice can be bred as hemizygotes. This transgene is X-linked.
When using the B6.Cg-Tg(Gata1-CR1)1Rwf/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016911 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Gata1-CR1)1Rwf |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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