Mice homozygous for the spontaneous recessive small roller mutation can usually be identified by 2 weeks of age by their difficulty in walking, which persists into adulthood. The hind legs splay, the gait has a severe side-to-side sway with the body held lower than normal, and homozygotes often tumble over. They have difficulty righting. Homozygotes are also smaller than normal, half normal size at wean age and growing to three quarters normal size as adults. Homozygous females have smaller than normal litter sizes, often produce fewer than expected numbers of homozygotes, and have a high number of stillborn and lost newborns. Heterozygous intercrosses also produce a high number of stillborn and lost newborns. Male homozygotes might not be fertile.
The small roller mutation arose spontaneously in the CXB10/HiAJ recombinant inbred strain at The Jackson Laboratory when that strain was at generation F59. This mutant subline has been maintained by sibling inbreeding on this background ever since.
|Allele Name||small roller|
|Gene Symbol and Name||Cacna1a, calcium channel, voltage-dependent, P/Q type, alpha 1A subunit|
|Strain of Origin||CXB10/HiAJ|
|Molecular Note||This spontaneous mutation has a an extra G inserted in the coding region of Cacna1a in the poly(G) stretch at coding nucleotides 106-110 of the cDNA (NM_001252060).|
Due to the fragility and diminutive size of homozygous pups meal should be placed within easy reach and weaning should be delayed beyond the usual three week wean age. Homozygous females will breed, but have reduced litter size and increased stillborn and pre-wean death of pups. Homozygous males have not been found to breed. This strain can be maintained through progeny tested heterozygotes.
When using the CXB10/HiAJ-Cacna1asmrl/GrsrJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #016887 in your Materials and Methods section.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.