Mice homozygous for the spontaneous recessive small roller mutation can usually be identified by 2 weeks of age by their difficulty in walking, which persists into adulthood. The hind legs splay, the gait has a severe side-to-side sway with the body held lower than normal, and homozygotes often tumble over. They have difficulty righting. Homozygotes are also smaller than normal, half normal size at wean age and growing to three quarters normal size as adults. Homozygous females have smaller than normal litter sizes, often produce fewer than expected numbers of homozygotes, and have a high number of stillborn and lost newborns. Heterozygous intercrosses also produce a high number of stillborn and lost newborns. Male homozygotes might not be fertile.
The small roller mutation arose spontaneously in the CXB10/HiAJ recombinant inbred strain at The Jackson Laboratory when that strain was at generation F59. This mutant subline has been maintained by sibling inbreeding on this background ever since.
|Allele Name||small roller|
|Allele Synonym(s)||small roller; Cacna1asmrl|
|Gene Symbol and Name||Cacna1a, calcium channel, voltage-dependent, P/Q type, alpha 1A subunit|
|Gene Synonym(s)||alpha1A; BccA1; calcium channel alpha 1a; nmf352; CACNL1A4; nmf352; EA2; CAV2.1; APCA; rbA-1; BI; MHP; MHP1; rkr; la; HPCA; neuroscience mutagenesis facility, 352; SCA6; FHM; tg; Ccha1a; tottering; leaner; Ccha1a; rocker; small roller; smrl; smrl; Cacnl1a4; Cav2.1; EIEE42|
|Strain of Origin||CXB10/HiAJ|
|Molecular Note||This spontaneous mutation has a CG dinuceotide insertion in the coding region of Cacna1a at position 105 of the cDNA (NM_001252060).|
Due to the fragility and diminutive size of homozygous pups meal should be placed within easy reach and weaning should be delayed beyond the usual three week wean age. Homozygous females will breed, but have reduced litter size and increased stillborn and pre-wean death of pups. Homozygous males have not been found to breed. This strain can be maintained through progeny tested heterozygotes.
When using the CXB10/HiAJ-Cacna1asmrl/GtsrJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #016887 in your Materials and Methods section.
|Heterozygous or wildtype for Cacna 1a<smrl>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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