Overview

Also Known As:SST-tTA2-D

SST-tTA2-D knock-in/knock-out mice have synthetic modified tetracycline-regulated transactivator (tTA2^S) gene expression directed to Sst-expressing cells by the endogenous promoter/enhancer elements of the somatostatin locus. These SST-tTA2-D mice are a Tet-Off tool that allows dox-conditional expression of genes in Sst-expressing cells/tissues.

Donating Investigator

Hongkui Zeng, Allen Institute for Brain Science

Genetic overview

Genetic Background	Generation

Sst^{tm1.2(tTA2)Hze}

Allele Type	Gene Symbol	Gene Name
Targeted (Transactivator)	Sst	somatostatin

View Genetics

Research Applications

Research Tools
Neurobiology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

The SST-tTA2-D (SST-tTA2-Δ) knock-in/knock-out allele both abolishes Sst gene function and expresses a synthetic modified tetracycline-regulated transactivator (tTA2^S) from the somatostatin promoter/enhancer elements.

Heterozygous mice are viable and fertile. Although the donating investigator did not evaluate the phenotype of homozygous animals to date (October 2011), homozygous mice may be expected to have a knockout phenotype similar to other null mutations of this gene and may exhibit nervous system and metabolic abnormalities.

When SST-tTA2-D mice are bred with mice carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE, TetRE or tetO), expression of the target gene in the somatostatin-expressing cells may be conditionally abolished with administration of the tetracycline analog doxycycline (dox) in the double mutant offspring. Specifically, the donating investigator reports that SST-tTA2-D activates tetO-regulated gene expression in scattered neural populations, in a pattern that resembles Sst expression. The donating investigator did not examine tTA2^S activity in tissues other than brain.

Development

A targeting vector was used to insert a synthetic modified tetracycline-regulated transactivator (tTA2^S) gene and 3' UTR region of the somatostatin exon 2 into the ATG transcriptional start site of the somatostatin gene (Sst). Specifically, the targeting vector was designed with (from 5' to 3') an frt3 site, a Cre recombinase gene, a bovine growth hormone polyA sequence, a second frt3 site, a tTA2^S gene, the 3' UTR region from somatostatin exon 2, an AttB site, PGK-Neo-polyA cassette, an frt5 site, an RNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. This construct was electroporated into (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric animals were bred to C57BL/6J mice to generate the SST-Cre-pA-tTA2 mutant colony. To remove the frt-flanked Cre recombinase and polyA sequence, the mice were bred to ROSA26::FLPe deleter mice (129S4 genetic background; Stock No. 003946). The SST-tTA2 offspring were then bred to PhiC31-expressing mice (C57BL/6J congenic background; see Stock No. 007743) to replace the PGK-Neo and hygromycin-polyA cassettes with the recombined AttB/AttP site (AttL). The resulting SST-tTA2-D (or SST-tTA2-Δ) mice have a single frt3 site, tTA2^S, the 3' UTR from somatostatin exon 2, and AttL site targeted into the somatostatin ATG site. The SST-tTA2-Δ mice were subsequently backcrossed to C57BL/6J wildtype mice for at least five additional generations (and the FLP-expressing mutation and PhiC31 transgene was removed) prior to sending to The Jackson Laboratory Repository. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the living colony.

Expression Data

Expressed Gene	tTA, tetracycline-controlled transactivator, E. coli
Site of Expression	Sst^{tm1.2(tTA2)Hze} activates tetO-regulated gene expression in scattered neural populations, in a pattern that resembles Sst expression.

Control Suggestions

Wild-type from the colony
000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Zeng H. 2011. Direct Data Submission 2011/10/17 MGI Direct Data SubmissionMGI: J:176570

View All References

Genetics

Sst^{tm1.2(tTA2)Hze}

Allele Symbol: Sst^{tm1.2(tTA2)Hze}

Allele Name	targeted mutation 1.2, Hongkui Zeng
Allele Type	Targeted (Transactivator)
Allele Synonym(s)	SST-tTA2-D; SST-tTA2-DELTA; SST-tTA2-deltaNEO
Gene Symbol and Name	Sst, somatostatin
Gene Synonym(s)
Expressed Gene	tTA, tetracycline-controlled transactivator, E. coli
Site of Expression	Sst^{tm1.2(tTA2)Hze} activates tetO-regulated gene expression in scattered neural populations, in a pattern that resembles Sst expression.
Strain of Origin	(129S6/SvEvTac x C57BL/6NCrl)F1
Chromosome	16
Molecular Note	A targeting vector used to insert a synthetic modified tetracycline-regulated transactivator (tTA2S) gene and 3' UTR region of the somatostatin exon 2 into the ATG transcriptional start site of the somatostatin gene (Sst). Specifically, the targeting vector was designed with (from 5' to 3') an frt3 site, a Cre recombinase gene, a bovine growth hormone polyA sequence, a second frt3 site, a tTA2S gene, the 3' UTR region from somatostatin exon 2, an AttB site, PGK-Neo-polyA cassette, an frt5 site, an RNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site.
Mutations Made By	Hongkui Zeng, Allen Institute for Brain Science

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Tissue/Cell Markers
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers: neurons
  - Mutagenesis and Transgenesis: Tetop Tet System
- Neurobiology Research
  - cell marker
  - Tetop Tet System
- Cancer Research
  - Tetop Tet System
- Tet Expression Systems
  - tTA/rtTA Expressing Strains
Neurobiology Research
- Tet Expression System
  - tTA/rtTA Expressing Strains

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Sst^{tm1.2(tTA2)Hze}/Sst^{tm1.2(tTA2)Hze}
B6.Cg-Sst

mammalian phenotype

no phenotypic analysis

References

Zeng H. 2011. Direct Data Submission 2011/10/17 MGI Direct Data SubmissionMGI: J:176570

Additional - Sst^{tm1.2(tTA2)Hze} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Sst
- Separated MCA:SstMCA
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred to wildtype siblings from the colony or to C57BL/6J inbred mice (Stock No. 000664). The homozygous phenotype is unknown (September 2011).
- Additional Breeding and Husbandry Support
Citation
When using the SST-tTA2-D mouse strain in a publication, please cite the originating article(s) and include JAX stock #016868 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Not-For-Profit & Academic Pricing

Service/Product	Description	Price
	Heterozygous or wildtype for Sst<tm1.2(tTA2)Hze>

Related Products and Services

Frozen Mouse Embryo	B6.Cg-Sst<tm1.2(tTA2)Hze>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.Cg-Sst<tm1.2(tTA2)Hze>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.Cg-Sst<tm1.2(tTA2)Hze>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6.Cg-Sst<tm1.2(tTA2)Hze>/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Use of MICE only available to non-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Also Known As:SST-tTA2-D

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Ssttm1.2(tTA2)Hze

Allele Symbol: Ssttm1.2(tTA2)Hze

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Ssttm1.2(tTA2)Hze/Ssttm1.2(tTA2)HzeB6.Cg-Sst

mammalian phenotype

References

Additional - Ssttm1.2(tTA2)Hze related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Not-For-Profit & Academic Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Sst^{tm1.2(tTA2)Hze}

Allele Symbol: Sst^{tm1.2(tTA2)Hze}

Genotype: Sst^{tm1.2(tTA2)Hze}/Sst^{tm1.2(tTA2)Hze}
B6.Cg-Sst

Additional - Sst^{tm1.2(tTA2)Hze} related