These floxed mutant mice contain loxP sites flanking exons 2-3 of the Ccl2 gene which has been modified with a red fluorescent protein gene (mcherry). Fluorescence is seen in all Ccl2-expressing cells. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-3, as well as RFP, deleted in the cre-expressing tissue.
Eric G Pamer, Memorial Sloan-Kettering Cancer Center
Genetic Background | Generation |
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N10pN1+N1F6
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Ccl2 | chemokine (C-C motif) ligand 2 |
These Ccl2-RFPlox/lox mice contain loxP sites flanking exons 2-3 of the chemokine (C-C motif) ligand 2 (Ccl2 or Mcp1) gene, as well as an HA peptide followed by an aphthovirus 2A cleavage site and a cleavable red fluorescent protein (mcherry) at the 3' end of exon 3. Homozygous mice are viable and fertile. MCP1 is a chemokine required for the movement of monocytes from bone marrow into the blood stream during their recruitment to sites of injury and infection. Fluorescence is seen in all MCP1 expressing cells. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-3, as well as RFP expression, deleted in the cre-expressing tissue. Deletion of MCP1 results in decreased monocyte emigration from the bone marrow upon LPS stimulation and
increased susceptibility to infection. These mice may be useful for studying emigration of inflammatory monocytes from the bone marrow after infection.
When bred to mice carrying Tg(Tek-cre)12Flv (Stock No. 004128) and induced by LPS or bacterial infection, Cre recombinase expression in endothelial cells results in abnormal monocyte migration.
When bred to mice carrying Tg(Nes-cre)1Kln (Stock No. 003771) and induced by LPS or bacterial infection, Cre recombinase expression in the nervous system results in abnormal monocyte migration.
A targeting vector was designed to insert a loxP site upstream of exon 2 and a second loxP site downstream of exon 3 of the chemokine (C-C motif) ligand 2 (Ccl2 or Mcp1) gene. The targeting vector also inserted an HA peptide followed by an aphthovirus 2A cleavage site, a cleavable red fluorescent protein (mcherry), and a frt-flanked neomycin (neo) resistance cassette at the 3' end of exon 3. The construct was electroporated into B6(Cg)-Tyrc-2J/J derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6J mice. Offspring, heterozygous for this Ccl2 allele, were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 005703) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene, resulting in a colony homozygous for the Ccl2-RFPlox/lox allele. These mice were backcrossed to C57BL/6J mice for at least 10 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Allele Name | targeted mutation 1.1 Eric G Pamer |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Ccl2-RFPflox; Mcp1f |
Gene Symbol and Name | Ccl2, chemokine (C-C motif) ligand 2 |
Gene Synonym(s) | |
Strain of Origin | B6(Cg)-Tyrc-2J |
Chromosome | 11 |
Molecular Note | A loxP site was inserted upstream of exon 2. Exon 3 was replaced with a modified exon 3 with an HA-2A cleavable mCherry and 3' loxP site. A floxed neo cassette used for selection purposes was removed by cre-mediated recombination. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Ccl2-RFPflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #016849 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ccl2<tm1.1Pame> |
Frozen Mouse Embryo | B6.Cg-Ccl2<tm1.1Pame>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Ccl2<tm1.1Pame>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Ccl2<tm1.1Pame>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Ccl2<tm1.1Pame>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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