These floxed mutant mice possess loxP sites flanking exon 4 of the Igf1 gene and a neomycin resistance (neo) cassette. This strain may be useful for studying postnatal growth and development.
Dr. Derek LeRoith, Mount Sinai School of Medicine
Genetic Background | Generation |
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N11F2
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Igf1 | insulin-like growth factor 1 |
These mutant mice feature loxP sites flanking exon 4 of the insulin-like growth factor 1 (Igf1) gene and a neomycin resistance cassette. Homozygotes for this allele are viable, fertile, and normal in size. IGF1 promotes cell survival and cell proliferation by activating the tyrosine kinase transmembrane receptor, IGF-1R. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in cre-expressing tissues. For example, when these mice are bred to Tg(Alb1-cre)1Dlr/J mice (Stock No. 016832 or Stock No. 016833), IGF1 expression is abolished in liver. This strain may be useful for studying postnatal growth and development.
When bred to a strain expressing Cre recombinase in the early mouse embryo (see Stock No. 003724 for example), this mutant mouse strain may be useful in studies of early development.
A targeting vector was designed to insert a loxP site upstream of exon 4 and a neomycin resistance (neo) cassette and second loxP site downstream of exon 4 of the insulin-like growth factor 1 (Igf1) gene. The construct was electroporated into 129-derived embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred with C57BL/6J females, and their offspring were subsequently crossed to FVB-Tg(Alb1-cre)1Dlr mice. The donating investigator reported that these mice were backcrossed to C57BL/6J inbred mice for at least 9 generations (see SNP note below). Upon arrival, the mice were bred to C57BL/6J inbred mice (Stock No. 000664) for one generation and transgene was selectively bred out of the line.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Allele Name | targeted mutation 1, Derek LeRoith |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Igf1floxed; IGF-I loxP; IGF-Iflox; LID; LI-IGF-I- |
Gene Symbol and Name | Igf1, insulin-like growth factor 1 |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 10 |
General Note | ES cells = 129/Sv |
Molecular Note | A neomycin cassette and loxP sites were inserted flanking exon 4. The introduction of these mutations had no effect on the normal function of the gene. |
Mutations Made By | Dr. Derek LeRoith, Mount Sinai School of Medicine |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Igf1flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #016831 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Igf1<tm1Dlr> |
Frozen Mouse Embryo | B6.129(FVB)-Igf1<tm1Dlr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(FVB)-Igf1<tm1Dlr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129(FVB)-Igf1<tm1Dlr>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129(FVB)-Igf1<tm1Dlr>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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