KitW-v homozygotes resemble KitW homozygotes in color, anemia, and germ cells, but many of them survive to maturity. Mice on this background exhibit poor viability, mast cell defects, decreased airway hyperresponsiveness and sterility.
David Beier, Harvard
Homozygote: Kit mice possess pleiotropic defects in pigment-forming cells, germ cells, RBC's and mast cells. In addition, they exhibit impaired resistance to parasitic infection and an intrinsic progenitor cell defect. KitW-v homozygotes resemble KitW homozygotes in color, anemia, and germ cells, but many of them survive to maturity. On the A/J background, the classic black-eyed albino phenotype cannot be used to assess genotype. The donating investigator reports that mice on this background exhibit poor viability, mast cell defects, decreased airway hyperresponsiveness and sterility. On the hybrid (C57BL/6 x A/J)F1 background, KitW homozygotes are viable and exhibit decreased airway hyperresponsiveness.
Heterozygote: Not evaluated. On the albino A/J background, coat color markings are not visible.
The viable dominant spotting (W-v) mutation arose spontaneously in a strain of mice described as silvered black self (wwBBslslSS) at The Jackson Laboratory in 1937. The mutation was maintained as a cross to C57BL/6J for at least 98 generations. It was introgressed into A/J for 10 generations in the laboratory of Dr. David Beier at Brigham and Women's Hospital. All tested SNP markers outside of the congenic region were A/J in origin. The strain was donated to the MMRRC in 2011.
|Allele Name||viable dominant spotting|
|Allele Synonym(s)||KitWv; W'; W2; Wv; Wv|
|Gene Symbol and Name||Kit, KIT proto-oncogene receptor tyrosine kinase|
|Strain of Origin||silvered black strain|
|Molecular Note||A C-to-T point mutation at coding nucleotide 1979 (transcript NM_021099) results in a threonine to methionine substitution at amino acid 660 (p.T660M).|
While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation are sterile.
When using the viable dominant spotting mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #34764 in your Materials and Methods section.